Bis-tris propane (Synonyms: BTP)

Name: Bis-tris propane
Brand: MedChemExpress
SKU: HY-126399
Rating: 4.5 (1 reviews)

Cat. No.: HY-126399 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bis-tris propane (BTP) is a water-soluble buffer substance. Bis-tris propane can be used as a suitable buffer for polymerase chain reaction (PCR). Bis-tris propane can enhance the stability or activity of restriction enzymes.

For research use only. We do not sell to patients.

Bis-tris propane Chemical Structure

CAS No. : 64431-96-5

Size	Price	Stock	Quantity

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Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	USD 30	In-stock	Estimated Time of Arrival: December 31
5 g	USD 40	In-stock	Estimated Time of Arrival: December 31
10 g	USD 50	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Molecular Weight

282.33

Formula

C₁₁H₂₆N₂O₆

CAS No.

64431-96-5

Appearance

Solid

Color

White to off-white

SMILES

OCC(NCCCNC(CO)(CO)CO)(CO)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (354.20 mM; Need ultrasonic)

DMSO : 25 mg/mL (88.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5420 mL	17.7098 mL	35.4195 mL
5 mM	0.7084 mL	3.5420 mL	7.0839 mL
10 mM	0.3542 mL	1.7710 mL	3.5420 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (245 KB) HNMR (261 KB)

Handling Instructions (2659 KB)

References

[1]. K A Eckert, et al. DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl. 1991 Aug;1(1):17-24. [Content Brief]

[2]. J R Wenner, et al. Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage. Anal Biochem. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.5420 mL	17.7098 mL	35.4195 mL	88.5489 mL
	5 mM	0.7084 mL	3.5420 mL	7.0839 mL	17.7098 mL
	10 mM	0.3542 mL	1.7710 mL	3.5420 mL	8.8549 mL
	15 mM	0.2361 mL	1.1807 mL	2.3613 mL	5.9033 mL
	20 mM	0.1771 mL	0.8855 mL	1.7710 mL	4.4274 mL
	25 mM	0.1417 mL	0.7084 mL	1.4168 mL	3.5420 mL
	30 mM	0.1181 mL	0.5903 mL	1.1807 mL	2.9516 mL
	40 mM	0.0885 mL	0.4427 mL	0.8855 mL	2.2137 mL
	50 mM	0.0708 mL	0.3542 mL	0.7084 mL	1.7710 mL
	60 mM	0.0590 mL	0.2952 mL	0.5903 mL	1.4758 mL
	80 mM	0.0443 mL	0.2214 mL	0.4427 mL	1.1069 mL
H₂O	100 mM	0.0354 mL	0.1771 mL	0.3542 mL	0.8855 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bis-tris propane Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bis-tris propane64431-96-5BTPBiochemical Assay Reagentspolymerase chain reactionPCRrestriction enzymeInhibitorinhibitorinhibit

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Bis-tris propane (Synonyms: BTP)

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Bis-tris propane Related Antibodies

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Complete Stock Solution Preparation Table

Bis-tris propane Related Classifications

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