HEPES-d₁₈

Cat. No.: HY-D0857S Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

HEPES-d₁₈ is the deuterium labeled HEPES[1]. HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis[2][3][4].

For research use only. We do not sell to patients.

HEPES-d₁₈ Chemical Structure

CAS No. : 203805-87-2

Size	Price	Stock	Quantity
1 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Other Forms of HEPES-d₁₈:

HEPES In-stock

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

256.42

Formula

C₈D₁₈N₂O₄S

CAS No.

203805-87-2

Appearance

Solid

Color

White to off-white

SMILES

O=S(C([2H])([2H])C([2H])([2H])N1C([2H])([2H])C([2H])([2H])N(C([2H])([2H])C1([2H])[2H])C([2H])([2H])C([2H])([2H])O[2H])(O[2H])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (389.99 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8999 mL	19.4993 mL	38.9985 mL
5 mM	0.7800 mL	3.8999 mL	7.7997 mL
10 mM	0.3900 mL	1.9499 mL	3.8999 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (255 KB)

Handling Instructions (2659 KB)

References

[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216. [Content Brief]

[2]. Suzuki T, et al. Nonvolatile buffer coating of titanium to prevent its biological aging and for drug delivery. Biomaterials. 2010;31(18):4818-4828. [Content Brief]

[3]. Sledź P, et al. An experimental charge density of HEPES. Acta Crystallogr B. 201066(Pt 4):482-492. [Content Brief]

[4]. Tol MJ, et al. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution. Autophagy. 201814(3):437-449. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.8999 mL	19.4993 mL	38.9985 mL	97.4963 mL
	5 mM	0.7800 mL	3.8999 mL	7.7997 mL	19.4993 mL
	10 mM	0.3900 mL	1.9499 mL	3.8999 mL	9.7496 mL
	15 mM	0.2600 mL	1.3000 mL	2.5999 mL	6.4998 mL
	20 mM	0.1950 mL	0.9750 mL	1.9499 mL	4.8748 mL
	25 mM	0.1560 mL	0.7800 mL	1.5599 mL	3.8999 mL
	30 mM	0.1300 mL	0.6500 mL	1.3000 mL	3.2499 mL
	40 mM	0.0975 mL	0.4875 mL	0.9750 mL	2.4374 mL
	50 mM	0.0780 mL	0.3900 mL	0.7800 mL	1.9499 mL
	60 mM	0.0650 mL	0.3250 mL	0.6500 mL	1.6249 mL
	80 mM	0.0487 mL	0.2437 mL	0.4875 mL	1.2187 mL
	100 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9750 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

HEPES-d₁₈ Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HEPES-d₁₈203805-87-2Isotope-Labeled CompoundsInhibitorinhibitorinhibit

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HEPES-d18

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