MES sodium salt (Synonyms: 2-Morpholinoethanesulphonic acid sodium salt)

Cat. No.: HY-D0858B Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MES (2-Morpholinoethanesulphonic acid) sodium salt is a buffering agent in biology and biochemistry. MES sodium salt is one of the Good's buffers, the buffer capacity ranging pH 5.5-7.0. MES sodium salt is broadly used to regulate pH value for plants culture medium, reagent solution, and physiological experiments.

For research use only. We do not sell to patients.

MES sodium salt Chemical Structure

CAS No. : 71119-23-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
10 g	USD 30	In-stock	Estimated Time of Arrival: December 31
50 g	USD 90	In-stock	Estimated Time of Arrival: December 31
> 100 g		Get quote

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Other Forms of MES sodium salt:

MES In-stock
MES monohydrate In-stock
MES hydrate Get quote
MES hemisodium Get quote
MES potassium Get quote
MES-d₁₃ Get quote

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

217.22

Formula

C₆H₁₂NNaO₄S

CAS No.

71119-23-8

Appearance

Solid

Color

White to off-white

SMILES

O=S(CCN1CCOCC1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (115.09 mM; Need ultrasonic)

DMSO : 25 mg/mL (115.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6036 mL	23.0181 mL	46.0363 mL
5 mM	0.9207 mL	4.6036 mL	9.2073 mL
10 mM	0.4604 mL	2.3018 mL	4.6036 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (248 KB) HNMR (235 KB) MS (80 KB)

Handling Instructions (2659 KB)

References

[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. [Content Brief]

[2]. Tomoko Kagenishi, et al. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. Front Plant Sci. 2016 Feb 18;7:79. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.6036 mL	23.0181 mL	46.0363 mL	115.0907 mL
	5 mM	0.9207 mL	4.6036 mL	9.2073 mL	23.0181 mL
	10 mM	0.4604 mL	2.3018 mL	4.6036 mL	11.5091 mL
	15 mM	0.3069 mL	1.5345 mL	3.0691 mL	7.6727 mL
	20 mM	0.2302 mL	1.1509 mL	2.3018 mL	5.7545 mL
	25 mM	0.1841 mL	0.9207 mL	1.8415 mL	4.6036 mL
	30 mM	0.1535 mL	0.7673 mL	1.5345 mL	3.8364 mL
	40 mM	0.1151 mL	0.5755 mL	1.1509 mL	2.8773 mL
	50 mM	0.0921 mL	0.4604 mL	0.9207 mL	2.3018 mL
	60 mM	0.0767 mL	0.3836 mL	0.7673 mL	1.9182 mL
	80 mM	0.0575 mL	0.2877 mL	0.5755 mL	1.4386 mL
	100 mM	0.0460 mL	0.2302 mL	0.4604 mL	1.1509 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

MES sodium salt Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MES71119-23-82-Morpholinoethanesulphonic acidBiochemical Assay ReagentsGood's buffersplants culture mediumreagent solutionphysiological experimentsInhibitorinhibitorinhibit

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