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  4. HEPES

HEPES 

Cat. No.: HY-D0857 Purity: ≥98.0%
COA Handling Instructions

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.

For research use only. We do not sell to patients.

HEPES Chemical Structure

HEPES Chemical Structure

CAS No. : 7365-45-9

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of HEPES:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE HEPES

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  • Customer Review

Description

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis[1][2][3].

In Vitro

HEPES maintains superhydrophilicity of titanium for at least 3 months and resulted in a continuous retention of bioactivity and osteoconductivity[1].
HEPES drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, resulting the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

238.30

Appearance

Solid

Formula

C8H18N2O4S
CAS No.
SMILES

O=S(CCN1CCN(CCO)CC1)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 250 mg/mL (1049.10 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1964 mL 20.9820 mL 41.9639 mL
5 mM 0.8393 mL 4.1964 mL 8.3928 mL
10 mM 0.4196 mL 2.0982 mL 4.1964 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: ≥98.0%

COA (244 KB) HNMR (249 KB) MS (176 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

HEPES7365-45-9Biochemical Assay ReagentschemicalbufferingcellculturelysosomebiogenesiszwitterionicInhibitorinhibitorinhibit

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