EDTA dipotassium dihydrate
Based on 31 publication(s) in Google Scholar
EDTA dipotassium dihydrate is a metal ion chelator that forms stable and water-soluble chelates with a variety of metal ions. EDTA dipotassium is commonly used as a blood anticoagulant, which can effectively prevent blood coagulation and maintain the morphology and function of cells in blood samples. In the industrial field, EDTA dipotassium can be used to remove the adverse effects of metal ions and prevent oxidation and deterioration caused by metal ions.
For research use only. We do not sell to patients.
- Purity: 99.17%
- CAS No.: 25102-12-9
- Formula: C10H18K2N2O10
- Molecular Weight:404.45
-
Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) EDTA dipotassium dihydrate
More- Nat Immunol. 2025 Oct;26(10):1660-1672. [Abstract]
- J Extracell Vesicles. 2025 Sep;14(9):e70162. [Abstract]
- Bone Res. 2025 Mar 3;13(1):30. [Abstract]
- Neuron. 2025 Jul 29:S0896-6273(25)00509-4. [Abstract]
- J Nanobiotechnology. 2025 Mar 6;23(1):175. [Abstract]
- Nucleic Acids Res. 2024 Dec 11;52(22):14112-14132. [Abstract]
- Adv Sci (Weinh). 2026 May;13(25):e15362. [Abstract]
- Adv Sci (Weinh). 2025 Apr;12(13):e2415229. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(6):e2411261. [Abstract]
- Adv Sci (Weinh). 2023 Feb;10(4):e2204998. [Abstract]
- Int J Biol Macromol. 2026 Mar:352:151224. [Abstract]
- Int J Biol Macromol. 2026 Jan;335(Pt 2):149182. [Abstract]
- Int J Biol Macromol. 2025 Jun;311(Pt 3):144068. [Abstract]
- Int J Biol Macromol. 2023 Jan 15:225:1182-1192. [Abstract]
- EMBO Mol Med. 2025 Oct 15. [Abstract]
- Colloids Surf B Biointerfaces. 2024 Feb:234:113680. [Abstract]
- Molecules. 2024 Jul 30;29(15):3590. [Abstract]
- Molecules. 2021 Aug 24;26(17):5115. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2026 Jan 24;1872(4):168169. [Abstract]
- Ann Med. 2025 Dec;57(1):2548045. [Abstract]
- Food Qual Saf. 2025 Aug 4.
- iScience. 2026 Jun 1;29(6):116200. [Abstract]
- J Orthop Surg Res. 2025 Apr 22;20(1):403. [Abstract]
- J Diabetes Investig. 2026 Jun 8. [Abstract]
- Breast Cancer (Dove Med Press). 2025 Dec 20:17:1265-1278. [Abstract]
- Cell Immunol. 2024 Jan-Feb:395-396:104781. [Abstract]
- BMC Med Genomics. 2025 Aug 9;18(1):128. [Abstract]
- Biosci Biotechnol Biochem. 2026 Jun 23;90(7):877-885. [Abstract]
- bioRxiv. 2023 Sep 8.
- Research Square Preprint. 2021 Oct.
- Biomed Pharmacother. 2019 Jan:109:2427-2433. [Abstract]
Customer Validation & Images
-
IHC
All DNA/RNA Synthesis Isoforms
More
Biological Activity
EDTA dipotassium dihydrate can be used as a blood anticoagulant for hematological testing.
The drawn blood sample can be directly injected into a vacuum blood collection tube containing EDTA dipotassium dihydrate (2mL). Immediately after collection (<15 minutes) and 4 hours later, the sample can be tested for blood routine using a MEK-6318J/K hematology analyzer to measure multiple parameters including white blood cells, red blood cells, platelets, etc.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 25102-12-9
-
Appearance Solid
-
Molecular Weight 404.45
-
Formula C10H18K2N2O10
-
Color White to off-white
-
SMILES
O=C(O)CN(CCN(CC(O[K])=O)CC(O)=O)CC(O[K])=O.O.O
-
Synonyms
Ethylenediaminetetraacetic acid dipotassium dihydrate
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (31)
-
Journal Impact Factor
-
Most Recent
-
Nat Immunol
Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory response. [Abstract]2025 Oct;26(10):1660-1672. PMID: 40954252
EDTA dipotassium dihydrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1660-1672. [Abstract]
Representative images of CHMP4–GFP puncta (arrowheads; left) and percentage of CHMP4 speckle+ (top right) or Annexin V+ (bottom right) cells in BMDMs incubated with PBS, 15 µM SK56, 2 mM EDTA or EDTA + SK56 at 2 h after the addition of 1 μg/ml LPS + 10 μM nigericin; scale bar, 50 µm; n = 5 repeats
-
J Extracell Vesicles
Targeted Blockage of Pathological Extracellular Vesicles and Particles From Fibroblast-Like Synoviocytes for Osteoarthritis Relief: Proteomic Analysis and Cellular Effect. [Abstract]2025 Sep;14(9):e70162. PMID: 40919882 -
Bone Res
Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus. [Abstract]2025 Mar 3;13(1):30. PMID: 40025004 -
Neuron
Social interaction in mice suppresses breast cancer progression via a corticoamygdala neural circuit. [Abstract]2025 Jul 29:S0896-6273(25)00509-4. PMID: 40752486 -
J Nanobiotechnology
2025 Mar 6;23(1):175. PMID: 40050923 -
Nucleic Acids Res
2024 Dec 11;52(22):14112-14132. PMID: 39673265 -
Adv Sci (Weinh)
Atrophic Skeletal Muscle-Derived Extracellular Vesicles Transfer miR-125a-5p to Inhibit Bone Formation in Osteoporosis during Aging. [Abstract]2026 May;13(25):e15362. PMID: 41747076 -
Adv Sci (Weinh)
A Novel tRF, HCETSR, Derived From tRNA-Glu/TTC, Inhibits HCC Malignancy by Regulating the SPBTN1-catenin Complex Axis. [Abstract]2025 Apr;12(13):e2415229. PMID: 39921434 -
Adv Sci (Weinh)
Hybrid Cell Membrane-Engineered Nanocarrier for Triple-Action Strategy to Address Pseudomonas aeruginosa Infection. [Abstract]2025 Feb;12(6):e2411261. PMID: 39721013 -
Adv Sci (Weinh)
Regulatory Fibroblast-Like Synoviocytes Cell Membrane Coated Nanoparticles: A Novel Targeted Therapy for Rheumatoid Arthritis. [Abstract]2023 Feb;10(4):e2204998. PMID: 36509660 -
Int J Biol Macromol
Reticuline isomerase AKR1B1 with aldo-keto reductase activity and detoxification function from the insect Blaps rhynchopetera. [Abstract]2026 Mar:352:151224. PMID: 41791543 -
Int J Biol Macromol
Targeting glutaryl-CoA dehydrogenase-driven acetyl coenzyme A acetyltransferase 2 crotonylation dysregulates cholesterol metabolism in pancreatic cancer cells. [Abstract]2026 Jan;335(Pt 2):149182. PMID: 41285334 -
Int J Biol Macromol
FKBP5 inhibitor suppresses platelet activation and thrombosis by inhibiting IKBKE/PI3K/Rap1 pathway. [Abstract]2025 Jun;311(Pt 3):144068. PMID: 40345291 -
Int J Biol Macromol
pH-responsive magnetic Fe3O4/carboxymethyl chitosan/aminated lignosulfonate nanoparticles with uniform size for targeted drug loading. [Abstract]2023 Jan 15:225:1182-1192. PMID: 36423809 -
EMBO Mol Med
Isoginkgetin antagonizes ALS pathologies in its animal and patient iPSC models via PINK1-Parkin-dependent mitophagy. [Abstract]2025 Oct 15. PMID: 41094045 -
Colloids Surf B Biointerfaces
Platelet membrane-derived biomimetic microbubbles with enhanced targeting ability for the early detection of myocardial ischemia-reperfusion injury. [Abstract]2024 Feb:234:113680. PMID: 38101143 -
Molecules
Enhancing Functional Properties and Protein Structure of Almond Protein Isolate Using High-Power Ultrasound Treatment. [Abstract]2024 Jul 30;29(15):3590. PMID: 39124994 -
Molecules
Gadolinium-Based Paramagnetic Relaxation Enhancement Agent Enhances Sensitivity for NUS Multidimensional NMR-Based Metabolomics. [Abstract]2021 Aug 24;26(17):5115. PMID: 34500549 -
Biochim Biophys Acta Mol Basis Dis
Goosecoid promotes hepatic stellate cell epithelial-mesenchymal transition via transcriptional activation of serum- and glucocorticoid-induced protein kinase 1 in liver fibrosis. [Abstract]2026 Jan 24;1872(4):168169. PMID: 41581345 -
Ann Med
Multi-omics analysis and validation of autophagy-related diagnostic biomarker in osteoarthritis. [Abstract]2025 Dec;57(1):2548045. PMID: 40828304 -
-
iScience
Inhibition of soluble epoxide hydrolase ameliorates renal injury in IgA nephropathy by restoring epoxyeicosatrienoic acids. [Abstract]2026 Jun 1;29(6):116200. PMID: 42256278 -
J Orthop Surg Res
Epimedin B protects against bone loss and inflammation in diabetic osteoporosis rats by regulating OPG/RANKL pathway. [Abstract]2025 Apr 22;20(1):403. PMID: 40264188 -
J Diabetes Investig
Hypoxia-preconditioned adipose-derived mesenchymal stem cells-derived exosomes transferring H19 obstruct neutrophil extracellular traps formation via HOXA5-mediated inactivation of TLR4/NF-κB/NLRP3 inflammatory signaling. [Abstract]2026 Jun 8. PMID: 42257551 -
Breast Cancer (Dove Med Press)
Dexmedetomidine Suppresses Mitochondrial Autophagy and Apoptosis While Promoting Proliferation in Breast Cancer Cells in vitro via PI3K/AKT Signaling. [Abstract]2025 Dec 20:17:1265-1278. PMID: 41466772 -
Cell Immunol
Dexamethasone alleviates pulmonary sarcoidosis by regulating the TGF-β/Smad3 signaling to promote Th17/Treg cell rebalance. [Abstract]2024 Jan-Feb:395-396:104781. PMID: 38159414 -
BMC Med Genomics
Arsenic sulfide alleviates the progression of liver cancer malignancy by inhibiting the expression of TLCD1 in mice. [Abstract]2025 Aug 9;18(1):128. PMID: 40783540 -
Biosci Biotechnol Biochem
Effect of pirfenidone on the regulation of lymph angiogenesis by PLK1 signaling to inhibit metastasis of lung cancer in vivo. [Abstract]2026 Jun 23;90(7):877-885. PMID: 42012458 -
-
-
Biomed Pharmacother
2019 Jan:109:2427-2433. PMID: 30551502
Solvent & Solubility
H2O : ≥ 100 mg/mL (247.25 mM)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
-
Handling Instructions (2659 KB)
References
[1]. Heindorff K, et al. Genetic toxicology of ethylenediaminetetraacetic acid (EDTA) . Mutat Res. 1983 Jun;115 (2) :149-73. [Content Brief]
[2]. Mehmood R, et al. Evaluation of di-potassium and tri-potassium EDTA evacuated tubes for routine haematological testing. J Clin Lab Anal. 2018 Jan;32 (1) :e22188. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.4725 mL | 12.3625 mL | 24.7249 mL | 61.8123 mL |
| 5 mM | 0.4945 mL | 2.4725 mL | 4.9450 mL | 12.3625 mL | |
| 10 mM | 0.2472 mL | 1.2362 mL | 2.4725 mL | 6.1812 mL | |
| 15 mM | 0.1648 mL | 0.8242 mL | 1.6483 mL | 4.1208 mL | |
| 20 mM | 0.1236 mL | 0.6181 mL | 1.2362 mL | 3.0906 mL | |
| 25 mM | 0.0989 mL | 0.4945 mL | 0.9890 mL | 2.4725 mL | |
| 30 mM | 0.0824 mL | 0.4121 mL | 0.8242 mL | 2.0604 mL | |
| 40 mM | 0.0618 mL | 0.3091 mL | 0.6181 mL | 1.5453 mL | |
| 50 mM | 0.0494 mL | 0.2472 mL | 0.4945 mL | 1.2362 mL | |
| 60 mM | 0.0412 mL | 0.2060 mL | 0.4121 mL | 1.0302 mL | |
| 80 mM | 0.0309 mL | 0.1545 mL | 0.3091 mL | 0.7727 mL | |
| 100 mM | 0.0247 mL | 0.1236 mL | 0.2472 mL | 0.6181 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
EDTA dipotassium dihydrate Related Classifications
HY-D0836 Related Classifications
Powered by Bioz