1. Biochemical Assay Reagents
  2. HEPES

HEPES 

Cat. No.: HY-D0857 Purity: >98.0%
Handling Instructions

HEPES, a zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is a potent inducer of lysosome biogenesis.

For research use only. We do not sell to patients.

HEPES Chemical Structure

HEPES Chemical Structure

CAS No. : 7365-45-9

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10 mM * 1  mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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Description

HEPES, a zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is a potent inducer of lysosome biogenesis[1][2][3].

Molecular Weight

238.30

Formula

C₈H₁₈N₂O₄S

CAS No.

7365-45-9

SMILES

O=S(CCN1CCN(CCO)CC1)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 500 mg/mL (2098.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1964 mL 20.9820 mL 41.9639 mL
5 mM 0.8393 mL 4.1964 mL 8.3928 mL
10 mM 0.4196 mL 2.0982 mL 4.1964 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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  • Molarity Calculator

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

HEPESOtherschemicalbufferingcellculturelysosomebiogenesiszwitterionicInhibitorinhibitorinhibit

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HEPES
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