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  3. Biochemical Assay Reagents
  4. HEPES

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.

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HEPES Chemical Structure

HEPES Chemical Structure

CAS No. : 7365-45-9

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1 g USD 70 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of HEPES:

HEPES Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis[1][2][3].

In Vitro

HEPES maintains superhydrophilicity of titanium for at least 3 months and resulted in a continuous retention of bioactivity and osteoconductivity[1].
HEPES drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, resulting the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HEPES Related Antibodies
Molecular Weight

238.30

Formula

C8H18N2O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCN1CCN(CCO)CC1)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 250 mg/mL (1049.10 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1964 mL 20.9820 mL 41.9639 mL
5 mM 0.8393 mL 4.1964 mL 8.3928 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (244 KB) HNMR (275 KB) MS (70 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.1964 mL 20.9820 mL 41.9639 mL 104.9098 mL
5 mM 0.8393 mL 4.1964 mL 8.3928 mL 20.9820 mL
10 mM 0.4196 mL 2.0982 mL 4.1964 mL 10.4910 mL
15 mM 0.2798 mL 1.3988 mL 2.7976 mL 6.9940 mL
20 mM 0.2098 mL 1.0491 mL 2.0982 mL 5.2455 mL
25 mM 0.1679 mL 0.8393 mL 1.6786 mL 4.1964 mL
30 mM 0.1399 mL 0.6994 mL 1.3988 mL 3.4970 mL
40 mM 0.1049 mL 0.5245 mL 1.0491 mL 2.6227 mL
50 mM 0.0839 mL 0.4196 mL 0.8393 mL 2.0982 mL
60 mM 0.0699 mL 0.3497 mL 0.6994 mL 1.7485 mL
80 mM 0.0525 mL 0.2623 mL 0.5245 mL 1.3114 mL
100 mM 0.0420 mL 0.2098 mL 0.4196 mL 1.0491 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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HEPES Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HEPES7365-45-9Biochemical Assay ReagentschemicalbufferingcellculturelysosomebiogenesiszwitterionicInhibitorinhibitorinhibit

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