Bis-tris propane
Based on 1 Customer Validation
Bis-tris propane (BTP) is a water-soluble buffer substance. Bis-tris propane can be used as a suitable buffer for polymerase chain reaction (PCR). Bis-tris propane can enhance the stability or activity of restriction enzymes.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 64431-96-5
- Formula: C11H26N2O6
- Molecular Weight:282.33
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Bis-tris propane buffer exhibits high fidelity in PCR reactions by by stabilizing the pH of the reaction system, and reducing errors introduced by DNA polymerase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 64431-96-5
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Appearance Solid
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Molecular Weight 282.33
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Formula C11H26N2O6
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Color White to off-white
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SMILES
OCC(NCCCNC(CO)(CO)CO)(CO)CO
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Synonyms
BTP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 100 mg/mL (354.20 mM; Need ultrasonic)
DMSO : 25 mg/mL (88.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. K A Eckert, et al. DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl. 1991 Aug;1(1):17-24. [Content Brief]
[2]. J R Wenner, et al. Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage. Anal Biochem. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.5420 mL | 17.7098 mL | 35.4195 mL | 88.5489 mL |
| 5 mM | 0.7084 mL | 3.5420 mL | 7.0839 mL | 17.7098 mL | |
| 10 mM | 0.3542 mL | 1.7710 mL | 3.5420 mL | 8.8549 mL | |
| 15 mM | 0.2361 mL | 1.1807 mL | 2.3613 mL | 5.9033 mL | |
| 20 mM | 0.1771 mL | 0.8855 mL | 1.7710 mL | 4.4274 mL | |
| 25 mM | 0.1417 mL | 0.7084 mL | 1.4168 mL | 3.5420 mL | |
| 30 mM | 0.1181 mL | 0.5903 mL | 1.1807 mL | 2.9516 mL | |
| 40 mM | 0.0885 mL | 0.4427 mL | 0.8855 mL | 2.2137 mL | |
| 50 mM | 0.0708 mL | 0.3542 mL | 0.7084 mL | 1.7710 mL | |
| 60 mM | 0.0590 mL | 0.2952 mL | 0.5903 mL | 1.4758 mL | |
| 80 mM | 0.0443 mL | 0.2214 mL | 0.4427 mL | 1.1069 mL | |
| H2O | 100 mM | 0.0354 mL | 0.1771 mL | 0.3542 mL | 0.8855 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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