MES monohydrate
Based on 4 publication(s) in Google Scholar
MES (2-Morpholinoethanesulphonic acid) monohydrate is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES monohydrate is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 145224-94-8
- Formula: C6H15NO5S
- Molecular Weight:213.25
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MES monohydrate
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Biological Activity
MES monohydrate is a zwitterionic buffer and can provide effective buffering capacity at around pH 6.15. The pKa value of MES monohydrate changes with temperature, so the change of its pKa value needs to be considered when using it at different temperatures[1].
MES monohydrate shows good buffering properties in biological experiments, exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), thereby can be used in studies involving metal ions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 145224-94-8
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Appearance Solid
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Molecular Weight 213.25
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Formula C6H15NO5S
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Color White to off-white
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SMILES
O=S(CCN1CCOCC1)(O)=O.[H]O[H]
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Synonyms
2-Morpholinoethanesulphonic acid monohydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (4)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
A novel spherical GelMA-HAMA hydrogel encapsulating APET×2 polypeptide and CFIm25-targeting sgRNA for immune microenvironment modulation and nucleus pulposus regeneration in intervertebral discs. [Abstract]2024 Sep 12;22(1):556. PMID: 39267105 -
Phytomedicine
Da-Bu-Yin-Wan rescues cognitive deficits in aging and Alzheimer's disease models by Wnt/β-catenin-dependent restoration of lysosomal acidification. [Abstract]2026 Apr:153:157916. PMID: 41662806 -
J Colloid Interface Sci
Enhanced photostability and targeting ability of hollow mesoporous manganese-based nanocarriers for NIR-II fluorescence image-guided surgery and photothermal therapy. [Abstract]2025 Nov 15:698:138094. PMID: 40494115 -
Cell Biol Toxicol
IGFBP3 enhances adipose-derived stem cell function in soft tissue injury repair via ITGB1 and ERK pathway activation. [Abstract]2025 May 15;41(1):85. PMID: 40369223
Solvent & Solubility
H2O : ≥ 100 mg/mL (468.93 mM)
DMSO : 8.75 mg/mL (41.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.43 mg/mL (6.71 mM); Clear solution
This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.43 mg/mL (6.71 mM); Clear solution
This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. [Content Brief]
[2]. Tomoko Kagenishi, et al. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. Front Plant Sci. 2016 Feb 18;7:79. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.6893 mL | 23.4467 mL | 46.8933 mL | 117.2333 mL |
| 5 mM | 0.9379 mL | 4.6893 mL | 9.3787 mL | 23.4467 mL | |
| 10 mM | 0.4689 mL | 2.3447 mL | 4.6893 mL | 11.7233 mL | |
| 15 mM | 0.3126 mL | 1.5631 mL | 3.1262 mL | 7.8156 mL | |
| 20 mM | 0.2345 mL | 1.1723 mL | 2.3447 mL | 5.8617 mL | |
| 25 mM | 0.1876 mL | 0.9379 mL | 1.8757 mL | 4.6893 mL | |
| 30 mM | 0.1563 mL | 0.7816 mL | 1.5631 mL | 3.9078 mL | |
| 40 mM | 0.1172 mL | 0.5862 mL | 1.1723 mL | 2.9308 mL | |
| H2O | 50 mM | 0.0938 mL | 0.4689 mL | 0.9379 mL | 2.3447 mL |
| 60 mM | 0.0782 mL | 0.3908 mL | 0.7816 mL | 1.9539 mL | |
| 80 mM | 0.0586 mL | 0.2931 mL | 0.5862 mL | 1.4654 mL | |
| 100 mM | 0.0469 mL | 0.2345 mL | 0.4689 mL | 1.1723 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
MES monohydrate Related Classifications
HY-D0858A Related Classifications
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