6976-37-0
6976-37-0
Chemical Structure
Bis-Tris
- CAS No.: 6976-37-0
- Formula:C8H19NO5
- Molecular Weight:209.24
IUPAC Name: 2-(bis(2-hydroxyethyl)amino)-2-(hydroxymethyl)propane-1,3-diol
InChIKey: OWMVSZAMULFTJU-UHFFFAOYSA-N
SMILES: OCC(CO)(N(CCO)CCO)CO
Biological Activity: Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5)[1][2][3].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Bis-Tris | 98.0% | Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5). | ||||||||||||||||||||
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Bis-Tris (Standard) | ≥98% | Bis-Tris (Standard) is the analytical standard of Bis-Tris. This product is intended for research and analytical applications. Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5). | ||||||||||||||||||||
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Bis-Tris-d14 | Bis-Tris-d14 is the deuterium labeled Bis-Tris (HY-D0856). Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5). | |||||||||||||||||||||
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Bis-Tris-d19 | 99% | Bis-Tris-d19 is the deuterium labeled Bis-Tris. Bis-Tris is a commonly used buffer. | ||||||||||||||||||||
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References
- [1]. Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes.
- [2]. Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [Content Brief]
- [3]. Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. [Content Brief]
