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  4. EGTA

EGTA is a cell-impermeant specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibits the substrate adherence capacity of inflammatory macrophages.

For research use only. We do not sell to patients.

CAS No. : 67-42-5

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Based on 25 publication(s) in Google Scholar

Other Forms of EGTA:

EGTA Related Antibodies

Top Publications Citing Use of Products
Flow Cytometry
Bio/Physico-chemical Assay
IF
2D/3D Cell Culture and Differentiation
WB
Cell Autophagy Assay
Cell Proliferation/Viability Assay
Cell Imaging/Staining

    EGTA purchased from MedChemExpress. Usage Cited in: mBio. 2024 Jun 3:e0119124.  [Abstract]

    HFFs were infected with Towne or Towne-ΔUL23 and co-cultured with or without activated Jurkat cells, followed by determination of viral titers. Prior to co-culture, the culture medium was sequentially supplemented with EGTA (5 mg/mL), Kp7-6 (10 mg/mL), anti-human IFN-γ neutralizing antibody (IFN-γ NAb, 10 µg/mL), anti-TRAIL neutralizing antibody (TRAIL NAb, 10 µg/mL), and infliximab (10 ng/mL) for 30 min.

    EGTA purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537.  [Abstract]

    EGTA (30 nM; 2 h). NLRP3/caspase-1/GSDMD protein levels (D) in BMDM cell by LPS + ATP.

    EGTA purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537.  [Abstract]

    Cell viability in BMDM cell by LPS + ATP. The results showed that EGTA (30 nM; 2 h) promoted cell viability.

    EGTA purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537.  [Abstract]

    Mitochondrion (Electron microscope) in BMDM cell by LPS + ATP. The results showed that EGTA (30 nM; 2 h) inhibited mitochondrial damage.

    EGTA purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Nov 28:577:216435.  [Abstract]

    Flow cytometric analysis of Annexin V-FITC/PI-PerCP-stained cell line Hut-102 were pretreated with CDN-1163 (50 μM), 2-APB (100 μM) or EGTA (5 mM) for 1 h, then exposed to 15 μM LW-213 for 6 h (n ≥ 3, ns means no significant difference, *p < 0.05, **p < 0.01 vs. 15 μM).

    EGTA purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Jun 4;19(9):2914-2933.  [Abstract]

    Ca2+ mobilization in FLAG-LcMtnr1b1-expressing HEK293 cells were measured in response to indicated concentrations of MT with pre-treated intracellular calcium chelator (BAPTA-AM, 50 μM), extracellular calcium chelator (EGTA, 5 mM; 30-300 min), or BAPTA-AM+EGTA, respectively.

    EGTA purchased from MedChemExpress. Usage Cited in: J Anim Sci Biotechnol. 2023 Dec 20;14(1):157.  [Abstract]

    MMEC was pretreated with calcium chelating agent EGTA (3 mM), and then added with S. maltophilia for 6 h before being collected. Calcium concentration was measured by Flou-4 fluorescence probe.

    EGTA purchased from MedChemExpress. Usage Cited in: J Anim Sci Biotechnol. 2023 Dec 20;14(1):157.  [Abstract]

    MMEC was pretreated with calcium chelating agent EGTA (3 mM), and then added with S. maltophilia for 6 h before being collected. The autophagic level of MMECs was significantly reduced by EGTA pretreatment.

    • Biological Activity

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    Description

    EGTA is a cell-impermeant specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibits the substrate adherence capacity of inflammatory macrophages[1][2].

    In Vitro

    EGTA, proposed as endodontic irrigant, decreases substrate adherence capacity of inflammatory macrophages in a time- and dose-dependent manner. The EGTA concentration that causes an IC50 is 202 mM. Chelators react with calcium ions in the hydroxyapatite crystals to produce a metallic chelate. Removal of calcium ions from the dentine makes the dentinal tissue softer, especially the hydroxyapatite-rich peritubular dentin and increases the diameter of exposed dentinal tubules[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    EGTA Related Antibodies
    Molecular Weight

    380.35

    Formula

    C14H24N2O10
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Store at room temperature 3 years

    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    1 M NaOH : ≥ 100 mg/mL (262.92 mM)

    H2O : 1 mg/mL (2.63 mM; ultrasonic and warming and heat to 60°C)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6292 mL 13.1458 mL 26.2916 mL
    5 mM 0.5258 mL 2.6292 mL 5.2583 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.89%

    SDS (396 KB)

    COA (244 KB) HNMR (130 KB) RP-HPLC (243 KB) MS (73 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / 1 M NaOH 1 mM 2.6292 mL 13.1458 mL 26.2916 mL 65.7289 mL
    1 M NaOH 5 mM 0.5258 mL 2.6292 mL 5.2583 mL 13.1458 mL
    10 mM 0.2629 mL 1.3146 mL 2.6292 mL 6.5729 mL
    15 mM 0.1753 mL 0.8764 mL 1.7528 mL 4.3819 mL
    20 mM 0.1315 mL 0.6573 mL 1.3146 mL 3.2864 mL
    25 mM 0.1052 mL 0.5258 mL 1.0517 mL 2.6292 mL
    30 mM 0.0876 mL 0.4382 mL 0.8764 mL 2.1910 mL
    40 mM 0.0657 mL 0.3286 mL 0.6573 mL 1.6432 mL
    50 mM 0.0526 mL 0.2629 mL 0.5258 mL 1.3146 mL
    60 mM 0.0438 mL 0.2191 mL 0.4382 mL 1.0955 mL
    80 mM 0.0329 mL 0.1643 mL 0.3286 mL 0.8216 mL
    100 mM 0.0263 mL 0.1315 mL 0.2629 mL 0.6573 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    EGTA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    EGTA67-42-5Biochemical Assay ReagentscalciumionchelatorendodonticirrigantinflammatorymacrophagesInhibitorinhibitorinhibit

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