RNase B, Bovine Pancreas  (Synonyms: Ribonuclease B, Bovine Pancreas)

RNase A (Bovine pancreatic RNase) is a widely used Endonuclease in DNA purification by specifically hydrolyzing cytosine or uracil residues of RNA. RNase A degrades the RNA in the RNA/DNA duplex. RNase A catalyses the breakdown of 3',5'-phosphodiester linkages of single stranded RNA. RNase A family members in organisms are tightly involved in various physiological and pathological processes including cell growth and development, proliferation, differentiation and migration. Dysregulation of RNase A activity or expression level is closely related to pancreatic, ovarian, bladder and thyroid cancer. RNase A has tumor cell-killing ability. RNase B, Bovine Pancreas (Ribonuclease B, Bovine Pancreas) is the N-glycosylated form of RNase A. RNase B, Bovine Pancreas can promote the folding of polypeptide chains and play a role similar to molecular chaperones^[1][2][3].

RNase A (Bovine pancreatic RNase) is a widely used Endonuclease in DNA purification by specifically hydrolyzing cytosine or uracil residues of RNA. RNase A degrades the RNA in the RNA/DNA duplex. RNase A catalyses the breakdown of 3',5'-phosphodiester linkages of single stranded RNA. RNase A family members in organisms are tightly involved in various physiological and pathological processes including cell growth and development, proliferation, differentiation and migration. Dysregulation of RNase A activity or expression level is closely related to pancreatic, ovarian, bladder and thyroid cancer. RNase A has tumor cell-killing ability. RNase B, Bovine Pancreas (Ribonuclease B, Bovine Pancreas) is the N-glycosylated form of RNase A. RNase B, Bovine Pancreas can promote the folding of polypeptide chains and play a role similar to molecular chaperones^[1][2][3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solid

White to off-white

[RNase B, Bovine Pancreas]

≥50 Kunitz U/mg soild

One unit is defined as the amount of enzyme that will decrease the absorbance from A0 to Af in 1 min at 25°C and pH 5.0. A0 to Af corresponds to the total conversion and Af is the final absorbance.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Dang W, et al. RNase A activity analysis and imaging using label-free DNA-templated silver nanoclusters. Talanta. 2020 Mar 1;209:120512.  [Content Brief]

  [2]. Nogués MV, et al. Bovine pancreatic ribonuclease A as a model of an enzyme with multiple substrate binding sites. Biochim Biophys Acta. 1995 Nov 15;1253(1):16-24.  [Content Brief]

  [3]. Yamaguchi H, et al., A chaperone-like function of intramolecular high-mannose chains in the oxidative refolding of bovine pancreatic RNase B. J Biochem. 1996 Sep;120(3):474-7.  [Content Brief]