THAM hydrochloride (≥99%, reagent grade)
Based on 2 publication(s) in Google Scholar
THAM hydrochloride (≥99%, reagent grade), also known as Tris-HCl, is a buffer commonly used in various biochemical and molecular biology applications to maintain a stable pH environment. Tris-HCl has unique chemical properties that allow it to resist changes in pH when acidic or basic substances are added, which makes it useful for stabilizing biological samples or reagents. It is commonly used in electrophoresis and protein purification procedures.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 1185-53-1
- Formula: C4H12ClNO3
- Molecular Weight:157.60
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) THAM hydrochloride (≥99%, reagent grade)
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Biological Activity
THAM hydrochloride (≥99%, reagent grade) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1185-53-1
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Appearance Solid
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Molecular Weight 157.60
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Formula C4H12ClNO3
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Color White to off-white
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SMILES
OCC(CO)(N)CO.[H]Cl
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Synonyms
Tris HCl (≥99%, reagent grade); Tris hydrochloride (≥99%, reagent grade)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Adv Mater
A Single-Cell RNA Sequencing Guided Multienzymatic Hydrogel Design for Self-Regenerative Repair in Diabetic Mandibular Defects. [Abstract]2024 Dec;36(50):e2410962. PMID: 39436107 -
iScience
Divergent dynamics in whole-body regeneration and larval development of sponge Haliclona simulans: cytobiology, microbiome, and transcriptomics. [Abstract]2026 Mar 13;29(4):115344. PMID: 41959672
Solvent & Solubility
DMSO : 233.33 mg/mL (1480.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (317.26 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (13.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (13.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (248 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 6.3452 mL | 31.7259 mL | 63.4518 mL | 158.6294 mL |
| 5 mM | 1.2690 mL | 6.3452 mL | 12.6904 mL | 31.7259 mL | |
| 10 mM | 0.6345 mL | 3.1726 mL | 6.3452 mL | 15.8629 mL | |
| 15 mM | 0.4230 mL | 2.1151 mL | 4.2301 mL | 10.5753 mL | |
| 20 mM | 0.3173 mL | 1.5863 mL | 3.1726 mL | 7.9315 mL | |
| 25 mM | 0.2538 mL | 1.2690 mL | 2.5381 mL | 6.3452 mL | |
| 30 mM | 0.2115 mL | 1.0575 mL | 2.1151 mL | 5.2876 mL | |
| 40 mM | 0.1586 mL | 0.7931 mL | 1.5863 mL | 3.9657 mL | |
| 50 mM | 0.1269 mL | 0.6345 mL | 1.2690 mL | 3.1726 mL | |
| 60 mM | 0.1058 mL | 0.5288 mL | 1.0575 mL | 2.6438 mL | |
| 80 mM | 0.0793 mL | 0.3966 mL | 0.7931 mL | 1.9829 mL | |
| 100 mM | 0.0635 mL | 0.3173 mL | 0.6345 mL | 1.5863 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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