Bis-Tris hydrochloride
Based on 1 Customer Validation
Bis-Tris hydrochloride is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris hydrochloride can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5).
For research use only. We do not sell to patients.
- Purity: 99.60%
- CAS No.: 124763-51-5
- Formula: C8H20ClNO5
- Molecular Weight:245.70
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Bis-Tris (10 mM or 4-12% Bis-Tris gel) hydrochloride has excellent buffering capacity and, in synergy with HEPES and cacodylic acid, covers a wide pH range. It can be used for fluorescence spectroscopy analysis, circular dichroism (CD) analysis, GST pull-down binding assay, gel filtration analysis, nuclear magnetic resonance (NMR) analysis, and protein electrophoresis. Abnormal pH fluctuations can affect experimental results[2][3].
Buffers containing Bis-Tris can be used to preserve purified proteins to prevent degradation (4°C)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 124763-51-5
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Appearance Solid
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Molecular Weight 245.70
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Formula C8H20ClNO5
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Color White to off-white
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SMILES
OCCN(C(CO)(CO)CO)CCO.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 200 mg/mL (814.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [Content Brief]
[3]. Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0700 mL | 20.3500 mL | 40.7000 mL | 101.7501 mL |
| 5 mM | 0.8140 mL | 4.0700 mL | 8.1400 mL | 20.3500 mL | |
| 10 mM | 0.4070 mL | 2.0350 mL | 4.0700 mL | 10.1750 mL | |
| 15 mM | 0.2713 mL | 1.3567 mL | 2.7133 mL | 6.7833 mL | |
| 20 mM | 0.2035 mL | 1.0175 mL | 2.0350 mL | 5.0875 mL | |
| 25 mM | 0.1628 mL | 0.8140 mL | 1.6280 mL | 4.0700 mL | |
| 30 mM | 0.1357 mL | 0.6783 mL | 1.3567 mL | 3.3917 mL | |
| 40 mM | 0.1018 mL | 0.5088 mL | 1.0175 mL | 2.5438 mL | |
| 50 mM | 0.0814 mL | 0.4070 mL | 0.8140 mL | 2.0350 mL | |
| 60 mM | 0.0678 mL | 0.3392 mL | 0.6783 mL | 1.6958 mL | |
| 80 mM | 0.0509 mL | 0.2544 mL | 0.5088 mL | 1.2719 mL | |
| 100 mM | 0.0407 mL | 0.2035 mL | 0.4070 mL | 1.0175 mL |
Bis-Tris hydrochloride Related Classifications
HY-W105681 Related Classifications
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