EGTA tetrasodium

Name: EGTA tetrasodium
Brand: MedChemExpress
SKU: HY-D0861A
Rating: 5.0 (25 reviews)

Cat. No.: HY-D0861A Purity: 99.93%: Data Sheet
COA

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EGTA tetrasodium is a cell-impermeant specific calcium ion chelator. EGTA tetrasodium has an apparent calcium dissociation constant (K_d) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca²⁺ over Mg²⁺ (Mg²⁺ K_d 1-10 mM). EGTA tetrasodium significantly inhibits the substrate adherence capacity of inflammatory macrophages.

For research use only. We do not sell to patients.

CAS No. : 13368-13-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Based on 25 publication(s) in Google Scholar

Other Forms of EGTA tetrasodium:

EGTA In-stock

25 Publications Citing Use of MCE EGTA tetrasodium

Flow Cytometry

Bio/Physico-chemical Assay

2D/3D Cell Culture and Differentiation

Cell Autophagy Assay

Cell Proliferation/Viability Assay

Cell Imaging/Staining

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: mBio. 2024 Jun 3:e0119124. [Abstract]; HFFs were infected with Towne or Towne-ΔUL23 and co-cultured with or without activated Jurkat cells, followed by determination of viral titers. Prior to co-culture, the culture medium was sequentially supplemented with EGTA (5 mg/mL), Kp7-6 (10 mg/mL), anti-human IFN-γ neutralizing antibody (IFN-γ NAb, 10 µg/mL), anti-TRAIL neutralizing antibody (TRAIL NAb, 10 µg/mL), and infliximab (10 ng/mL) for 30 min.

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537. [Abstract]; EGTA (30 nM; 2 h). NLRP3/caspase-1/GSDMD protein levels (D) in BMDM cell by LPS + ATP.

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537. [Abstract]; Cell viability in BMDM cell by LPS + ATP. The results showed that EGTA (30 nM; 2 h) promoted cell viability.

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537. [Abstract]; Mitochondrion (Electron microscope) in BMDM cell by LPS + ATP. The results showed that EGTA (30 nM; 2 h) inhibited mitochondrial damage.

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Nov 28:577:216435. [Abstract]; Flow cytometric analysis of Annexin V-FITC/PI-PerCP-stained cell line Hut-102 were pretreated with CDN-1163 (50 μM), 2-APB (100 μM) or EGTA (5 mM) for 1 h, then exposed to 15 μM LW-213 for 6 h (n ≥ 3, ns means no significant difference, *p < 0.05, **p < 0.01 vs. 15 μM).

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Jun 4;19(9):2914-2933. [Abstract]; Ca²⁺ mobilization in FLAG-LcMtnr1b1-expressing HEK293 cells were measured in response to indicated concentrations of MT with pre-treated intracellular calcium chelator (BAPTA-AM, 50 μM), extracellular calcium chelator (EGTA, 5 mM; 30-300 min), or BAPTA-AM+EGTA, respectively.

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: J Anim Sci Biotechnol. 2023 Dec 20;14(1):157. [Abstract]; MMEC was pretreated with calcium chelating agent EGTA (3 mM), and then added with S. maltophilia for 6 h before being collected. Calcium concentration was measured by Flou-4 fluorescence probe.

: EGTA tetrasodium purchased from MedChemExpress. Usage Cited in: J Anim Sci Biotechnol. 2023 Dec 20;14(1):157. [Abstract]; MMEC was pretreated with calcium chelating agent EGTA (3 mM), and then added with S. maltophilia for 6 h before being collected. The autophagic level of MMECs was significantly reduced by EGTA pretreatment.

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Description

In Vitro

EGTA tetrasodium, proposed as endodontic irrigant, decreases substrate adherence capacity of inflammatory macrophages in a time- and dose-dependent manner. The EGTA tetrasodium concentration that causes an IC₅₀ is 202 mM. Chelators react with calcium ions in the hydroxyapatite crystals to produce a metallic chelate. Removal of calcium ions from the dentine makes the dentinal tissue softer, especially the hydroxyapatite-rich peritubular dentin and increases the diameter of exposed dentinal tubules^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

468.28

Formula

C₁₄H₂₀N₂Na₄O₁₀

CAS No.

13368-13-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[Na])CN(CC(O[Na])=O)CCOCCOCCN(CC(O[Na])=O)CC(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (213.55 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1355 mL	10.6774 mL	21.3547 mL
5 mM	0.4271 mL	2.1355 mL	4.2709 mL
10 mM	0.2135 mL	1.0677 mL	2.1355 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (271 KB) SDS (394 KB)

COA (246 KB) HNMR (249 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Harris RA, Hanrahan JW. Effects of EGTA on calcium signaling in airway epithelial cells. Am J Physiol. 1994;267(5 Pt 1):C1426-C1434. doi:10.1152/ajpcell.1994.267.5.C1426. [Content Brief]

[2]. Segura-Egea JJ, Jiménez-Rubio A, Rios-Santos JV, Velasco-Ortega E, Calvo-Gutierrez JR. In vitro inhibitory effect of EGTA on macrophage adhesion: endodontic implications. J Endod. 2003;29(3):211-213. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.1355 mL	10.6774 mL	21.3547 mL	53.3869 mL
	5 mM	0.4271 mL	2.1355 mL	4.2709 mL	10.6774 mL
	10 mM	0.2135 mL	1.0677 mL	2.1355 mL	5.3387 mL
	15 mM	0.1424 mL	0.7118 mL	1.4236 mL	3.5591 mL
	20 mM	0.1068 mL	0.5339 mL	1.0677 mL	2.6693 mL
	25 mM	0.0854 mL	0.4271 mL	0.8542 mL	2.1355 mL
	30 mM	0.0712 mL	0.3559 mL	0.7118 mL	1.7796 mL
	40 mM	0.0534 mL	0.2669 mL	0.5339 mL	1.3347 mL
	50 mM	0.0427 mL	0.2135 mL	0.4271 mL	1.0677 mL
	60 mM	0.0356 mL	0.1780 mL	0.3559 mL	0.8898 mL
	80 mM	0.0267 mL	0.1335 mL	0.2669 mL	0.6673 mL
	100 mM	0.0214 mL	0.1068 mL	0.2135 mL	0.5339 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.