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  4. Ethylenediaminetetraacetic acid

Ethylenediaminetetraacetic acid  (Synonyms: EDTA)

Cat. No.: HY-Y0682 Purity: 98.29%
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Ethylenediaminetetraacetic acid (EDTA) is a kind of metal chelating agent (binds to bivalent and trivalent metal cations, including calcium). Ethylenediaminetetraacetic acid has antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemia and anticoagulant activities. Ethylenediaminetetraacetic acid decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. Ethylenediaminetetraacetic acid can alleviate the liver fibrosis. Ethylenediaminetetraacetic acid can be used for coronary artery disease and neural system disease research.

For research use only. We do not sell to patients.

CAS No. : 60-00-4

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Ethylenediaminetetraacetic acid Related Antibodies

Top Publications Citing Use of Products

29 Publications Citing Use of MCE Ethylenediaminetetraacetic acid

IHC

    Ethylenediaminetetraacetic acid purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1660-1672.  [Abstract]

    Representative images of CHMP4–GFP puncta (arrowheads; left) and percentage of CHMP4 speckle+ (top right) or Annexin V+ (bottom right) cells in BMDMs incubated with PBS, 15 µM SK56, 2 mM EDTA or EDTA + SK56 at 2 h after the addition of 1 μg/ml LPS + 10 μM nigericin; scale bar, 50 µm; n = 5 repeats

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    Description

    Ethylenediaminetetraacetic acid (EDTA) is a kind of metal chelating agent (binds to bivalent and trivalent metal cations, including calcium). Ethylenediaminetetraacetic acid has antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemia and anticoagulant activities. Ethylenediaminetetraacetic acid decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. Ethylenediaminetetraacetic acid can alleviate the liver fibrosis. Ethylenediaminetetraacetic acid can be used for coronary artery disease and neural system disease research[1][2][3][4][5][6][7].

    In Vitro

    Ethylenediaminetetraacetic acid has a strong bactericidal effect on the cell wall of P. aeruginosa and a. faecalis[4].
    Ethylenediaminetetraacetic acid (0.005-0.01 M) has good heavy metal extraction in contaminated silty-clay-loam soil columns, can extract Pb, Cd and Zn in a concentration-dependent way with an extraction efficiency sequence of Pb > Cd > Zn[6].
    Ethylenediaminetetraacetic acid (1.2 mM) enhances the activity of the CRE driving promoter by activating T-cell death-associated gene 8 (TDAG8) in HEK293T cells, thereby enhancing the production of cAMP in the cells[7].
    When Ethylenediaminetetraacetic acid (EDTA) is prepared, NaOH is needed to adjust it in order to dissolve it. A relatively large amount of NaOH is required, and the dissolution is instantaneous, so NaOH needs to be added slowly, little by little.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ethylenediaminetetraacetic acid Related Antibodies
    In Vivo

    Ethylenediaminetetraacetic acid (60 mg/kg; Intraperitoneal injection; Three times per week for three weeks) can reduce liver fibrosis, lipid peroxidation and liver inflammation in CCl4 induced liver fibrosis rats, and has antioxidant and anti-inflammatory activities[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rat model of cirrhosis induced by CCl4[5]
    Dosage: 60 mg/kg, 120 mg/kg, 240 mg/kg
    Administration: Intraperitoneal injection (i.p.); Three times per week for 3 weeks (during this period, CCl4 administration continued). After CCl4 and mineral oil mixture treatment (200 μL/mouse; i.p.; Three times per week for eight weeks).
    Result: Kept kept all the rats alive at the 60 mg/kg dose, but died at 120 and 240 mg/kg.
    Reduced fibrosis of the liver in surviving rats (20%).
    Animal Model: Male Wistar rat model of cirrhosis induced by CCl[5]
    Dosage: 60 mg/kg
    Administration: Intraperitoneal injection (i.p.); Three times per week for 11 weeks. (Preventive Ethylenediaminetetraacetic acid (EDTA) group: EDTA and CCl4 were administered during 11 weeks, three times per week on alternate days).
    Intraperitoneal injection (i.p.); Three times per week. After CCl4 treatment (i.p.; Three times per week for eight weeks) (Therapeutic EDTA group: EDTA and CCl4 were administered for 3 weeks, three times per week on alternate days).
    Result: Increased sod activity by 50% in the preventive EDTA group. (Compared with untreated EDTA group)
    Increased Cp activity in the preventive EDTA (30%) and therapeutic EDTA (20%) groups. (Compared with the fibrotic group)
    Decreased mRNA expression of the pro-inflammatory molecule (TNF-α and LI-6) and the profibrogenic molecules (TGF-β and αCOLI) in both the prevention and treatment groups.
    Clinical Trial
    Molecular Weight

    292.24

    Formula

    C10H16N2O8
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    RT, protect from light, stored under nitrogen

    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    1M NaOH : 25 mg/mL (85.55 mM; Need ultrasonic)

    0.1 M NaOH : 6.67 mg/mL (22.82 mM; ultrasonic and warming and adjust pH to 7 with NaOH and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4218 mL 17.1092 mL 34.2185 mL
    5 mM 0.6844 mL 3.4218 mL 6.8437 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 98.29%

    SDS (393 KB)

    COA (247 KB) HNMR (123 KB) RP-HPLC (276 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    0.1 M NaOH / 1M NaOH 1 mM 3.4218 mL 17.1092 mL 34.2185 mL 85.5461 mL
    5 mM 0.6844 mL 3.4218 mL 6.8437 mL 17.1092 mL
    10 mM 0.3422 mL 1.7109 mL 3.4218 mL 8.5546 mL
    15 mM 0.2281 mL 1.1406 mL 2.2812 mL 5.7031 mL
    20 mM 0.1711 mL 0.8555 mL 1.7109 mL 4.2773 mL
    1M NaOH 25 mM 0.1369 mL 0.6844 mL 1.3687 mL 3.4218 mL
    30 mM 0.1141 mL 0.5703 mL 1.1406 mL 2.8515 mL
    40 mM 0.0855 mL 0.4277 mL 0.8555 mL 2.1387 mL
    50 mM 0.0684 mL 0.3422 mL 0.6844 mL 1.7109 mL
    60 mM 0.0570 mL 0.2852 mL 0.5703 mL 1.4258 mL
    80 mM 0.0428 mL 0.2139 mL 0.4277 mL 1.0693 mL
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    Ethylenediaminetetraacetic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ethylenediaminetetraacetic acid60-00-4EDTAEnvironmental PollutantsBiochemical Assay ReagentsBacterialSODEnvironmental ContaminantsSuperoxide DismutaseAnticoagulantAnti-hypercalcemicProtein purificationProtein storageHEK293TTDAG8cAMPChelating agentHypercalcemiaHepatic fibrosisInhibitorinhibitorinhibit

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