MES
Based on 4 publication(s) in Google Scholar
MES (2-Morpholinoethanesulphonic acid) is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 4432-31-9
- Formula: C6H13NO4S
- Molecular Weight:195.24
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) MES
More
Biological Activity
MES is a zwitterionic buffer and can provide effective buffering capacity at around pH 6.15. The pKa value of MES changes with temperature, so the change of its pKa value needs to be considered when using it at different temperatures[1].
MES shows good buffering properties in biological experiments, exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), thereby can be used in studies involving metal ions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4432-31-9
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Appearance Solid
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Molecular Weight 195.24
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Formula C6H13NO4S
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Color White to off-white
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SMILES
O=S(CCN1CCOCC1)(O)=O
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Synonyms
2-Morpholinoethanesulphonic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (4)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
A novel spherical GelMA-HAMA hydrogel encapsulating APET×2 polypeptide and CFIm25-targeting sgRNA for immune microenvironment modulation and nucleus pulposus regeneration in intervertebral discs. [Abstract]2024 Sep 12;22(1):556. PMID: 39267105 -
Phytomedicine
Da-Bu-Yin-Wan rescues cognitive deficits in aging and Alzheimer's disease models by Wnt/β-catenin-dependent restoration of lysosomal acidification. [Abstract]2026 Apr:153:157916. PMID: 41662806 -
J Colloid Interface Sci
Enhanced photostability and targeting ability of hollow mesoporous manganese-based nanocarriers for NIR-II fluorescence image-guided surgery and photothermal therapy. [Abstract]2025 Nov 15:698:138094. PMID: 40494115 -
Cell Biol Toxicol
IGFBP3 enhances adipose-derived stem cell function in soft tissue injury repair via ITGB1 and ERK pathway activation. [Abstract]2025 May 15;41(1):85. PMID: 40369223
Solvent & Solubility
H2O : 125 mg/mL (640.24 mM; Need ultrasonic)
DMSO : 50 mg/mL (256.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (256.10 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. [Content Brief]
[2]. Tomoko Kagenishi, et al. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. Front Plant Sci. 2016 Feb 18;7:79. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 5.1219 mL | 25.6095 mL | 51.2190 mL | 128.0475 mL |
| 5 mM | 1.0244 mL | 5.1219 mL | 10.2438 mL | 25.6095 mL | |
| 10 mM | 0.5122 mL | 2.5610 mL | 5.1219 mL | 12.8048 mL | |
| 15 mM | 0.3415 mL | 1.7073 mL | 3.4146 mL | 8.5365 mL | |
| 20 mM | 0.2561 mL | 1.2805 mL | 2.5610 mL | 6.4024 mL | |
| 25 mM | 0.2049 mL | 1.0244 mL | 2.0488 mL | 5.1219 mL | |
| 30 mM | 0.1707 mL | 0.8537 mL | 1.7073 mL | 4.2683 mL | |
| 40 mM | 0.1280 mL | 0.6402 mL | 1.2805 mL | 3.2012 mL | |
| 50 mM | 0.1024 mL | 0.5122 mL | 1.0244 mL | 2.5610 mL | |
| 60 mM | 0.0854 mL | 0.4268 mL | 0.8537 mL | 2.1341 mL | |
| 80 mM | 0.0640 mL | 0.3201 mL | 0.6402 mL | 1.6006 mL | |
| 100 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2805 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
MES Related Classifications
HY-D0858 Related Classifications
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