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  4. THAM acetate

THAM acetate  (Synonyms: Tris acetate; Tris(hydroxymethyl)aminomethane acetate)

Cat. No.: HY-D0227B Purity: 97.0%
COA
SDS
Handling Instructions Technical Support

THAM acetate is a low-toxicity amino alcohol buffer, a CO2-consuming proton acceptor that buffers carbon dioxide and acid both in vitro and in vivo. THAM acetate binds protons to form bicarbonate, reduces PaCO2, and induces intracellular alkalization, thereby ameliorating hypercapnia-induced elevation of pulmonary blood vessels and pulmonary arterial pressure. THAM acetate may cause PaCO2 rebound, hypoglycemia, and respiratory depression. THAM acetate removes amniotic epithelium and preserves the basement membrane, but depletes extracellular matrix and reduces the adhesion rate of limbal epithelial cells. THAM acetate can act as a CO2 carrier to enhance the productivity and carbon utilization rate of Scenedesmus obliquus. THAM acetate is a key component of buffer solutions used in various biological, cell culture, biochemical, and molecular biology applications.

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THAM acetate

THAM acetate Chemical Structure

CAS No. : 6850-28-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of THAM acetate:

THAM acetate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE THAM acetate

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  • Purity & Documentation

  • References

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Description

THAM acetate is a low-toxicity amino alcohol buffer, a CO2-consuming proton acceptor that buffers carbon dioxide and acid both in vitro and in vivo. THAM acetate binds protons to form bicarbonate, reduces PaCO2, and induces intracellular alkalization, thereby ameliorating hypercapnia-induced elevation of pulmonary blood vessels and pulmonary arterial pressure. THAM acetate may cause PaCO2 rebound, hypoglycemia, and respiratory depression. THAM acetate removes amniotic epithelium and preserves the basement membrane, but depletes extracellular matrix and reduces the adhesion rate of limbal epithelial cells. THAM acetate can act as a CO2 carrier to enhance the productivity and carbon utilization rate of Scenedesmus obliquus. THAM acetate is a key component of buffer solutions used in various biological, cell culture, biochemical, and molecular biology applications[1][2][3][4][5].

In Vitro

THAM (5 min) induces intracellular alkalization in human HepG2 hepatocytes perfused with acidotic artificial media, with a larger effect in the presence of low extracellular non-bicarbonate buffer compared to high extracellular non-bicarbonate buffer[3].
THAM (110 μL) acetate induces intracellular alkalization in human HepG2 hepatocytes bathed in acidotic human blood[3].
THAM (2-8 mmol/L; 40 min) acetate enhances CO2 gas absorptivity and total inorganic carbon storage capacity of modified BG-11 medium, with the highest TIC absorption observed at 8 mmol·L-1[5].
THAM (2-8 mmol/L; 8 days) acetate improves biomass productivity of Scenedesmus dimorphus in indoor shake flask culture, while also increasing polyunsaturated fatty acid content and showing no biodegradation over 8 days[5].
THAM (6 mmol/L; 8 days) acetate increases biomass areal productivity of Scenedesmus dimorphus by 32-33% and CO2 utilisation efficiency by 6-12% in outdoor 2 m2 raceway pond culture, while also increasing polyunsaturated fatty acid content[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

THAM acetate Related Antibodies
In Vivo

THAM (weight in kg × 9.2 mEq; i.v.; 1 hour or 3 hours) acetate reduces pulmonary vascular resistance and maintains lower pulmonary arterial pressure in lung-injured piglets with hypercapnia, even after pH rebounds to control levels, while increasing base excess and inducing a post-infusion rebound in PaCO2[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Piglets (23.0-30.5 kg body weight; acute respiratory distress syndrome induced by repeated lung lavages with 30 mL/kg 37-39 °C normal saline, eight total)[2]
Dosage: Calculated to normalize pH and provide lasting effect
Administration: i.v.; continuous infusion; 1 hour (6 animals); 3 hours (6 animals)
Result: Increased arterial pH above 7.4, decreased PaCO2, and increased base excess to 13.8 mEq/L (1-hour group) and 16.0 mEq/L (3-hour group) at 60 minutes.
Reduced pulmonary vascular resistance to 423 dyn·s/m5 (1-hour group) and 373 dyn·s/m5 (3-hour group) at 60 minutes, compared to 572 dyn·s/m5 in controls.
Rebounded PaCO2 to 15.2 kPa (1-hour group) and 22.6 kPa (3-hour group) at 6 hours post-infusion cessation.
Maintained elevated base excess at 10.2 mEq/L (1-hour group) and 27.8 mEq/L (3-hour group) at 6 hours post-infusion cessation.
Decreased pH to levels similar to controls by 6 hours post-infusion cessation.
Lowered pulmonary vascular resistance to 329 dyn·s/m5 (1-hour group) and 255 dyn·s/m5 (3-hour group) at 6 hours, compared to 450 dyn·s/m5 in controls.
Increased cardiac index to 5.0 L/min/m2 in the 3-hour group at 6 hours, compared to 3.5 L/min/m2 in controls.
Decreased arterial sodium concentration during infusion and increased arterial potassium concentration during infusion.
Showed no significant differences in lung mechanics (functional residual capacity, respiratory system compliance) between groups.
Increased lung tissue IL-6 concentration to 11.8 pg/mg protein in the 3-hour group, with no significant differences vs. controls for TNF-α or IL-1β.
Molecular Weight

181.19

Formula

C6H15NO5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCC(CO)(N)CO.OC(C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (551.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.5191 mL 27.5953 mL 55.1907 mL
5 mM 1.1038 mL 5.5191 mL 11.0381 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 97.0%

SDS (396 KB)

COA (251 KB) HNMR (121 KB) MS (120 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.5191 mL 27.5953 mL 55.1907 mL 137.9767 mL
5 mM 1.1038 mL 5.5191 mL 11.0381 mL 27.5953 mL
10 mM 0.5519 mL 2.7595 mL 5.5191 mL 13.7977 mL
15 mM 0.3679 mL 1.8397 mL 3.6794 mL 9.1984 mL
20 mM 0.2760 mL 1.3798 mL 2.7595 mL 6.8988 mL
25 mM 0.2208 mL 1.1038 mL 2.2076 mL 5.5191 mL
30 mM 0.1840 mL 0.9198 mL 1.8397 mL 4.5992 mL
40 mM 0.1380 mL 0.6899 mL 1.3798 mL 3.4494 mL
50 mM 0.1104 mL 0.5519 mL 1.1038 mL 2.7595 mL
60 mM 0.0920 mL 0.4599 mL 0.9198 mL 2.2996 mL
80 mM 0.0690 mL 0.3449 mL 0.6899 mL 1.7247 mL
100 mM 0.0552 mL 0.2760 mL 0.5519 mL 1.3798 mL
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THAM acetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

THAM6850-28-8TrisTris(hydroxymethyl)aminomethaneBiochemical Assay Reagentsdiabetic ketoacidosisacidaemiabrain injuryacute respiratory distress syndromerespiratory failuremalignant hyperthermiasevere burnsgastroenteritisrenal acidosiscardiac failureInhibitorinhibitorinhibit

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