DPPH (Synonyms: 2,2-Diphenyl-1-picrylhydrazyl)

Name: DPPH
Brand: MedChemExpress
SKU: HY-112053
Rating: 5.0 (46 reviews)

Cat. No.: HY-112053 Purity: 98.77%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical that can be used to measure the radical scavenging activity of antioxidants. The odd electron of nitrogen atom in DPPH is reduced by receiving a hydrogen atom from antioxidants to the corresponding hydrazine. DPPH method may be utilized in aqueous and nonpolar organic solvents and can be used to examine both hydrophilic and lipophilic antioxidants.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 1898-66-4

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 46 publication(s) in Google Scholar

Other Forms of DPPH:

DPPH (Standard) In-stock

46 Publications Citing Use of MCE DPPH

Bio/Physico-chemical Assay

: DPPH purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Jun 7:52:200-212. [Abstract]; DPPH (100 μM; 30 min). DPPH scavenging capabilities of PCL, PCL-PDA, PCL-PDA-GM, and PCL-PDA-GM + NIR scaffolds.

: DPPH purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Feb 20:31:101598. [Abstract]; DPPH (300 μM; 30 min). Scavenging rates of DPPH radicals scavenged by TA-Fe NPs at different concentrations.

: DPPH purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 24;22(1):653. [Abstract]; DPPH (20 μM; 30 min). Quantitative analysis of the scavenging rate of DPPH radicals by Cur, CurNPs, and HA-CurNPs.

: DPPH purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Feb;10(6):e2204006. [Abstract]; DPPH (100 μM; 30 min). DPPH assay to test the ability of 5‐HT (10 µM) as radical trapping agent, fer‐1 (10 µM), Lipro‐1 (10 µM), and Vitamin E (10 µM) as positive controls.

: DPPH purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2021 Oct 6;13(39):46391-46405. [Abstract]; DPPH (32.5 μM; 30 min). Antioxidant activity was measured by DPPH assay.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

DPPH shows a strong absorption band at 517 nm due to its odd electron and solution appears a deep violet colour, the absorption vanishes as the electron pairs off. The resulting decolorization is stoichiometric with respect to the number of electrons taken up. The alcoholic solutions of 0.5 mM are densely colored and at this concentration, the Lambert-Beer law is obeyed over the useful range of absorption^[1].
DPPH assay is a rapid, simple, inexpensive and widely used method to measure the ability of compounds to act as free radical scavengers or hydrogen donors, and to evaluate antioxidant activity of foods. It can also be used to quantify antioxidants in complex biological systems, for solid or liquid samples. This method is easy and applies to measure the overall antioxidant capacity and the free radical scavenging activity of fruit and vegetable juice. This assay has been successfully utilized for investigating antioxidant properties of wheat grain and bran, vegetables, conjugated linoleic acids, herbs, edible seed oils, and flours in several different solvent systems including ethanol, aqueous acetone, methanol, aqueous alcohol and benzene. It is a convenient method for the antioxidant assay of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds, for olive oil, fruits, juices and wines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

394.32

화학식

C₁₈H₁₂N₅O₆

CAS No.

1898-66-4

Appearance

Solid

Color

Brown to black

SMILES

O=[N+](C1=C([N]N(C2=CC=CC=C2)C3=CC=CC=C3)C([N+]([O-])=O)=CC([N+]([O-])=O)=C1)[O-]

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 25 mg/mL (63.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5360 mL	12.6801 mL	25.3601 mL
5 mM	0.5072 mL	2.5360 mL	5.0720 mL
10 mM	0.2536 mL	1.2680 mL	2.5360 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 98.81%

Select Batch:

Data Sheet (278 KB) SDS (419 KB)

COA (254 KB) RP-HPLC (206 KB)

Handling Instructions (2659 KB)

References

[1]. Kedare SB, et al. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011 Aug;48(4):412-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5360 mL	12.6801 mL	25.3601 mL	63.4003 mL
	5 mM	0.5072 mL	2.5360 mL	5.0720 mL	12.6801 mL
	10 mM	0.2536 mL	1.2680 mL	2.5360 mL	6.3400 mL
	15 mM	0.1691 mL	0.8453 mL	1.6907 mL	4.2267 mL
	20 mM	0.1268 mL	0.6340 mL	1.2680 mL	3.1700 mL
	25 mM	0.1014 mL	0.5072 mL	1.0144 mL	2.5360 mL
	30 mM	0.0845 mL	0.4227 mL	0.8453 mL	2.1133 mL
	40 mM	0.0634 mL	0.3170 mL	0.6340 mL	1.5850 mL
	50 mM	0.0507 mL	0.2536 mL	0.5072 mL	1.2680 mL
	60 mM	0.0423 mL	0.2113 mL	0.4227 mL	1.0567 mL

DPPH Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DPPH1898-66-42,2-Diphenyl-1-picrylhydrazylReactive Oxygen Species (ROS)Inhibitorinhibitorinhibit

최근 본 상품:

상품명

Requested Quantity *

고객명 *

호칭

메일주소 *

전화번호 *

Department

회사명 *

City

도

Country or Region *

비고

상품명

Lot #

고객명 *

메일주소 *

전화번호

Department *

회사명 *

Country or Region *

비고

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

DPPH (Synonyms: 2,2-Diphenyl-1-picrylhydrazyl)

고객리뷰

Other Forms of DPPH:

DPPH Related Antibodies

46 Publications Citing Use of MCE DPPH

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

DPPH Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

DPPH Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report