1. Enzyme
  2. Nuclease
  3. RNase A, bovine pancreas

RNase A, bovine pancreas  (Synonyms: Ribonuclease A; EC 3.1.27.5; RNase A)

Cat. No.: HY-129046
COA Handling Instructions

Ribonuclease A (EC 3.1.27.5) cleaves RNA 3′ to pyrimidines and actively cleaves RNA at every pyrimidine residue. Ribonuclease A catalyzes the hydrolysis of single stranded RNA in the absence of metal ions or cofactors.

For research use only. We do not sell to patients.

RNase A, bovine pancreas Chemical Structure

RNase A, bovine pancreas Chemical Structure

CAS No. : 9001-99-4

Size Price Stock Quantity
25 mg USD 30 In-stock
50 mg USD 50 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ribonuclease A (EC 3.1.27.5) cleaves RNA 3′ to pyrimidines and actively cleaves RNA at every pyrimidine residue. Ribonuclease A catalyzes the hydrolysis of single stranded RNA in the absence of metal ions or cofactors[1][2][3].

In Vitro

A major application of RNase A is the removal of RNA from plasmid DNA preparations.

Preparation of RNase A stock solution
1. Use 10 mM sodium acetate (pH 5.2) to prepare 10 mg/mL RNase A stock solution;
2. Heat at 100 ℃ for 15 min;
3. Cool to room temperature, add 1/10(v/v) of 1 M Tris-HCl(pH 7.4), adjust the pH to 7.4;
4. The solution can be stored at 20 ℃ for 2 years.
Note: When the RNase A solution is boiled under neutral conditions, RNase precipitate will form; if it is boiled at a lower pH, any precipitation may be caused by the presence of protein impurities. At this time, the precipitate can be removed by high-speed centrifugation (13 000 rpm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[RNase A, bovine pancreas]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (Need ultrasonic)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

RNase A, bovine pancreas Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
RNase A, bovine pancreas
Cat. No.:
HY-129046
Quantity:
MCE Japan Authorized Agent: