2. GPCR/G Protein
  3. Formyl Peptide Receptor (FPR)
  4. BOC-FlFlF

BOC-FlFlF  (Synonyms: BOC2; Boc-Phe-dLeu-Phe-dLeu-Phe)

製品番号: HY-P2355 純度: 98.31%
COA 取扱説明書 Technical Support

BOC-FlFlF (Boc-Phe-dLeu-Phe-dLeu-Phe) is a selective FPR1 antagonist. Boc-FlFlF has an apparent dissociation constant (KD) of 230 nM as determined by the intracellular calcium mobilization assay. Boc-FlFlF can be used for the study of inflammation.

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BOC-FlFlF

BOC-FlFlF 構造式

CAS 番号 : 66556-73-8

容量 価格（税別） 在庫状況 数量
5 mg $150 在庫あり
10 mg $240 在庫あり
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100 mg   お問い合わせ  

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Other Forms of BOC-FlFlF:

BOC-FlFlF 関連抗体

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製品説明

BOC-FlFlF (Boc-Phe-dLeu-Phe-dLeu-Phe) is a selective FPR1 antagonist. Boc-FlFlF has an apparent dissociation constant (KD) of 230 nM as determined by the intracellular calcium mobilization assay. Boc-FlFlF can be used for the study of inflammation[1][2].

IC50 & Target[1]

FPR1

 

Cellular Effect
Cell Line Type Value Description References
HL-60 IC50
2000 1
Compound: 1
Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
[PMID: 25035262]
HL-60 IC50
2 3
Compound: 1
Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
[PMID: 25035262]
HL-60 IC50
2000 1
Compound: 1
Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
[PMID: 25035262]
体外実験

Boc-FlFlF (1 μM) exerts a selective antagonistic effect on FPR1 and does not inhibit the increase in intracellular calcium concentration induced by the FPR2-selective agonist MMK-1[1].
Boc-FlFlF (1 μM) has an apparent dissociation constant (KD) of 230 nM as determined by the intracellular calcium mobilization assay[1].
Boc-FlFlF (0.0001-100 µM, 5 min pre-treatment) inhibits fMLF (FPR agonist)-induced superoxide production in neutrophils in a concentration-dependent manner with an EC50of 0.25 µM [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BOC-FlFlF 関連抗体
分子量

785.97

分子式

C44H59N5O8
CAS 番号
Appearance

Solid

Color

White to off-white

Sequence

Boc-Phe-dLeu-Phe-dLeu-Phe
Sequence Shortening

Boc-F-dLeu-F-dLeu-F
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

polypeptide, sealed storage, away from moisture and light, under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light, under nitrogen)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (127.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2723 mL 6.3616 mL 12.7231 mL
5 mM 0.2545 mL 1.2723 mL 2.5446 mL
10 mM 0.1272 mL 0.6362 mL 1.2723 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (3.18 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2723 mL 6.3616 mL 12.7231 mL 31.8078 mL
5 mM 0.2545 mL 1.2723 mL 2.5446 mL 6.3616 mL
10 mM 0.1272 mL 0.6362 mL 1.2723 mL 3.1808 mL
15 mM 0.0848 mL 0.4241 mL 0.8482 mL 2.1205 mL
20 mM 0.0636 mL 0.3181 mL 0.6362 mL 1.5904 mL
25 mM 0.0509 mL 0.2545 mL 0.5089 mL 1.2723 mL
30 mM 0.0424 mL 0.2121 mL 0.4241 mL 1.0603 mL
40 mM 0.0318 mL 0.1590 mL 0.3181 mL 0.7952 mL
50 mM 0.0254 mL 0.1272 mL 0.2545 mL 0.6362 mL
60 mM 0.0212 mL 0.1060 mL 0.2121 mL 0.5301 mL
80 mM 0.0159 mL 0.0795 mL 0.1590 mL 0.3976 mL
100 mM 0.0127 mL 0.0636 mL 0.1272 mL 0.3181 mL
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BOC-FlFlF Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BOC-FlFlF66556-73-8BOC2 Boc-Phe-dLeu-Phe-dLeu-PheBOC 2BOC-2Formyl Peptide Receptor (FPR)formylpeptidereceptorFPRBoc-MLFBoc-FLFLFInhibitorinhibitorinhibit

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製品名:
BOC-FlFlF
製品番号:
HY-P2355
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