1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Guanidine hydrochloride

Guanidine hydrochloride (Synonyms: Guanidinium chloride; Aminoformamidine hydrochloride)

Cat. No.: HY-B0178A Purity: ≥98.0%
Handling Instructions

Guanidine hydrochloride (Guanidinium chloride) a strong chaotrope, is also a strong denaturant of proteins.

For research use only. We do not sell to patients.

Guanidine hydrochloride Chemical Structure

Guanidine hydrochloride Chemical Structure

CAS No. : 50-01-1

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE Guanidine hydrochloride

    Guanidine hydrochloride purchased from MCE. Usage Cited in: Virol J. 2018 Jan 3;15(1):1.

    Immunofluorescence analysis is performed on RD and A549 cells infected with EV71 at an MOI of 10 (in RD) and 50 (in A549) in the presence or absence of 2.5 mM GnHCl.
    • Biological Activity

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    • Customer Review

    Description

    Guanidine hydrochloride (Guanidinium chloride) a strong chaotrope, is also a strong denaturant of proteins[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Guanidine hydrochloride at low concentrations refolds acid-unfolded apomyoglobin and cytochrome c, stabilizing the molten globule state, i.e. a compact denatured state with a significant amount of secondary structure, but substantially disordered tertiary structure. Guanidine hydrochloride (> 1 M) causes co-operative unfolding of the molten globule state[1].
    A considerable degree of ordered structure remains due to the presence of disulfide bonds, which Guanidine hydrochloride does not affect[2].
    Guanidine hydrochloride at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine hydrochloride in growth media cures [PSI+] and other prions of yeast. 5 mM Guanidine hydrochloride significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine hydrochloride also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    A significant reduction in the death rate of infant mice infected with ten LD50) of coxsackievirus A16 is observed when they are treated 58 h after infection with two injections of Guanidine hydrochloride at 145 mg/kg per injection[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    95.53

    Formula

    CH₆ClN₃

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (1046.79 mM)

    H2O : ≥ 50 mg/mL (523.40 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 10.4679 mL 52.3396 mL 104.6792 mL
    5 mM 2.0936 mL 10.4679 mL 20.9358 mL
    10 mM 1.0468 mL 5.2340 mL 10.4679 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (1046.79 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: ≥98.0%

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    Product Name:
    Guanidine hydrochloride
    Cat. No.:
    HY-B0178A
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