DNase I RNase & Protease free

Cat. No.: HY-108882B: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DNase I is an enzyme that degrades DNA. DNase I is mainly produced by digestive system organs, such as the pancreas and parotid gland. Three types of DNase I are known in mammals: pancreatic type, parotid type, and pancreatico-parotid type. DNase I plays a key role in the cleavage of extracellular DNA, and is critical for limiting inflammatory responses and maintaining homeostasis. DNase I is responsible for digesting extracellular nucleoproteins, which may be essential for preventing autoimmune responses. Decreased DNase I activity may be associated with the onset and progression of systemic lupus erythematosus (SLE). DNase I (RNase & Protease free) is a molecular biology-grade DNase I purified by chromatography to remove RNase and protease.

For research use only. We do not sell to patients.

CAS No. : 9003-98-9

Size	Price	Stock	Quantity
2.5 KU		In-stock	Estimated Time of Arrival: December 31
10 KU		In-stock	Estimated Time of Arrival: December 31

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 29 publication(s) in Google Scholar

Other Forms of DNase I RNase & Protease free:

DNase I, Bovine pancreas In-stock
Recombinant DNase I (RNase-free) In-stock

29 Publications Citing Use of MCE DNase I RNase & Protease free

Histological Imaging/Staining

Cell Imaging/Staining

Cell Migration/Invasion Assay

: DNase I RNase & Protease free purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]; Masson staining was conducted to detect fibrosis in tissue samples from different groups. Sham: 3.29% ± 0.64%, MIRI: 30.28% ± 5.87%, MIRI + DNase I: 15.91% ±2.92%, MIRI + GSK484: 17.12% ± 2.48%.

: DNase I RNase & Protease free purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]; IF was performed to detect the expression of VE-cadherin of HUVECs under different treatments. Neutrophils/Sham: 136.08 ± 20.98, Neutrophils/MIRI: 69.45 ± 8.91, Neutrophils/MIRI + DNase I: 108.14 ± 12.38, Neutrophils/MIRI + GSK484: 111.44 ± 16.16.

: DNase I RNase & Protease free purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Sep 5;10(1):395. [Abstract]; The whole aorta was detected by western blot, and the expressionof NETs products (ELANE, MPO) was quantitatively analyzed treatd with DNase I (4 mg/kg, intraperitoneally daily for 3 weeks).

: DNase I RNase & Protease free purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]; The effect of NETs on the proliferation of Cal27 cells was detected by EdU proliferation assay DNase I (1 μg/mL,37°C, 15 min).

: DNase I RNase & Protease free purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]; The effect of NETs on the invasion of Cal27 cells was detected by Transwell invasion assay DNase I (1 μg/mL,37°C, 15 min).

: DNase I RNase & Protease free purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]; The effect of NETs on the migration of Cal27 cells was detected by wound healing assay treated with DNase I (1 μg/mL,37°C, 15 min).

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

View All Isoforms

RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

Biological Activity
Purity & Documentation
References
Customer Review

Description	DNase I is an enzyme that degrades DNA. DNase I is mainly produced by digestive system organs, such as the pancreas and parotid gland. Three types of DNase I are known in mammals: pancreatic type, parotid type, and pancreatico-parotid type. DNase I plays a key role in the cleavage of extracellular DNA, and is critical for limiting inflammatory responses and maintaining homeostasis. DNase I is responsible for digesting extracellular nucleoproteins, which may be essential for preventing autoimmune responses. Decreased DNase I activity may be associated with the onset and progression of systemic lupus erythematosus (SLE). DNase I (RNase & Protease free) is a molecular biology-grade DNase I purified by chromatography to remove RNase and protease^[1].
In Vitro	As a member of the DNase I family, DNase I cleaves DNA to generate two products with 5′-phosphate and 3′-hydroxyl termini, respectively^[1]. DNase I cleaves double-stranded DNA (dsDNA) 100-500 times more efficiently than single-stranded DNA (ssDNA), while the structure and sequence of DNA substrates affect hydrolysis kinetics. Compared to the relatively hydrolysis-resistant Z-DNA, B-DNA is the most suitable substrate for DNase I^[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. DNase I RNase & Protease free Related Antibodies
CAS No.	9003-98-9
Appearance	Solid
Color	White to off-white
SMILES	[DNase I (RNase & Protease free)]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Select Batch: Data Sheet (267 KB) SDS (251 KB) COA (242 KB) Handling Instructions (2659 KB)
References	[1]. Lauková L, et al. Deoxyribonucleases and Their Applications in Biomedicine. Biomolecules. 2020;10(7):1036. Published 2020 Jul 11. [Content Brief]