Bis-Tris
Based on 1 Customer Validation
Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5).
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 6976-37-0
- Formula: C8H19NO5
- Molecular Weight:209.24
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Bis-Tris (10 mM or 4-12% Bis-Tris gel) has excellent buffering capacity and, in synergy with HEPES and cacodylic acid, covers a wide pH range. It can be used for fluorescence spectroscopy analysis, circular dichroism (CD) analysis, GST pull-down binding assay, gel filtration analysis, nuclear magnetic resonance (NMR) analysis, and protein electrophoresis. Abnormal pH fluctuations can affect experimental results[2][3].
Buffers containing Bis-Tris can be used to preserve purified proteins to prevent degradation (4°C)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6976-37-0
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Appearance Solid
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Molecular Weight 209.24
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Formula C8H19NO5
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Color White to off-white
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SMILES
OCC(CO)(N(CCO)CCO)CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
H2O : 200 mg/mL (955.84 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (238.96 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (272 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[2]. Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [Content Brief]
[3]. Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.7792 mL | 23.8960 mL | 47.7920 mL | 119.4800 mL |
| 5 mM | 0.9558 mL | 4.7792 mL | 9.5584 mL | 23.8960 mL | |
| 10 mM | 0.4779 mL | 2.3896 mL | 4.7792 mL | 11.9480 mL | |
| 15 mM | 0.3186 mL | 1.5931 mL | 3.1861 mL | 7.9653 mL | |
| 20 mM | 0.2390 mL | 1.1948 mL | 2.3896 mL | 5.9740 mL | |
| 25 mM | 0.1912 mL | 0.9558 mL | 1.9117 mL | 4.7792 mL | |
| 30 mM | 0.1593 mL | 0.7965 mL | 1.5931 mL | 3.9827 mL | |
| 40 mM | 0.1195 mL | 0.5974 mL | 1.1948 mL | 2.9870 mL | |
| 50 mM | 0.0956 mL | 0.4779 mL | 0.9558 mL | 2.3896 mL | |
| 60 mM | 0.0797 mL | 0.3983 mL | 0.7965 mL | 1.9913 mL | |
| 80 mM | 0.0597 mL | 0.2987 mL | 0.5974 mL | 1.4935 mL | |
| 100 mM | 0.0478 mL | 0.2390 mL | 0.4779 mL | 1.1948 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Bis-Tris Related Classifications
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