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  2. EGTA

EGTA 

Cat. No.: HY-D0861 Purity: >95.0%
Handling Instructions

EGTA is a specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibits the substrate adherence capacity of inflammatory macrophages.

For research use only. We do not sell to patients.

EGTA Chemical Structure

EGTA Chemical Structure

CAS No. : 67-42-5

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500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

EGTA is a specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibits the substrate adherence capacity of inflammatory macrophages[1][2].

In Vitro

EGTA, proposed as endodontic irrigant, decreases substrate adherence capacity of inflammatory macrophages in a time- and dose-dependent manner. The EGTA concentration that causes an IC50 is 202 mM. Chelators react with calcium ions in the hydroxyapatite crystals to produce a metallic chelate. Removal of calcium ions from the dentine makes the dentinal tissue softer, especially the hydroxyapatite-rich peritubular dentin and increases the diameter of exposed dentinal tubules[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

380.35

Formula

C₁₄H₂₄N₂O₁₀

CAS No.

67-42-5

SMILES

O=C(O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 10 mg/mL (26.29 mM; Need ultrasonic)

DMSO : 1 mg/mL (2.63 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6292 mL 13.1458 mL 26.2916 mL
5 mM 0.5258 mL 2.6292 mL 5.2583 mL
10 mM 0.2629 mL 1.3146 mL 2.6292 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

EGTAOtherscalciumionchelatorendodonticirrigantinflammatorymacrophagesInhibitorinhibitorinhibit

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EGTA
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HY-D0861
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