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  3. BODIPY FL L-Cystine

BODIPY FL L-Cystine is a thiol-reactive, green-fluorescent dye. BODIPY FL L-Cystine can be the labeling of membrane proteins, proteins with hydrophobic binding sites, or hydrophobic ligands. (λex=504 nm, λem=511 nm).

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BODIPY FL L-Cystine

BODIPY FL L-Cystine Chemische Struktur

CAS. Nr. : 1180490-57-6

Größe Preis Verfügbarkeit Menge
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
Auf Lager
Solution
10 mM * 1 mL in DMSO Auf Lager
Solid
5 mg Auf Lager
10 mg Auf Lager
25 mg Auf Lager
50 mg Auf Lager
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500 mg   Angebot einholen  

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1 Publications Citing Use of MCE BODIPY FL L-Cystine

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Beschreibung

BODIPY FL L-Cystine is a thiol-reactive, green-fluorescent dye. BODIPY FL L-Cystine can be the labeling of membrane proteins, proteins with hydrophobic binding sites, or hydrophobic ligands. (λex=504 nm, λem=511 nm)[1][2].

In Vitro

BODIPY FL L-Cystine is composed of two BODIPY molecules and a cysteine residue for detection of reducing environments or the degradation process of disulfide-containing molecules.
BODIPY FL L-Cystine can be labeled to disulfide-containing polymers (such as PEI or pLL). As the disulfide bonds on the polymer be cut by the reducing environment, the self-quenching effect will be reduced, and the fluorescence intensity is increased[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

788.45

Formel

C34H38B2F4N6O6S2

CAS. Nr.
Appearance

Solid

Color

Orange to red

Emission (Em)

510

Excitation (Ex)

502

SMILES

[F-][B+3]([F-])([N-]12)[N]3=C(C)C=C(C)C3=CC1=CC=C2CCC(N[C@@H](CSSC[C@@H](C(O)=O)NC(CCC4=CC=C5[N-]4[B+3]([F-])([F-])[N]6=C(C)C=C(C)C6=C5)=O)C(O)=O)=O

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 50 mg/mL (63.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2683 mL 6.3416 mL 12.6831 mL
5 mM 0.2537 mL 1.2683 mL 2.5366 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation

Purity: 99.14%

Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2683 mL 6.3416 mL 12.6831 mL 31.7078 mL
5 mM 0.2537 mL 1.2683 mL 2.5366 mL 6.3416 mL
10 mM 0.1268 mL 0.6342 mL 1.2683 mL 3.1708 mL
15 mM 0.0846 mL 0.4228 mL 0.8455 mL 2.1139 mL
20 mM 0.0634 mL 0.3171 mL 0.6342 mL 1.5854 mL
25 mM 0.0507 mL 0.2537 mL 0.5073 mL 1.2683 mL
30 mM 0.0423 mL 0.2114 mL 0.4228 mL 1.0569 mL
40 mM 0.0317 mL 0.1585 mL 0.3171 mL 0.7927 mL
50 mM 0.0254 mL 0.1268 mL 0.2537 mL 0.6342 mL
60 mM 0.0211 mL 0.1057 mL 0.2114 mL 0.5285 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BODIPY FL L-Cystine
Art. -Nr.:
HY-D1605
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