Bis-Tris hydrochloride

Name: Bis-Tris hydrochloride
Brand: MedChemExpress
SKU: HY-W105681
Rating: 4.5 (1 reviews)

Cat. No.: HY-W105681 Purity: 99.60%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Bis-Tris hydrochloride is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris hydrochloride can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5).

For research use only. We do not sell to patients.

Bis-Tris hydrochloride Chemical Structure

CAS No. : 124763-51-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Bis-Tris hydrochloride:

Bis-Tris In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Bis-Tris (10 mM or 4-12% Bis-Tris gel) hydrochloride has excellent buffering capacity and, in synergy with HEPES and cacodylic acid, covers a wide pH range. It can be used for fluorescence spectroscopy analysis, circular dichroism (CD) analysis, GST pull-down binding assay, gel filtration analysis, nuclear magnetic resonance (NMR) analysis, and protein electrophoresis. Abnormal pH fluctuations can affect experimental results^[2]^[3].
Buffers containing Bis-Tris can be used to preserve purified proteins to prevent degradation (4°C)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

245.70

Formula

C₈H₂₀ClNO₅

CAS No.

124763-51-5

Appearance

Solid

Color

White to off-white

SMILES

OCCN(C(CO)(CO)CO)CCO.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (814.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0700 mL	20.3500 mL	40.7000 mL
5 mM	0.8140 mL	4.0700 mL	8.1400 mL
10 mM	0.4070 mL	2.0350 mL	4.0700 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (248 KB) HNMR (248 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes.

[2]. Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [Content Brief]

[3]. Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0700 mL	20.3500 mL	40.7000 mL	101.7501 mL
	5 mM	0.8140 mL	4.0700 mL	8.1400 mL	20.3500 mL
	10 mM	0.4070 mL	2.0350 mL	4.0700 mL	10.1750 mL
	15 mM	0.2713 mL	1.3567 mL	2.7133 mL	6.7833 mL
	20 mM	0.2035 mL	1.0175 mL	2.0350 mL	5.0875 mL
	25 mM	0.1628 mL	0.8140 mL	1.6280 mL	4.0700 mL
	30 mM	0.1357 mL	0.6783 mL	1.3567 mL	3.3917 mL
	40 mM	0.1018 mL	0.5088 mL	1.0175 mL	2.5438 mL
	50 mM	0.0814 mL	0.4070 mL	0.8140 mL	2.0350 mL
	60 mM	0.0678 mL	0.3392 mL	0.6783 mL	1.6958 mL
	80 mM	0.0509 mL	0.2544 mL	0.5088 mL	1.2719 mL
	100 mM	0.0407 mL	0.2035 mL	0.4070 mL	1.0175 mL