DTME (Synonyms: Dithio-bis-maleimidoethane)

製品番号: HY-W106411 純度: 99.03%: Data Sheet SDS COA 取扱説明書
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DTME (dithio-bis-maleimidoethane) is a homobifunctional, maleimide crosslinker specifically designed for conjugation between sulfhydryl groups (-SH). DTME, whose molecular structure consists of two maleimide groups connected by an ethylene disulfide bridge, can specifically react with thiol - containing molecules (such as cysteine residues) to form stable covalent bonds. DTME allows crosslinks that can be cleaved with reducing agents such as DTT (HY-15917). DTME is commonly utilized to explore and characterize protein structure, particularly oligomerization, or protein interactions.

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DTME 構造式

CAS 番号 : 71865-37-7

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 42	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 42	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$39	在庫あり	Estimated Time of Arrival: December 31
10 mg	$60	在庫あり	Estimated Time of Arrival: December 31
25 mg	$116	在庫あり	Estimated Time of Arrival: December 31
50 mg	$185	在庫あり	Estimated Time of Arrival: December 31
100 mg	$290	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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製品説明

分子量

312.36

分子式

C₁₂H₁₂N₂O₄S₂

CAS 番号

71865-37-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(C=C1)N(CCSSCCN2C(C=CC2=O)=O)C1=O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (160.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2014 mL	16.0072 mL	32.0143 mL
5 mM	0.6403 mL	3.2014 mL	6.4029 mL
10 mM	0.3201 mL	1.6007 mL	3.2014 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.03%

Select Batch:

データシート (272 KB) SDS (393 KB)

COA (250 KB) HNMR (257 KB) LCMS (95 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Kovalenko OV, et al. Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking. Biochem J. 2004 Jan 15;377(Pt 2):407-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2014 mL	16.0072 mL	32.0143 mL	80.0359 mL
	5 mM	0.6403 mL	3.2014 mL	6.4029 mL	16.0072 mL
	10 mM	0.3201 mL	1.6007 mL	3.2014 mL	8.0036 mL
	15 mM	0.2134 mL	1.0671 mL	2.1343 mL	5.3357 mL
	20 mM	0.1601 mL	0.8004 mL	1.6007 mL	4.0018 mL
	25 mM	0.1281 mL	0.6403 mL	1.2806 mL	3.2014 mL
	30 mM	0.1067 mL	0.5336 mL	1.0671 mL	2.6679 mL
	40 mM	0.0800 mL	0.4002 mL	0.8004 mL	2.0009 mL
	50 mM	0.0640 mL	0.3201 mL	0.6403 mL	1.6007 mL
	60 mM	0.0534 mL	0.2668 mL	0.5336 mL	1.3339 mL
	80 mM	0.0400 mL	0.2001 mL	0.4002 mL	1.0004 mL
	100 mM	0.0320 mL	0.1601 mL	0.3201 mL	0.8004 mL

DTME Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DTME71865-37-7Dithio-bis-maleimidoethaneBiochemical Assay ReagentsdeoxyribozymeDiels-Alder reactionInhibitorinhibitorinhibit

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