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  4. THAM hydrochloride (≥99%, for cell culture)

THAM hydrochloride (≥99%, for cell culture)  (Synonyms: Tris HCl (≥99%, for cell culture); Tris hydrochloride (≥99%, for cell culture))

Cat. No.: HY-D0227J Purity: 99.67%
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THAM hydrochloride (≥99%, for cell culture) is a low-toxicity amino alcohol buffer, a specific CO2-consuming proton acceptor that buffers carbon dioxide and acid both in vitro and in vivo. THAM hydrochloride (≥99%, for cell culture) binds protons to form bicarbonate, reduces PaCO2, and induces intracellular alkalization, thereby ameliorating hypercapnia-induced elevation of pulmonary blood vessels and pulmonary arterial pressure. THAM hydrochloride (≥99%, for cell culture) may cause PaCO2 rebound, hypoglycemia, and respiratory depression. THAM hydrochloride (≥99%, for cell culture) removes amniotic epithelium and preserves the basement membrane, but depletes extracellular matrix and reduces the adhesion rate of limbal epithelial cells. THAM hydrochloride (≥99%, for cell culture) can act as a CO2 carrier to enhance the productivity and carbon utilization rate of Scenedesmus obliquus. THAM hydrochloride (≥99%, for cell culture) is a key component of buffer solutions used in various biological, cell culture, biochemical, and molecular biology applications.

For research use only. We do not sell to patients.

THAM hydrochloride (≥99%, for cell culture)

THAM hydrochloride (≥99%, for cell culture) Chemical Structure

CAS No. : 1185-53-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
25 g In-stock
50 g In-stock
100 g In-stock
500 g In-stock
1 kg In-stock

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Based on 1 publication(s) in Google Scholar

Other Forms of THAM hydrochloride (≥99%, for cell culture):

THAM hydrochloride (≥99%, for cell culture) Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE THAM hydrochloride (≥99%, for cell culture)

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

THAM hydrochloride (≥99%, for cell culture) is a low-toxicity amino alcohol buffer, a specific CO2-consuming proton acceptor that buffers carbon dioxide and acid both in vitro and in vivo. THAM hydrochloride (≥99%, for cell culture) binds protons to form bicarbonate, reduces PaCO2, and induces intracellular alkalization, thereby ameliorating hypercapnia-induced elevation of pulmonary blood vessels and pulmonary arterial pressure. THAM hydrochloride (≥99%, for cell culture) may cause PaCO2 rebound, hypoglycemia, and respiratory depression. THAM hydrochloride (≥99%, for cell culture) removes amniotic epithelium and preserves the basement membrane, but depletes extracellular matrix and reduces the adhesion rate of limbal epithelial cells. THAM hydrochloride (≥99%, for cell culture) can act as a CO2 carrier to enhance the productivity and carbon utilization rate of Scenedesmus obliquus. THAM hydrochloride (≥99%, for cell culture) is a key component of buffer solutions used in various biological, cell culture, biochemical, and molecular biology applications[1][2][3][4][5].

In Vitro

THAM (5 min) induces intracellular alkalization in human HepG2 hepatocytes perfused with acidotic artificial media, with a larger effect in the presence of low extracellular non-bicarbonate buffer compared to high extracellular non-bicarbonate buffer[3].
THAM (110 μL) hydrochloride induces intracellular alkalization in human HepG2 hepatocytes bathed in acidotic human blood[3].
THAM (2-8 mmol/L; 40 min) hydrochloride enhances CO2 gas absorptivity and total inorganic carbon storage capacity of modified BG-11 medium, with the highest TIC absorption observed at 8 mmol·L-1[5].
THAM (2-8 mmol/L; 8 days) hydrochloride improves biomass productivity of Scenedesmus dimorphus in indoor shake flask culture, while also increasing polyunsaturated fatty acid content and showing no biodegradation over 8 days[5].
THAM (6 mmol/L; 8 days) hydrochloride increases biomass areal productivity of Scenedesmus dimorphus by 32-33% and CO2 utilisation efficiency by 6-12% in outdoor 2 m2 raceway pond culture, while also increasing polyunsaturated fatty acid content[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

THAM hydrochloride (≥99%, for cell culture) Related Antibodies

Cell Proliferation Assay[5]

Cell Line: Scenedesmus dimorphus
Concentration: 2, 4, 6, 8 mmol/L
Incubation Time: 8 days
Result: Elevated algal biomass dose-dependently with optimal efficacy at 6 mmol/L.
Achieved 1.40 g/L biomass and 161.59 mg/(L·d), 66% above control values.
Boosted polyunsaturated fatty acids yet lowered C16-C18 fractions with rising doses; total lipid remained ~20%.

Cell Proliferation Assay[5]

Cell Line: Scenedesmus dimorphus
Concentration: 6 mmol/L
Incubation Time: 8 days (two cultivation batches: 12 Aug-20 Aug and 26 Aug-3 Sep)
Result: Boosted biomass and CO2 utilization in first trial, with productivity +33% and CO2 efficiency +12% vs control.
Elevated related indexes moderately in second trial, with 32% higher productivity and 6% improved CO2 efficiency.
Upregulated polyunsaturated fatty acid in two separate culture batches consistently.
Retained total lipid at approximately 20% across both batches.
In Vivo

THAM (weight in kg × 9.2 mEq; i.v.; 1 hour or 3 hours) hydrochloride reduces pulmonary vascular resistance and maintains lower pulmonary arterial pressure in lung-injured piglets with hypercapnia, even after pH rebounds to control levels, while increasing base excess and inducing a post-infusion rebound in PaCO2[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Piglets (23.0-30.5 kg body weight; acute respiratory distress syndrome induced by repeated lung lavages with 30 mL/kg 37-39 °C normal saline, eight total lavages)[1]
Dosage: weight in kg × 9.2 mEq (1-hour infusion group); weight in kg × 9.2 mEq (3-hour infusion group)
Administration: i.v.; 1 hour (1T group); 3 hours (3T group)
Result: Elevated arterial pH to 7.34 (1T) and 7.35 (3T) at 60 min.
Dropped PaCO₂ to 10.2 kPa (1T) and 10.6 kPa (3T) at 60 min.
Raised BE to 13.8 mEq/L (1T) and 16.0 mEq/L (3T) at 60 min, with sustained high BE at 6 h.
Triggered 6 h PaCO₂ rebound alongside synchronous pH decline in both cohorts.
Reduced PVR for both groups relative to control at 6 h.
Cut 3T group MPAP versus baseline control at 6 h.
Boosted 3T group CI above control levels at 6 h.
Decreased serum Na and elevated serum K throughout infusion in two groups.
Upregulated lung IL-6 in 3T group, with unchanged TNF-α and IL-6 across all groups.
Preserved equivalent lung mechanical parameters versus control groups.
Molecular Weight

157.60

Formula

C4H12ClNO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCC(CO)(N)CO.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (634.52 mM)

DMSO : 35 mg/mL (222.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.3452 mL 31.7259 mL 63.4518 mL
5 mM 1.2690 mL 6.3452 mL 12.6904 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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=
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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 6.3452 mL 31.7259 mL 63.4518 mL 158.6294 mL
5 mM 1.2690 mL 6.3452 mL 12.6904 mL 31.7259 mL
10 mM 0.6345 mL 3.1726 mL 6.3452 mL 15.8629 mL
15 mM 0.4230 mL 2.1151 mL 4.2301 mL 10.5753 mL
20 mM 0.3173 mL 1.5863 mL 3.1726 mL 7.9315 mL
25 mM 0.2538 mL 1.2690 mL 2.5381 mL 6.3452 mL
30 mM 0.2115 mL 1.0575 mL 2.1151 mL 5.2876 mL
40 mM 0.1586 mL 0.7931 mL 1.5863 mL 3.9657 mL
50 mM 0.1269 mL 0.6345 mL 1.2690 mL 3.1726 mL
60 mM 0.1058 mL 0.5288 mL 1.0575 mL 2.6438 mL
80 mM 0.0793 mL 0.3966 mL 0.7931 mL 1.9829 mL
100 mM 0.0635 mL 0.3173 mL 0.6345 mL 1.5863 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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THAM hydrochloride (≥99%, for cell culture) Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

THAM hydrochloride (≥99%, for cell culture)1185-53-1Tris HCl (≥99%, for cell culture) Tris hydrochloride (≥99%, for cell culture)Biochemical Assay Reagentslimbal epithelial cellshuman amniotic membranecardiac failurediabetic ketoacidosisScenedesmus dimorphusrespiratory failurehuman HepG2 hepatocytesacute respiratory distress syndromerenal acidosisacidaemiaInhibitorinhibitorinhibit

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