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  4. Calcein (mixture of isomers)

Calcein (mixture of isomers)  (Synonyms: カルセイン (異性体混合物))

製品番号: HY-D1249
COA 取扱説明書 Technical Support

Calcein (mixture of isomers), Calcein (HY-D0040) Derivative, is a fluorescent dye (Ex/Em = 495/515 nm). Calcein (mixture of isomers) can be used for the researches for live cell staining, calcium ion detection, bone fluorescence labeling and membrane permeability leakage detection.

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Calcein (mixture of isomers)

Calcein (mixture of isomers) 構造式

CAS 番号 : 154071-48-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
10 mg $30 在庫あり
25 mg $50 在庫あり
50 mg $80 在庫あり
100 mg $120 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Calcein (mixture of isomers) 関連抗体

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製品説明

Calcein (mixture of isomers), Calcein (HY-D0040) Derivative, is a fluorescent dye (Ex/Em = 495/515 nm). Calcein (mixture of isomers) can be used for the researches for live cell staining, calcium ion detection, bone fluorescence labeling and membrane permeability leakage detection.

体外実験

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Take live cell staining as an example:
(1) Preparation of stock solution: Prepare a 10 mM stock solution with anhydrous DMSO and store it at -20℃.
(2) Preparation of working solution: Dilute with serum-free buffer (or PBS) to a final concentration of 0.1-10 μM, and prepare it freshly before use.
(3) Staining: Aspirate the old medium, add 1-10 μM Calcein working solution, and incubate at 37℃ in the dark for 15-60 min.
(4) Fluorescence detection: Wash 3 times with preheated PBS, replace with fresh medium, and then perform imaging detection.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Calcein (mixture of isomers) 関連抗体
分子量

622.53

分子式

C30H26N2O13
Emission (Em)

512
Excitation (Ex)

488
CAS 番号
Appearance

Solid

Color

Yellow to orange

SMILES

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C=C4)OC5=C3C=CC(O)=C5.CCN(CC(O)=O)CC(O)=O.CCN(CC(O)=O)CC(O)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (160.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1M NaOH : 10 mg/mL (16.06 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6063 mL 8.0317 mL 16.0635 mL
5 mM 0.3213 mL 1.6063 mL 3.2127 mL
10 mM 0.1606 mL 0.8032 mL 1.6063 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション

COA (253 KB)

取扱説明書 (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
1M NaOH / DMSO 1 mM 1.6063 mL 8.0317 mL 16.0635 mL 40.1587 mL
5 mM 0.3213 mL 1.6063 mL 3.2127 mL 8.0317 mL
10 mM 0.1606 mL 0.8032 mL 1.6063 mL 4.0159 mL
15 mM 0.1071 mL 0.5354 mL 1.0709 mL 2.6772 mL
DMSO 20 mM 0.0803 mL 0.4016 mL 0.8032 mL 2.0079 mL
25 mM 0.0643 mL 0.3213 mL 0.6425 mL 1.6063 mL
30 mM 0.0535 mL 0.2677 mL 0.5354 mL 1.3386 mL
40 mM 0.0402 mL 0.2008 mL 0.4016 mL 1.0040 mL
50 mM 0.0321 mL 0.1606 mL 0.3213 mL 0.8032 mL
60 mM 0.0268 mL 0.1339 mL 0.2677 mL 0.6693 mL
80 mM 0.0201 mL 0.1004 mL 0.2008 mL 0.5020 mL
100 mM 0.0161 mL 0.0803 mL 0.1606 mL 0.4016 mL
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Calcein (mixture of isomers) Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Calcein (mixture of isomers)154071-48-4Fluorescent Dyebone metabolismcalcium fluorescence detectionInhibitorinhibitorinhibit

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製品名:
Calcein (mixture of isomers)
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HY-D1249
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