1. Biochemical Assay Reagents
  2. EGTA

EGTA 

Cat. No.: HY-D0861
Handling Instructions

EGTA is a specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibited the substrate adherence capacity of inflammatory macrophages.

For research use only. We do not sell to patients.

EGTA Chemical Structure

EGTA Chemical Structure

CAS No. : 67-42-5

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Description

EGTA is a specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibited the substrate adherence capacity of inflammatory macrophages[1][2].

In Vitro

EGTA, proposed as endodontic irrigant, decreases substrate adherence capacity of inflammatory macrophages in a time- and dose-dependent manner. The EGTA concentration that causes an IC50 is 202 mM. Chelators react with calcium ions in the hydroxyapatite crystals to produce a metallic chelate. Removal of calcium ions from the dentine makes the dentinal tissue softer, especially the hydroxyapatite-rich peritubular dentin and increases the diameter of exposed dentinal tubules[2].

Molecular Weight

380.35

Formula

C₁₄H₂₄N₂O₁₀

CAS No.

67-42-5

SMILES

O=C(O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature

*In solvent : -80°C, 6 months; -20°C, 1 month (Store at room temperature)

Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (65.73 mM; ultrasonic and adjust pH to 10 with 1M NaOH)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6292 mL 13.1458 mL 26.2916 mL
5 mM 0.5258 mL 2.6292 mL 5.2583 mL
10 mM 0.2629 mL 1.3146 mL 2.6292 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

EGTAOtherscalciumionchelatorendodonticirrigantinflammatorymacrophagesInhibitorinhibitorinhibit

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