1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Guanidine thiocyanate

Guanidine thiocyanate is a strong protein denaturant and potent inhibitor of nucleases. Guanidinium thiocyanate is a nucleic acid protector in the extraction of DNA and RNA from cells. Guanidine thiocyanate is a common component of buffers used for nucleic acid extraction.

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Guanidine thiocyanate Chemical Structure

Guanidine thiocyanate Chemical Structure

CAS No. : 593-84-0

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Guanidine thiocyanate:

Top Publications Citing Use of Products
IF

    Guanidine thiocyanate purchased from MedChemExpress. Usage Cited in: Virol J. 2018 Jan 3;15(1):1.  [Abstract]

    Immunofluorescence analysis is performed on RD and A549 cells infected with EV71 at an MOI of 10 (in RD) and 50 (in A549) in the presence or absence of 2.5 mM GnHCl.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Guanidine thiocyanate is a strong protein denaturant and potent inhibitor of nucleases. Guanidinium thiocyanate is a nucleic acid protector in the extraction of DNA and RNA from cells. Guanidine thiocyanate is a common component of buffers used for nucleic acid extraction[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Guanidine thiocyanate based-nucleic acid extraction buffers can effectively inactivate high-consequence enveloped viruses, such as Ebola virus, Marburg virus, and Rift Valley Fever virus, as well as West Nile virus, influenza, Venezuelan equine encephalitis virus, and recently, SARS-CoV-2[1].
    Guanidine thiocyanate-based nucleic acid extraction buffers are an effective means of inactivating poliovirus infectious materials and potentially infectious materials[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    118.16

    Formula

    C2H6N4S

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    SC#N.NC(N)=N

    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (846.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (846.31 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.4631 mL 42.3155 mL 84.6310 mL
    5 mM 1.6926 mL 8.4631 mL 16.9262 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    μL

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.88%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 8.4631 mL 42.3155 mL 84.6310 mL 211.5775 mL
    5 mM 1.6926 mL 8.4631 mL 16.9262 mL 42.3155 mL
    10 mM 0.8463 mL 4.2316 mL 8.4631 mL 21.1578 mL
    15 mM 0.5642 mL 2.8210 mL 5.6421 mL 14.1052 mL
    20 mM 0.4232 mL 2.1158 mL 4.2316 mL 10.5789 mL
    25 mM 0.3385 mL 1.6926 mL 3.3852 mL 8.4631 mL
    30 mM 0.2821 mL 1.4105 mL 2.8210 mL 7.0526 mL
    40 mM 0.2116 mL 1.0579 mL 2.1158 mL 5.2894 mL
    50 mM 0.1693 mL 0.8463 mL 1.6926 mL 4.2316 mL
    60 mM 0.1411 mL 0.7053 mL 1.4105 mL 3.5263 mL
    80 mM 0.1058 mL 0.5289 mL 1.0579 mL 2.6447 mL
    100 mM 0.0846 mL 0.4232 mL 0.8463 mL 2.1158 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Guanidine thiocyanate
    Cat. No.:
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