Ethylenediaminetetraacetic acid disodium dihydrate
Based on 29 publication(s) in Google Scholar
Ethylenediaminetetraacetic acid (EDTA) disodium dehydrate is a kind of metal chelating agent (binds to bivalent and trivalent metal cations, including calcium). Ethylenediaminetetraacetic acid disodium dehydrate has antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemia and anticoagulant activities. Ethylenediaminetetraacetic acid disodium dehydrate decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. Ethylenediaminetetraacetic acid disodium dehydrate can alleviate the liver fibrosis. Ethylenediaminetetraacetic acid disodium dehydrate can be used for coronary artery disease and neural system disease research.
For research use only. We do not sell to patients.
- Purity : 99.9%
- CAS No.: 6381-92-6
- Formula: C10H18N2Na2O10
- Molecular Weight:372.24
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-B1659
Glycerol -
HY-D0861
EGTA -
HY-D0842
BSA Protein -
HY-100545
BAPTA-AM -
HY-D1005
Poloxamer 407 (F127) -
HY-15917
DL-Dithiothreitol -
HY-K0010
Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) -
HY-B2243D
Anhydrous sodium dihydrogen phosphate, for cell culture -
HY-Y0682
Ethylenediaminetetraacetic acid -
HY-D0857
HEPES -
HY-Y0682AR
Ethylenediaminetetraacetic acid disodium dihydrate (Standard)
Publications Citing Use of MedChemExpress (MCE) Ethylenediaminetetraacetic acid disodium dihydrate
More- Nat Immunol. 2025 Oct;26(10):1660-1672. [Abstract]
- J Extracell Vesicles. 2025 Sep;14(9):e70162. [Abstract]
- Bone Res. 2025 Mar 3;13(1):30. [Abstract]
- Neuron. 2025 Jul 29:S0896-6273(25)00509-4. [Abstract]
- J Nanobiotechnology. 2025 Mar 6;23(1):175. [Abstract]
- Nucleic Acids Res. 2024 Dec 11;52(22):14112-14132. [Abstract]
- Adv Sci (Weinh). 2026 May;13(25):e15362. [Abstract]
- Adv Sci (Weinh). 2025 Apr;12(13):e2415229. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(6):e2411261. [Abstract]
- Adv Sci (Weinh). 2023 Feb;10(4):e2204998. [Abstract]
- Cell Death Dis. 2026 Jun 30.
- Int J Biol Macromol. 2026 Jan;335(Pt 2):149182. [Abstract]
- Int J Biol Macromol. 2025 Jun;311(Pt 3):144068. [Abstract]
- Int J Biol Macromol. 2023 Jan 15:225:1182-1192. [Abstract]
- EMBO Mol Med. 2025 Oct 15. [Abstract]
- Colloids Surf B Biointerfaces. 2024 Feb:234:113680. [Abstract]
- Molecules. 2024 Jul 30;29(15):3590. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2026 Jan 24;1872(4):168169. [Abstract]
- Ann Med. 2025 Dec;57(1):2548045. [Abstract]
- Food Qual Saf. 2025 Aug 4.
- iScience. 2026 Jun 1;29(6):116200. [Abstract]
- J Orthop Surg Res. 2025 Apr 22;20(1):403. [Abstract]
- J Diabetes Investig. 2026 Jun 8. [Abstract]
- Breast Cancer (Dove Med Press). 2025 Dec 20:17:1265-1278. [Abstract]
- Cell Immunol. 2024 Jan-Feb:395-396:104781. [Abstract]
- Opt Mater Express. 2026 Jun 23;16(7):2151-2163.
- BMC Med Genomics. 2025 Aug 9;18(1):128. [Abstract]
- Biosci Biotechnol Biochem. 2026 Jun 23;90(7):877-885. [Abstract]
- bioRxiv. 2023 Sep 8.
Customer Validation & Images
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IHC
Biological Activity
Description
In Vitro
Ethylenediaminetetraacetic acid has a strong bactericidal effect on the cell wall of P. aeruginosa and a. faecalis[4].
Ethylenediaminetetraacetic acid (0.005-0.01 M) has good heavy metal extraction in contaminated silty-clay-loam soil columns, can extract Pb, Cd and Zn in a concentration-dependent way with an extraction efficiency sequence of Pb > Cd > Zn[6].
Ethylenediaminetetraacetic acid (1.2 mM) enhances the activity of the CRE driving promoter by activating T-cell death-associated gene 8 (TDAG8) in HEK293T cells, thereby enhancing the production of cAMP in the cells[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar rat model of cirrhosis induced by CCl4[5]
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Dosage:60 mg/kg, 120 mg/kg, 240 mg/kg
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Administration:Intraperitoneal injection (i.p.); Three times per week for 3 weeks (during this period, CCl4 administration continued). After CCl4 and mineral oil mixture treatment (200 μL/mouse; i.p.; Three times per week for eight weeks).
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Result:Kept kept all the rats alive at the 60 mg/kg dose, but died at 120 and 240 mg/kg.
Reduced fibrosis of the liver in surviving rats (20%).
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Animal Model:Male Wistar rat model of cirrhosis induced by CCl[5]
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Dosage:60 mg/kg
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Administration:Intraperitoneal injection (i.p.); Three times per week for 3 weeks (during this period, Intraperitoneal injection (i.p.); Three times per week for 11 weeks. (Preventive Ethylenediaminetetraacetic acid (EDTA) group: EDTA and CCl4 were administered during 11 weeks, three times per week on alternate days).
Intraperitoneal injection (i.p.); Three times per week. After CCl4 treatment (i.p.; Three times per week for eight weeks) (Therapeutic EDTA group: EDTA and CCl4 were administered for 3 weeks, three times per week on alternate days). -
Result:Increased sod activity by 50% in the preventive EDTA group. (Compared with untreated EDTA group)
Increased Cp activity in the preventive EDTA (30%) and therapeutic EDTA (20%) groups. (Compared with the fibrotic group)
Decreased mRNA expression of the pro-inflammatory molecule (TNF-α and LI-6) and the profibrogenic molecules (TGF-β and αCOLI) in both the prevention and treatment groups.
Chemical Information
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CAS No. 6381-92-6
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Appearance Solid
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Molecular Weight 372.24
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Formula C10H18N2Na2O10
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Color White to off-white
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SMILES
O=C(O)CN(CCN(CC(O)=O)CC(O[Na])=O)CC(O[Na])=O.O.O
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Synonyms
EDTA disodium dihydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (29)
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Journal Impact Factor
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Most Recent
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Nat Immunol
Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory response. [Abstract]2025 Oct;26(10):1660-1672. PMID: 40954252
Ethylenediaminetetraacetic acid disodium dihydrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1660-1672. [Abstract]
Representative images of CHMP4–GFP puncta (arrowheads; left) and percentage of CHMP4 speckle+ (top right) or Annexin V+ (bottom right) cells in BMDMs incubated with PBS, 15 µM SK56, 2 mM EDTA or EDTA + SK56 at 2 h after the addition of 1 μg/ml LPS + 10 μM nigericin; scale bar, 50 µm; n = 5 repeats
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J Extracell Vesicles
Targeted Blockage of Pathological Extracellular Vesicles and Particles From Fibroblast-Like Synoviocytes for Osteoarthritis Relief: Proteomic Analysis and Cellular Effect. [Abstract]2025 Sep;14(9):e70162. PMID: 40919882 -
Bone Res
Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus. [Abstract]2025 Mar 3;13(1):30. PMID: 40025004 -
Neuron
Social interaction in mice suppresses breast cancer progression via a corticoamygdala neural circuit. [Abstract]2025 Jul 29:S0896-6273(25)00509-4. PMID: 40752486 -
J Nanobiotechnology
2025 Mar 6;23(1):175. PMID: 40050923 -
Nucleic Acids Res
2024 Dec 11;52(22):14112-14132. PMID: 39673265 -
Adv Sci (Weinh)
Atrophic Skeletal Muscle-Derived Extracellular Vesicles Transfer miR-125a-5p to Inhibit Bone Formation in Osteoporosis during Aging. [Abstract]2026 May;13(25):e15362. PMID: 41747076 -
Adv Sci (Weinh)
A Novel tRF, HCETSR, Derived From tRNA-Glu/TTC, Inhibits HCC Malignancy by Regulating the SPBTN1-catenin Complex Axis. [Abstract]2025 Apr;12(13):e2415229. PMID: 39921434 -
Adv Sci (Weinh)
Hybrid Cell Membrane-Engineered Nanocarrier for Triple-Action Strategy to Address Pseudomonas aeruginosa Infection. [Abstract]2025 Feb;12(6):e2411261. PMID: 39721013 -
Adv Sci (Weinh)
Regulatory Fibroblast-Like Synoviocytes Cell Membrane Coated Nanoparticles: A Novel Targeted Therapy for Rheumatoid Arthritis. [Abstract]2023 Feb;10(4):e2204998. PMID: 36509660 -
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Int J Biol Macromol
Targeting glutaryl-CoA dehydrogenase-driven acetyl coenzyme A acetyltransferase 2 crotonylation dysregulates cholesterol metabolism in pancreatic cancer cells. [Abstract]2026 Jan;335(Pt 2):149182. PMID: 41285334 -
Int J Biol Macromol
FKBP5 inhibitor suppresses platelet activation and thrombosis by inhibiting IKBKE/PI3K/Rap1 pathway. [Abstract]2025 Jun;311(Pt 3):144068. PMID: 40345291 -
Int J Biol Macromol
pH-responsive magnetic Fe3O4/carboxymethyl chitosan/aminated lignosulfonate nanoparticles with uniform size for targeted drug loading. [Abstract]2023 Jan 15:225:1182-1192. PMID: 36423809 -
EMBO Mol Med
Isoginkgetin antagonizes ALS pathologies in its animal and patient iPSC models via PINK1-Parkin-dependent mitophagy. [Abstract]2025 Oct 15. PMID: 41094045 -
Colloids Surf B Biointerfaces
Platelet membrane-derived biomimetic microbubbles with enhanced targeting ability for the early detection of myocardial ischemia-reperfusion injury. [Abstract]2024 Feb:234:113680. PMID: 38101143 -
Molecules
Enhancing Functional Properties and Protein Structure of Almond Protein Isolate Using High-Power Ultrasound Treatment. [Abstract]2024 Jul 30;29(15):3590. PMID: 39124994 -
Biochim Biophys Acta Mol Basis Dis
Goosecoid promotes hepatic stellate cell epithelial-mesenchymal transition via transcriptional activation of serum- and glucocorticoid-induced protein kinase 1 in liver fibrosis. [Abstract]2026 Jan 24;1872(4):168169. PMID: 41581345 -
Ann Med
Multi-omics analysis and validation of autophagy-related diagnostic biomarker in osteoarthritis. [Abstract]2025 Dec;57(1):2548045. PMID: 40828304 -
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iScience
Inhibition of soluble epoxide hydrolase ameliorates renal injury in IgA nephropathy by restoring epoxyeicosatrienoic acids. [Abstract]2026 Jun 1;29(6):116200. PMID: 42256278 -
J Orthop Surg Res
Epimedin B protects against bone loss and inflammation in diabetic osteoporosis rats by regulating OPG/RANKL pathway. [Abstract]2025 Apr 22;20(1):403. PMID: 40264188 -
J Diabetes Investig
Hypoxia-preconditioned adipose-derived mesenchymal stem cells-derived exosomes transferring H19 obstruct neutrophil extracellular traps formation via HOXA5-mediated inactivation of TLR4/NF-κB/NLRP3 inflammatory signaling. [Abstract]2026 Jun 8. PMID: 42257551 -
Breast Cancer (Dove Med Press)
Dexmedetomidine Suppresses Mitochondrial Autophagy and Apoptosis While Promoting Proliferation in Breast Cancer Cells in vitro via PI3K/AKT Signaling. [Abstract]2025 Dec 20:17:1265-1278. PMID: 41466772 -
Cell Immunol
Dexamethasone alleviates pulmonary sarcoidosis by regulating the TGF-β/Smad3 signaling to promote Th17/Treg cell rebalance. [Abstract]2024 Jan-Feb:395-396:104781. PMID: 38159414 -
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BMC Med Genomics
Arsenic sulfide alleviates the progression of liver cancer malignancy by inhibiting the expression of TLCD1 in mice. [Abstract]2025 Aug 9;18(1):128. PMID: 40783540 -
Biosci Biotechnol Biochem
Effect of pirfenidone on the regulation of lymph angiogenesis by PLK1 signaling to inhibit metastasis of lung cancer in vivo. [Abstract]2026 Jun 23;90(7):877-885. PMID: 42012458 -
Solvent & Solubility
In Vitro:
H2O : 33.33 mg/mL (89.54 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
[1]. Chumanov RS, et al. Artifact-inducing enrichment of ethylenediaminetetraacetic acid and ethyleneglycoltetraacetic acid on anion exchange resins. Anal Biochem. 2011 May 1;412(1):34-9. [Content Brief]
[2]. Banfi G, et al. The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes. Clin Chem Lab Med. 2007;45(5):565-76. [Content Brief]
[3]. Ibad A, et al. Chelation therapy in the treatment of cardiovascular diseases. J Clin Lipidol. 2016 Jan-Feb;10(1):58-62. [Content Brief]
[4]. Gray GW, et al. The effect of ethylenediaminetetra-acetic acid on the cell walls of some gram-negative bacteria. J Gen Microbiol. 1965 Jun;39(3):385-99. [Content Brief]
[5]. González-Cuevas J, et al. Ethylenediaminetetraacetic acid induces antioxidant and anti-inflammatory activities in experimental liver fibrosis. Redox Rep. 2011;16(2):62-70. [Content Brief]
[7]. Deai M, et al. Ethylenediaminetetraacetic acid (EDTA) enhances cAMP production in human TDAG8-expressing cells. Biochem Biophys Res Commun. 2022 Oct 20;626:15-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.6864 mL | 13.4322 mL | 26.8644 mL | 67.1610 mL |
| 5 mM | 0.5373 mL | 2.6864 mL | 5.3729 mL | 13.4322 mL | |
| 10 mM | 0.2686 mL | 1.3432 mL | 2.6864 mL | 6.7161 mL | |
| 15 mM | 0.1791 mL | 0.8955 mL | 1.7910 mL | 4.4774 mL | |
| 20 mM | 0.1343 mL | 0.6716 mL | 1.3432 mL | 3.3580 mL | |
| 25 mM | 0.1075 mL | 0.5373 mL | 1.0746 mL | 2.6864 mL | |
| 30 mM | 0.0895 mL | 0.4477 mL | 0.8955 mL | 2.2387 mL | |
| 40 mM | 0.0672 mL | 0.3358 mL | 0.6716 mL | 1.6790 mL | |
| 50 mM | 0.0537 mL | 0.2686 mL | 0.5373 mL | 1.3432 mL | |
| 60 mM | 0.0448 mL | 0.2239 mL | 0.4477 mL | 1.1193 mL | |
| 80 mM | 0.0336 mL | 0.1679 mL | 0.3358 mL | 0.8395 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ethylenediaminetetraacetic acid disodium dihydrate Related Classifications
HY-Y0682A Related Classifications
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