DNase I, Bovine pancreas
Based on 41 publication(s) in Google Scholar
DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer.
For research use only. We do not sell to patients.
- CAS No.: 9003-98-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-129046
RNase A, Bovine pancreas
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HY-E70005D
Collagenase IV, Clostridium histolytica
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HY-E70005A
Collagenase, Type I
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HY-18739
Phorbol 12-myristate 13-acetate
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HY-107910
Hyaluronidase, Bovine testes
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HY-10071
Y-27632
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HY-E70005B
Collagenase, Type II
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HY-108882A
Recombinant DNase I (RNase-free)
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HY-108717
Proteinase K
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HY-108882C
DNase I (filtered)
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HY-131577
Neutral protease, Paenibacillus polymyxa
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HY-P1645
Papain
Publications Citing Use of MedChemExpress (MCE) DNase I, Bovine pancreas
More- J Hematol Oncol. 2024 Dec 18;17(1):126. [Abstract]
- Adv Funct Mater. 2025 Sep 2.
- Nat Commun. 2025 Dec 29. [Abstract]
- ACS Nano. 2026 Mar 31;20(12):10108-10126. [Abstract]
- Redox Biol. 2026 Jun 27:95:104280. [Abstract]
- Acta Pharm Sin B. 2026 Feb 13.
- Adv Sci (Weinh). 2026 Apr;13(20):e15431. [Abstract]
- Adv Sci (Weinh). 2026 Mar 17:e16199. [Abstract]
- Adv Sci (Weinh). 2025 Dec 17:e19334. [Abstract]
- Adv Sci (Weinh). 2025 Apr;12(16):e2411524. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(5):e2408323. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(5):e2410495. [Abstract]
- Sci Adv. 2026 Jun 19;12(25):eaec1031. [Abstract]
- Research (Wash D C). 2025 May 30:8:0705. [Abstract]
- Chem Eng J. 2025 Oct 15;524:169690.
- J Control Release. 2026 Jul 10:395:115004. [Abstract]
- J Control Release. 2025 Oct 14;388(Pt 1):114337. [Abstract]
- J Control Release. 2025 Apr 10:380:1109-1126. [Abstract]
- Mater Today Bio. 2026 Mar 31:38:103086. [Abstract]
- Mater Today Bio. 2025 Mar 27:32:101712. [Abstract]
- Cell Death Discov. 2024 Sep 5;10(1):395. [Abstract]
- Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
- J Orthop Translat. 2026 Feb 11:56:101032. [Abstract]
- Int J Pharm X. 2026 Mar 26:11:100528. [Abstract]
- Front Immunol. 2026 Jan 13:16:1712786. [Abstract]
- J Agric Food Chem. 2025 Aug 13;73(32):20396-20409. [Abstract]
- Ecotoxicol Environ Saf. 2024 Aug:281:116678. [Abstract]
- Commun Biol. 2026 Jun 1. [Abstract]
- Chem Biol Interact. 2026 Jul 1:434:112120. [Abstract]
- Mol Pharm. 2026 Jun 1;23(6):3276-3286. [Abstract]
- Sci Rep. 2024 Jul 16;14(1):16457. [Abstract]
- FASEB J. 2026 May 15;40(9):e71847. [Abstract]
- Mol Carcinog. 2024 Sep;63(9):1827-1841. [Abstract]
- Biochim Biophys Acta Gen Subj. 2026 Sep;1870(9):130969. [Abstract]
- Vet Sci. 2024 Dec 12;11(12):643. [Abstract]
- Cancer Manag Res. 2026 Jun 16:18:600327. [Abstract]
- Biochem Biophys Res Commun. 2024 Dec 20:739:150914. [Abstract]
- Arq Bras Cardiol. 2024 Oct;121(10):e20240181. [Abstract]
- bioRxiv. 2026 May 29.
- bioRxiv. 2026 Apr 28.
- Biomed Pharmacother. 2025 Sep 18:192:118565. [Abstract]
Customer Validation & Images
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Histological Imaging/Staining
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IF
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WB
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Cell Imaging/Staining
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Cell Migration/Invasion Assay
Biological Activity
Product Information The activity of DNase Ⅰ is dependent on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, Zn2+, and others. In the presence of Mg2+, the enzyme can randomly recognize and cleave any site on either strand of DNA; whereas, in the presence of Mn2+, it can simultaneously recognize both strands of DNA and cleave at nearly identical sites. This product is extracted from bovine pancreas and operates optimally within a pH range of 7-8. It has a molecular weight of approximately 31 kDa and an isoelectric point of around 6.0. Instructions Inactivation or Inhibition of DNase I: Dissolved DNase I can be inactivated by heating at 65°C for 10 min. Phenol-chloroform extraction can also inactivate DNase I. Metal ion chelators, zinc ions at millimolar concentrations, 0.1% SDS, reducing agents such as DTT and β-mercaptoethanol, and salt concentrations above 50-100 mM all significantly inhibit DNase I. Usage Method for Protein Extraction Experiments (for reference only): 1)Preparation of storage solution: 5-10 mg/mL dissolved in 0.15 M NaCl, stored in the refrigerator, it is recommended to be used within 1 week. 2)Reaction System: Add DNase I stock solution to the protein extraction buffer at a 1/100 volume ratio (to achieve a final concentration of 20 U/mL) and 1 M MgCl2 at a 1/100 volume ratio. 3)Reaction Conditions: Incubate at 37°C for 30-60 min. Proceed with subsequent protein extraction experiments. Notes 1.Once the solution is prepared, please store it in aliquots to avoid product failure caused by repeated freezing and thawing. 2.Since EDTA can chelate Ca2+ and Mg2+ required for enzyme activity, EDTA should be removed from the initial protein lysis buffer, as it will reduce the digestive capacity of DNase I.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c x DBA/2 mice with murine tumour L5178Y-ML cells subcutaneous injection[1]
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Dosage:0.1 U per mouse
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Administration:Intravenous injection; 0.1 U; once daily for three days
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Result:Reduced liver weight from 2.26 to 1.55 g, affected the intensity of liver metastasis, reduced the numbers and sizes of metastatic and affected tumour cell arrest.
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Animal Model:Female BALB/c x DBA/2 mice with murine tumour and surgical removal of subcutaneous tumours[1]
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Dosage:0.1 U per mouse
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Administration:Intravenous injection; 0.1 U; once daily for 3 days either before or after primary tumour removal
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Result:Significantly prolongated survival after tumour cell inoculation compared to the untreated group.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
3.1.21.1
>2000 Kunitz U/mg
One Kunitz unit is defined as the amount of enzyme that causes a change in absorbance of 0.001 at 260 nm (ΔA₂₆₀) per minute per mL when using Type I DNA as substrate under the conditions of pH 5.0 and 37°C
Technical Parameters
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Biological Source
Bovine pancreas
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pH Stability
pH 7-8
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Thermal Stability
Below 65℃
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Activators
Ca2+、Mg2+
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Inhibitors
0.1% SDS, DTT, mercaptoethanol and other reducing agents
Chemical Information
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CAS No. 9003-98-9
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[DNase I, Bovine pancreas]
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Synonyms
DNase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (41)
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Journal Impact Factor
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Most Recent
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J Hematol Oncol
myCAF-derived exosomal PWAR6 accelerates CRC liver metastasis via altering glutamine availability and NK cell function in the tumor microenvironment. [Abstract]2024 Dec 18;17(1):126. PMID: 39696364 -
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Nat Commun
IL-17a induces age-related olfactory dysfunction by impairing regeneration and promoting respiratory metaplasia in mice. [Abstract]2025 Dec 29. PMID: 41461651 -
ACS Nano
Cellular Adaptive Component-Mediated Targeting of Chylomicron-Mimic Nanoemulsion to Hepatic Stellate Cells for Liver Fibrosis. [Abstract]2026 Mar 31;20(12):10108-10126. PMID: 41830798 -
Redox Biol
Histidine metabolic reprogramming drives oxidative stress induced mtDNA release to promote necroptosis and airway inflammation in severe asthma. [Abstract]2026 Jun 27:95:104280. PMID: 42372708 -
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Adv Sci (Weinh)
pH/ROS-Responsive Injectable Hydrogel Co-Loaded with B7-H3 Blocker and NETs Suppressor Boosts OSCC Synergistic Immunotherapy. [Abstract]2026 Apr;13(20):e15431. PMID: 41684277 -
Adv Sci (Weinh)
CRP Deficiency Rescues Periodontitis-Induced Hippocampal Neurogenesis Impairment by Suppressing OPC-Derived BMP4 Signaling in Rats. [Abstract]2026 Mar 17:e16199. PMID: 41841594 -
Adv Sci (Weinh)
An Enzyme-Like Catalyzed Nanosheets for Redox Stress Oscillation Therapy Against Bacterial Infections. [Abstract]2025 Dec 17:e19334. PMID: 41405368 -
Adv Sci (Weinh)
2025 Apr;12(16):e2411524. PMID: 40013981 -
Adv Sci (Weinh)
Sequential Release HydroLipo System for STING Gene Epigenetic Reprogramming and Immune Activation in Glioblastoma. [Abstract]2025 Feb;12(5):e2408323. PMID: 39661716 -
Adv Sci (Weinh)
Bacteroides Fragilis Exacerbates T2D Vascular Calcification by Secreting Extracellular Vesicles to Induce M2 Macrophages. [Abstract]2025 Feb;12(5):e2410495. PMID: 39665119 -
Sci Adv
Aptamer-functionalized apoptotic vesicles ameliorate osteoarthritis via resuming mitochondria OXPHOS of chondrocytes. [Abstract]2026 Jun 19;12(25):eaec1031. PMID: 42308286 -
Research (Wash D C)
Lactate-Induced Mitochondrial Calcium Uptake 3 Aggravates Myocardial Ischemia-Reperfusion Injury by Promoting Neutrophil Extracellular Trap Formation. [Abstract]2025 May 30:8:0705. PMID: 40452820
DNase I, Bovine pancreas purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]
Masson staining was conducted to detect fibrosis in tissue samples from different groups. Sham: 3.29% ± 0.64%, MIRI: 30.28% ± 5.87%, MIRI + DNase I: 15.91% ±2.92%, MIRI + GSK484: 17.12% ± 2.48%.
DNase I, Bovine pancreas purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]
IF was performed to detect the expression of VE-cadherin of HUVECs under different treatments. Neutrophils/Sham: 136.08 ± 20.98, Neutrophils/MIRI: 69.45 ± 8.91, Neutrophils/MIRI + DNase I: 108.14 ± 12.38, Neutrophils/MIRI + GSK484: 111.44 ± 16.16.
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J Control Release
2026 Jul 10:395:115004. PMID: 42128062 -
J Control Release
2025 Oct 14;388(Pt 1):114337. PMID: 41101699 -
J Control Release
Archaea-inspired deoxyribonuclease I liposomes prevent multiple organ dysfunction in sepsis. [Abstract]2025 Apr 10:380:1109-1126. PMID: 39986474 -
Mater Today Bio
Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity. [Abstract]2026 Mar 31:38:103086. PMID: 42006712 -
Mater Today Bio
Metal-organic framework-based delivery systems as nanovaccine for enhancing immunity against porcine circovirus type 2. [Abstract]2025 Mar 27:32:101712. PMID: 40230641 -
Cell Death Discov
Highly sensitive magnetic particle imaging of abdominal aortic aneurysm NETosis with anti-Ly6G iron oxide nanoparticles. [Abstract]2024 Sep 5;10(1):395. PMID: 39237520
DNase I, Bovine pancreas purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Sep 5;10(1):395. [Abstract]
The whole aorta was detected by western blot, and the expressionof NETs products (ELANE, MPO) was quantitatively analyzed treatd with DNase I (4 mg/kg, intraperitoneally daily for 3 weeks).
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Cell Death Discov
Neutrophil extracellular traps promote invasion and metastasis via NLRP3-mediated oral squamous cell carcinoma pyroptosis inhibition. [Abstract]2024 May 2;10(1):214. PMID: 38697992
DNase I, Bovine pancreas purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
The effect of NETs on the proliferation of Cal27 cells was detected by EdU proliferation assay DNase I (1 μg/mL,37°C, 15 min).
DNase I, Bovine pancreas purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
The effect of NETs on the invasion of Cal27 cells was detected by Transwell invasion assay DNase I (1 μg/mL,37°C, 15 min).
DNase I, Bovine pancreas purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
The effect of NETs on the migration of Cal27 cells was detected by wound healing assay treated with DNase I (1 μg/mL,37°C, 15 min).
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J Orthop Translat
Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury. [Abstract]2026 Feb 11:56:101032. PMID: 41836554 -
Int J Pharm X
Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis. [Abstract]2026 Mar 26:11:100528. PMID: 41952672 -
Front Immunol
2026 Jan 13:16:1712786. PMID: 41607775 -
J Agric Food Chem
Neurotoxic Domino Effect: Dietary Silica Nanoparticles Spark Microglial Extracellular Traps and Neuronal Pyroptosis. [Abstract]2025 Aug 13;73(32):20396-20409. PMID: 40740003 -
Ecotoxicol Environ Saf
The role of neutrophil extracellular traps in β-methylamino L-alanine-induced liver injury in mice. [Abstract]2024 Aug:281:116678. PMID: 38964067 -
Commun Biol
CBX4 enhances acute monocytic leukemia development via HDAC-mediated suppression of Runx1. [Abstract]2026 Jun 1. PMID: 42225948 -
Chem Biol Interact
Polystyrene nanoplastics induce hippocampal damage and cognitive deficits through oxidative stress-triggered microglial extracellular traps and neuronal ferroptosis. [Abstract]2026 Jul 1:434:112120. PMID: 42070744 -
Mol Pharm
Insect-Cell-Produced AAV2 Vectors Activate Myeloid Innate Immune Response Featuring NETosis. [Abstract]2026 Jun 1;23(6):3276-3286. PMID: 42157557 -
Sci Rep
Neutrophil extracellular traps promote erectile dysfunction in rats with diabetes mellitus by enhancing NLRP3-mediated pyroptosis. [Abstract]2024 Jul 16;14(1):16457. PMID: 39014129 -
FASEB J
M2 Macrophage-Derived Osteopontin Promotes Osteogenesis of Fibro-Adipogenic Progenitors After Spinal Cord Injury. [Abstract]2026 May 15;40(9):e71847. PMID: 42047733 -
Mol Carcinog
LAIR1 promotes hepatocellular carcinoma cell metastasis and induces M2-macrophage infiltration through activating AKT-IKKβ-p65 axis. [Abstract]2024 Sep;63(9):1827-1841. PMID: 39016636 -
Biochim Biophys Acta Gen Subj
DLGAP5-induced glycolysis reprogramming reinforces macrophage M2 polarization to impact BC progression. [Abstract]2026 Sep;1870(9):130969. PMID: 42297309 -
Vet Sci
Porcine Epidemic Diarrhea Virus Infection of Porcine Intestinal Epithelial Cells Causes Mitochondrial DNA Release and the Activation of the NLRP3 Inflammasome to Mediate Interleukin-1β Secretion. [Abstract]2024 Dec 12;11(12):643. PMID: 39728983 -
Cancer Manag Res
Berberine from Tibetan Medicine Dracocephalum tanguticum Maxim Suppresses Colorectal Tumor Growth and Inhibits CD8⁺ T Cells Ferroptosis via the NRF2-SLC7A11-GPX4 Axis. [Abstract]2026 Jun 16:18:600327. PMID: 42326541 -
Biochem Biophys Res Commun
2024 Dec 20:739:150914. PMID: 39536412 -
Arq Bras Cardiol
LncRNA CCAT2 Knockdown Alleviates Pressure Overload or Ang II-Induced Cardiac Hypertrophy Via Disruption of the Wnt/β-Catenin Signaling. [Abstract]2024 Oct;121(10):e20240181. PMID: 39536197 -
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Biomed Pharmacother
TME-responsive nanoparticles co-targeting VCP, NETs, and dual immune checkpoints for immune revitalization in EGFR/PD-L1/CTLA-4-driven colorectal cancer. [Abstract]2025 Sep 18:192:118565. PMID: 40972397
Solvent & Solubility
H2O : ≥ 4 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Lauková L, et al. Deoxyribonucleases and Their Applications in Biomedicine. Biomolecules. 2020 Jul 11;10(7):1036. [Content Brief]
[2]. Sugihara S, et al. Deoxyribonuclease treatment prevents blood-borne liver metastasis of cutaneously transplanted tumour cells in mice. Br J Cancer. 1993 Jan;67(1):66-70. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DNase I, Bovine pancreas Related Classifications
HY-108882 Related Classifications
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