Recombinant DNase I (RNase-free)
Based on 40 publication(s) in Google Scholar
Recombinant DNase I (RNase-free) is a recombinant deoxyribonuclease that degrades DNA. Recombinant DNase I is essential for limiting inflammatory responses and maintaining homeostasis.
For research use only. We do not sell to patients.
- CAS No.: 9003-98-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Recombinant DNase I (RNase-free)
More- J Hematol Oncol. 2024 Dec 18;17(1):126. [Abstract]
- Adv Funct Mater. 2025 Sep 2.
- Nat Commun. 2025 Dec 29. [Abstract]
- ACS Nano. 2026 Mar 31;20(12):10108-10126. [Abstract]
- Redox Biol. 2026 Jun 27:95:104280. [Abstract]
- Acta Pharm Sin B. 2026 Feb 13.
- Adv Sci (Weinh). 2026 Apr;13(20):e15431. [Abstract]
- Adv Sci (Weinh). 2026 Mar 17:e16199. [Abstract]
- Adv Sci (Weinh). 2025 Dec 17:e19334. [Abstract]
- Adv Sci (Weinh). 2025 Apr;12(16):e2411524. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(5):e2410495. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(5):e2408323. [Abstract]
- Sci Adv. 2026 Jun 19;12(25):eaec1031. [Abstract]
- Research (Wash D C). 2025 May 30:8:0705. [Abstract]
- Chem Eng J. 2025 Oct 15;524:169690.
- J Control Release. 2026 Jul 10:395:115004. [Abstract]
- J Control Release. 2025 Oct 14;388(Pt 1):114337. [Abstract]
- J Control Release. 2025 Apr 10:380:1109-1126. [Abstract]
- Mater Today Bio. 2026 Mar 31:38:103086. [Abstract]
- Mater Today Bio. 2025 Mar 27:32:101712. [Abstract]
- Cell Death Discov. 2024 Sep 5;10(1):395. [Abstract]
- Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
- J Orthop Translat. 2026 Feb 11:56:101032. [Abstract]
- Int J Pharm X. 2026 Mar 26:11:100528. [Abstract]
- Front Immunol. 2026 Jan 13:16:1712786. [Abstract]
- J Agric Food Chem. 2025 Aug 13;73(32):20396-20409. [Abstract]
- Ecotoxicol Environ Saf. 2024 Aug:281:116678. [Abstract]
- Commun Biol. 2026 Jun 1. [Abstract]
- Chem Biol Interact. 2026 Jul 1:434:112120. [Abstract]
- Sci Rep. 2024 Jul 16;14(1):16457. [Abstract]
- FASEB J. 2026 May 15;40(9):e71847. [Abstract]
- Mol Carcinog. 2024 Sep;63(9):1827-1841. [Abstract]
- Biochim Biophys Acta Gen Subj. 2026 Sep;1870(9):130969. [Abstract]
- Vet Sci. 2024 Dec 12;11(12):643. [Abstract]
- Cancer Manag Res. 2026 Jun 16:18:600327. [Abstract]
- Biochem Biophys Res Commun. 2024 Dec 20:739:150914. [Abstract]
- Arq Bras Cardiol. 2024 Oct;121(10):e20240181. [Abstract]
- bioRxiv. 2026 May 29.
- bioRxiv. 2026 Apr 28.
- Biomed Pharmacother. 2025 Sep 18:192:118565. [Abstract]
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Histological Imaging/Staining
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IF
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WB
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Cell Imaging/Staining
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Cell Migration/Invasion Assay
Biological Activity
Product Information
DNase I is a non-specific endonuclease that degrades double-stranded and single-stranded DNA as well as chromosomes. It functions by hydrolyzing phosphodiester bonds to generate mononucleotides and oligonucleotides with 5'-phosphate and 3'-hydroxyl groups. The enzyme activity requires the presence of Mg2+ or Ca2+ ions, with an optimal pH of approximately 7.8. This product is recombinant DNase I derived from bovine pancreas and expressed in Pichia pastoris, with RNase activity removed during purification. The storage buffer consists of 10 mM Tris, 2 mM CaCl₂, 50% glycerol, pH 7.6
Removal of DNA Contamination from RNA
1. The reaction system contains: 10 μg RNA, 10 μL of 10× DNase I Reaction Buffer (100 mM Tris, 5 mM CaCl₂, 25 mM MgCl₂, pH 7.6), 2 U of recombinant DNase I, and water to make up the final volume to 100 μL.
2. Incubate at 37 ℃ for 10 min. After the reaction is complete, add EDTA to a final concentration of 5 mM and incubate at 75 ℃ for 10 min to inactivate DNase I.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.1.21.1
≥ 2 U/μL
One unit is defined as the amount of enzyme required to completely degrade 1 μg of plasmid DNA in 10min at 37°C
Chemical Information
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CAS No. 9003-98-9
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Recombinant DNase I (RNase-free)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (40)
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Journal Impact Factor
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Most Recent
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J Hematol Oncol
myCAF-derived exosomal PWAR6 accelerates CRC liver metastasis via altering glutamine availability and NK cell function in the tumor microenvironment. [Abstract]2024 Dec 18;17(1):126. PMID: 39696364 -
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Nat Commun
IL-17a induces age-related olfactory dysfunction by impairing regeneration and promoting respiratory metaplasia in mice. [Abstract]2025 Dec 29. PMID: 41461651 -
ACS Nano
Cellular Adaptive Component-Mediated Targeting of Chylomicron-Mimic Nanoemulsion to Hepatic Stellate Cells for Liver Fibrosis. [Abstract]2026 Mar 31;20(12):10108-10126. PMID: 41830798 -
Redox Biol
Histidine metabolic reprogramming drives oxidative stress induced mtDNA release to promote necroptosis and airway inflammation in severe asthma. [Abstract]2026 Jun 27:95:104280. PMID: 42372708 -
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Adv Sci (Weinh)
pH/ROS-Responsive Injectable Hydrogel Co-Loaded with B7-H3 Blocker and NETs Suppressor Boosts OSCC Synergistic Immunotherapy. [Abstract]2026 Apr;13(20):e15431. PMID: 41684277 -
Adv Sci (Weinh)
CRP Deficiency Rescues Periodontitis-Induced Hippocampal Neurogenesis Impairment by Suppressing OPC-Derived BMP4 Signaling in Rats. [Abstract]2026 Mar 17:e16199. PMID: 41841594 -
Adv Sci (Weinh)
An Enzyme-Like Catalyzed Nanosheets for Redox Stress Oscillation Therapy Against Bacterial Infections. [Abstract]2025 Dec 17:e19334. PMID: 41405368 -
Adv Sci (Weinh)
2025 Apr;12(16):e2411524. PMID: 40013981 -
Adv Sci (Weinh)
Bacteroides Fragilis Exacerbates T2D Vascular Calcification by Secreting Extracellular Vesicles to Induce M2 Macrophages. [Abstract]2025 Feb;12(5):e2410495. PMID: 39665119 -
Adv Sci (Weinh)
Sequential Release HydroLipo System for STING Gene Epigenetic Reprogramming and Immune Activation in Glioblastoma. [Abstract]2025 Feb;12(5):e2408323. PMID: 39661716 -
Sci Adv
Aptamer-functionalized apoptotic vesicles ameliorate osteoarthritis via resuming mitochondria OXPHOS of chondrocytes. [Abstract]2026 Jun 19;12(25):eaec1031. PMID: 42308286 -
Research (Wash D C)
Lactate-Induced Mitochondrial Calcium Uptake 3 Aggravates Myocardial Ischemia-Reperfusion Injury by Promoting Neutrophil Extracellular Trap Formation. [Abstract]2025 May 30:8:0705. PMID: 40452820
Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]
Masson staining was conducted to detect fibrosis in tissue samples from different groups. Sham: 3.29% ± 0.64%, MIRI: 30.28% ± 5.87%, MIRI + DNase I: 15.91% ±2.92%, MIRI + GSK484: 17.12% ± 2.48%.
Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]
IF was performed to detect the expression of VE-cadherin of HUVECs under different treatments. Neutrophils/Sham: 136.08 ± 20.98, Neutrophils/MIRI: 69.45 ± 8.91, Neutrophils/MIRI + DNase I: 108.14 ± 12.38, Neutrophils/MIRI + GSK484: 111.44 ± 16.16.
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J Control Release
2026 Jul 10:395:115004. PMID: 42128062 -
J Control Release
2025 Oct 14;388(Pt 1):114337. PMID: 41101699 -
J Control Release
Archaea-inspired deoxyribonuclease I liposomes prevent multiple organ dysfunction in sepsis. [Abstract]2025 Apr 10:380:1109-1126. PMID: 39986474 -
Mater Today Bio
Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity. [Abstract]2026 Mar 31:38:103086. PMID: 42006712 -
Mater Today Bio
Metal-organic framework-based delivery systems as nanovaccine for enhancing immunity against porcine circovirus type 2. [Abstract]2025 Mar 27:32:101712. PMID: 40230641 -
Cell Death Discov
Highly sensitive magnetic particle imaging of abdominal aortic aneurysm NETosis with anti-Ly6G iron oxide nanoparticles. [Abstract]2024 Sep 5;10(1):395. PMID: 39237520
Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Sep 5;10(1):395. [Abstract]
The whole aorta was detected by western blot, and the expressionof NETs products (ELANE, MPO) was quantitatively analyzed treatd with DNase I (4 mg/kg, intraperitoneally daily for 3 weeks).
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Cell Death Discov
Neutrophil extracellular traps promote invasion and metastasis via NLRP3-mediated oral squamous cell carcinoma pyroptosis inhibition. [Abstract]2024 May 2;10(1):214. PMID: 38697992
Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
The effect of NETs on the proliferation of Cal27 cells was detected by EdU proliferation assay DNase I (1 μg/mL,37°C, 15 min).
Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
The effect of NETs on the invasion of Cal27 cells was detected by Transwell invasion assay DNase I (1 μg/mL,37°C, 15 min).
Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]
The effect of NETs on the migration of Cal27 cells was detected by wound healing assay treated with DNase I (1 μg/mL,37°C, 15 min).
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J Orthop Translat
Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury. [Abstract]2026 Feb 11:56:101032. PMID: 41836554 -
Int J Pharm X
Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis. [Abstract]2026 Mar 26:11:100528. PMID: 41952672 -
Front Immunol
2026 Jan 13:16:1712786. PMID: 41607775 -
J Agric Food Chem
Neurotoxic Domino Effect: Dietary Silica Nanoparticles Spark Microglial Extracellular Traps and Neuronal Pyroptosis. [Abstract]2025 Aug 13;73(32):20396-20409. PMID: 40740003 -
Ecotoxicol Environ Saf
The role of neutrophil extracellular traps in β-methylamino L-alanine-induced liver injury in mice. [Abstract]2024 Aug:281:116678. PMID: 38964067 -
Commun Biol
CBX4 enhances acute monocytic leukemia development via HDAC-mediated suppression of Runx1. [Abstract]2026 Jun 1. PMID: 42225948 -
Chem Biol Interact
Polystyrene nanoplastics induce hippocampal damage and cognitive deficits through oxidative stress-triggered microglial extracellular traps and neuronal ferroptosis. [Abstract]2026 Jul 1:434:112120. PMID: 42070744 -
Sci Rep
Neutrophil extracellular traps promote erectile dysfunction in rats with diabetes mellitus by enhancing NLRP3-mediated pyroptosis. [Abstract]2024 Jul 16;14(1):16457. PMID: 39014129 -
FASEB J
M2 Macrophage-Derived Osteopontin Promotes Osteogenesis of Fibro-Adipogenic Progenitors After Spinal Cord Injury. [Abstract]2026 May 15;40(9):e71847. PMID: 42047733 -
Mol Carcinog
LAIR1 promotes hepatocellular carcinoma cell metastasis and induces M2-macrophage infiltration through activating AKT-IKKβ-p65 axis. [Abstract]2024 Sep;63(9):1827-1841. PMID: 39016636 -
Biochim Biophys Acta Gen Subj
DLGAP5-induced glycolysis reprogramming reinforces macrophage M2 polarization to impact BC progression. [Abstract]2026 Sep;1870(9):130969. PMID: 42297309 -
Vet Sci
Porcine Epidemic Diarrhea Virus Infection of Porcine Intestinal Epithelial Cells Causes Mitochondrial DNA Release and the Activation of the NLRP3 Inflammasome to Mediate Interleukin-1β Secretion. [Abstract]2024 Dec 12;11(12):643. PMID: 39728983 -
Cancer Manag Res
Berberine from Tibetan Medicine Dracocephalum tanguticum Maxim Suppresses Colorectal Tumor Growth and Inhibits CD8⁺ T Cells Ferroptosis via the NRF2-SLC7A11-GPX4 Axis. [Abstract]2026 Jun 16:18:600327. PMID: 42326541 -
Biochem Biophys Res Commun
2024 Dec 20:739:150914. PMID: 39536412 -
Arq Bras Cardiol
LncRNA CCAT2 Knockdown Alleviates Pressure Overload or Ang II-Induced Cardiac Hypertrophy Via Disruption of the Wnt/β-Catenin Signaling. [Abstract]2024 Oct;121(10):e20240181. PMID: 39536197 -
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Biomed Pharmacother
TME-responsive nanoparticles co-targeting VCP, NETs, and dual immune checkpoints for immune revitalization in EGFR/PD-L1/CTLA-4-driven colorectal cancer. [Abstract]2025 Sep 18:192:118565. PMID: 40972397
Purity & Documentation
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Data Sheet (268 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Lauková L, et al. Deoxyribonucleases and Their Applications in Biomedicine. Biomolecules. 2020 Jul 11;10(7):1036. [Content Brief]
[2]. Sugihara S, et al. Deoxyribonuclease treatment prevents blood-borne liver metastasis of cutaneously transplanted tumour cells in mice. Br J Cancer. 1993 Jan;67(1):66-70. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)