DBCO-PEG4-NHS ester

Name: DBCO-PEG4-NHS ester
Brand: MedChemExpress
SKU: HY-140272
Rating: 5.0 (4 reviews)

製品番号: HY-140272 純度: 99.00%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

DBCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-PEG4-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 1427004-19-0

容量	価格（税別）	在庫状況	数量
>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
5 mg	$40	在庫あり	Estimated Time of Arrival: December 31
10 mg	$64	在庫あり	Estimated Time of Arrival: December 31
25 mg	$110	在庫あり	Estimated Time of Arrival: December 31
50 mg	$165	在庫あり	Estimated Time of Arrival: December 31
100 mg	$280	在庫あり	Estimated Time of Arrival: December 31
250 mg	$485	在庫あり	Estimated Time of Arrival: December 31
500 mg	$720	在庫あり	Estimated Time of Arrival: December 31
1 g	$1188	在庫あり	Estimated Time of Arrival: December 31
5 g		お問い合わせ
10 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 4 publication(s) in Google Scholar

顧客検証

Bio/Physico-chemical Assay

Others

Cell Proliferation/Viability Assay

: DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]; FTIR of DBCO–Chitosan prepared with varying doses of DBCO-PEG4-NHS ester.

: DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]; Representative fluorescence imaging of DBCO-PEG4-NHS ester-modified chitosan membranes incubated with N3–PEG–FITC.

: DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]; Representative stress–strain curves of chitosan membranes with/without DBCO-modified.

: DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]; Scanning electron microscopy (SEM) image of DBCO–Chitosan membranes. Scale bar, 500, and 100 µm (magnification).

: DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]; A live/dead staining assay revealeda decrease in fibroblast viability when the DBCO-PEG4 -NHSester concentration reached 2.5 µm.Cellular toxicity of chitosan materials before and after DBCO modification. a) Live/dead staining of chitosan membranes with or without DBCO modification with Calcein-AM (1:1000) and PI (1:800) staining at 48 and 72 h. Scale bar, 50 μm. b) The cell viability of L929 cells in each group at 48 h (n = 3). Data are expressed as mean ± SD. ****p < 0.001, and ns represent p﹥0.05. Data were analyzed by Two-tailed Student’s t-tests for pairwise group comparisons.

PROTAC Linkers アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

PEG Linkers Alkyl-Chain Linkers Alkyl/ether Linkers

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

DBCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs^[1]. DBCO-PEG4-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC₅₀ & Target

PEGs

体外実験

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

649.69

分子式

C₃₄H₃₉N₃O₁₀

CAS 番号

1427004-19-0

Appearance

Solid-Liquid Mixture

Color

Light yellow to brown

SMILES

O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCNC(CCC(N2C3=CC=CC=C3C#CC4=CC=CC=C4C2)=O)=O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 70 mg/mL (107.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5392 mL	7.6960 mL	15.3920 mL
5 mM	0.3078 mL	1.5392 mL	3.0784 mL
10 mM	0.1539 mL	0.7696 mL	1.5392 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (3.20 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.00%

Select Batch:

データシート (270 KB) SDS (393 KB)

COA (243 KB) HNMR (232 KB) RP-HPLC (248 KB) MS (156 KB)

取扱説明書 (2659 KB)

参考文献

[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5392 mL	7.6960 mL	15.3920 mL	38.4799 mL
	5 mM	0.3078 mL	1.5392 mL	3.0784 mL	7.6960 mL
	10 mM	0.1539 mL	0.7696 mL	1.5392 mL	3.8480 mL
	15 mM	0.1026 mL	0.5131 mL	1.0261 mL	2.5653 mL
	20 mM	0.0770 mL	0.3848 mL	0.7696 mL	1.9240 mL
	25 mM	0.0616 mL	0.3078 mL	0.6157 mL	1.5392 mL
	30 mM	0.0513 mL	0.2565 mL	0.5131 mL	1.2827 mL
	40 mM	0.0385 mL	0.1924 mL	0.3848 mL	0.9620 mL
	50 mM	0.0308 mL	0.1539 mL	0.3078 mL	0.7696 mL
	60 mM	0.0257 mL	0.1283 mL	0.2565 mL	0.6413 mL
	80 mM	0.0192 mL	0.0962 mL	0.1924 mL	0.4810 mL
	100 mM	0.0154 mL	0.0770 mL	0.1539 mL	0.3848 mL