2. Anti-infection Immunology/Inflammation
  3. Bacterial SOD
  4. EDTA dihydrate tripotassium

EDTA dihydrate tripotassium  (Synonyms: Ethylenediaminetetraacetic acid dihydrate tripotassium)

Cat. No.: HY-W087947 Purity: 97.0%
COA
SDS
Handling Instructions Technical Support

EDTA dihydrate tripotassium (Ethylenediaminetetraacetic acid dihydrate tripotassium) is a kind of metal chelating agent (binds to bivalent and trivalent metal cations, including calcium). EDTA dihydrate tripotassium has antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemia and anticoagulant activities. EDTA dihydrate tripotassium decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. EDTA dihydrate tripotassium can alleviate the liver fibrosis. EDTA dihydrate tripotassium can be used for coronary artery disease and neural system disease research.

For research use only. We do not sell to patients.

CAS No. : 65501-24-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
25 g In-stock
50 g In-stock
> 100 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 24 publication(s) in Google Scholar

Other Forms of EDTA dihydrate tripotassium:

EDTA dihydrate tripotassium Related Antibodies

Top Publications Citing Use of Products

    EDTA dihydrate tripotassium purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1660-1672.  [Abstract]

    Representative images of CHMP4–GFP puncta (arrowheads; left) and percentage of CHMP4 speckle+ (top right) or Annexin V+ (bottom right) cells in BMDMs incubated with PBS, 15 µM SK56, 2 mM EDTA or EDTA + SK56 at 2 h after the addition of 1 μg/ml LPS + 10 μM nigericin; scale bar, 50 µm; n = 5 repeats

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    EDTA dihydrate tripotassium (Ethylenediaminetetraacetic acid dihydrate tripotassium) is a kind of metal chelating agent (binds to bivalent and trivalent metal cations, including calcium). EDTA dihydrate tripotassium has antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemia and anticoagulant activities. EDTA dihydrate tripotassium decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. EDTA dihydrate tripotassium can alleviate the liver fibrosis. EDTA dihydrate tripotassium can be used for coronary artery disease and neural system disease research[1][2][3][4][5][6][7].

    In Vitro

    EDTA dihydrate tripotassium has a strong bactericidal effect on the cell wall of P. aeruginosa and a. faecalis[4].
    EDTA dihydrate tripotassium (0.005-0.01 M) has good heavy metal extraction in contaminated silty-clay-loam soil columns, can extract Pb, Cd and Zn in a concentration-dependent way with an extraction efficiency sequence of Pb > Cd > Zn[6].
    EDTA dihydrate tripotassium (1.2 mM) enhances the activity of the CRE driving promoter by activating T-cell death-associated gene 8 (TDAG8) in HEK293T cells, thereby enhancing the production of cAMP in the cells[7].
    When EDTA dihydrate tripotassium is prepared, NaOH is needed to adjust it in order to dissolve it. A relatively large amount of NaOH is required, and the dissolution is instantaneous, so NaOH needs to be added slowly, little by little.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    EDTA dihydrate tripotassium Related Antibodies
    In Vivo

    EDTA dihydrate tripotassium (60 mg/kg; Intraperitoneal injection; Three times per week for three weeks) can reduce liver fibrosis, lipid peroxidation and liver inflammation in CCl4 induced liver fibrosis rats, and has antioxidant and anti-inflammatory activities[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rat model of cirrhosis induced by CCl4[5]
    Dosage: 60 mg/kg, 120 mg/kg, 240 mg/kg
    Administration: Intraperitoneal injection (i.p.); Three times per week for 3 weeks (during this period, CCl4 administration continued). After CCl4 and mineral oil mixture treatment (200 μL/mouse; i.p.; Three times per week for eight weeks).
    Result: Kept kept all the rats alive at the 60 mg/kg dose, but died at 120 and 240 mg/kg.
    Reduced fibrosis of the liver in surviving rats (20%).
    Animal Model: Male Wistar rat model of cirrhosis induced by CCl[5]
    Dosage: 60 mg/kg
    Administration: Intraperitoneal injection (i.p.); Three times per week for 11 weeks. (Preventive EDTA dihydrate tripotassium group: EDTA and CCl4 were administered during 11 weeks, three times per week on alternate days).
    Intraperitoneal injection (i.p.); Three times per week. After CCl4 treatment (i.p.; Three times per week for eight weeks) (Therapeutic EDTA group: EDTA and CCl4 were administered for 3 weeks, three times per week on alternate days).
    Result: Increased sod activity by 50% in the preventive EDTA group. (Compared with untreated EDTA group)
    Increased Cp activity in the preventive EDTA (30%) and therapeutic EDTA (20%) groups. (Compared with the fibrotic group)
    Decreased mRNA expression of the pro-inflammatory molecule (TNF-α and LI-6) and the profibrogenic molecules (TGF-β and αCOLI) in both the prevention and treatment groups.
    Molecular Weight

    442.54

    Formula

    C10H17K3N2O10
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(O)CN(CCN(CC(O[K])=O)CC(O[K])=O)CC(O[K])=O.O.O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (225.97 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2597 mL 11.2984 mL 22.5968 mL
    5 mM 0.4519 mL 2.2597 mL 4.5194 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.2597 mL 11.2984 mL 22.5968 mL 56.4921 mL
    5 mM 0.4519 mL 2.2597 mL 4.5194 mL 11.2984 mL
    10 mM 0.2260 mL 1.1298 mL 2.2597 mL 5.6492 mL
    15 mM 0.1506 mL 0.7532 mL 1.5065 mL 3.7661 mL
    20 mM 0.1130 mL 0.5649 mL 1.1298 mL 2.8246 mL
    25 mM 0.0904 mL 0.4519 mL 0.9039 mL 2.2597 mL
    30 mM 0.0753 mL 0.3766 mL 0.7532 mL 1.8831 mL
    40 mM 0.0565 mL 0.2825 mL 0.5649 mL 1.4123 mL
    50 mM 0.0452 mL 0.2260 mL 0.4519 mL 1.1298 mL
    60 mM 0.0377 mL 0.1883 mL 0.3766 mL 0.9415 mL
    80 mM 0.0282 mL 0.1412 mL 0.2825 mL 0.7062 mL
    100 mM 0.0226 mL 0.1130 mL 0.2260 mL 0.5649 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    EDTA dihydrate tripotassium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    EDTA dihydrate tripotassium65501-24-8Ethylenediaminetetraacetic acid dihydrate tripotassiumBacterialSODSuperoxide DismutaseAnticoagulantAnti-hypercalcemicProtein purificationProtein storageHEK293TTDAG8cAMPChelating agentHypercalcemiaHepatic fibrosisInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    EDTA dihydrate tripotassium
    Cat. No.:
    HY-W087947
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (557 KB) Français - FR (557 KB) Deutsch - DE (557 KB) Norwegian - NO (557 KB) Español - ES (557 KB) Swedish - SV (557 KB) Italian - IT (557 KB) Korean - KR (557 KB) Portuguese - PT (557 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.