Endoproteinase Lys-C 

Endoproteinase Lys-C is a protease that cleaves proteins on the C-terminal side of lysine residues and is commonly used for protein sequencing^[1].

This product can be used for: (1) protein sequence analysis; (2) synthesis of lysine-related derivatives.

Protocol
This product can be directly used for protein enzyme digestion. The recommended enzyme digestion ratio is 1:50-1:200 (W/W), and the enzyme digestion should be performed at 37 °C overnight.

Notes
( 1) The optimal reaction pH of this product is 9.0-9.5, and the optimal reaction temperature is 30-37 °C. It can still maintain high enzyme activity in the presence of 4 M urea or 0.1% SDS.
( 2) Storage Buffer: 20 mM Tris-HCl, pH=8.0.
( 3) Do not freeze and thaw repeatedly.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

72561-05-8

Solid

White to off-white

[Endoproteinase Lys-C]

3.4.21.50

≥1 AU/mg soild

One activity unit is defined as the amount of enzyme that can catalyzes the substrate to produce 1 μ mol of p-Nitroaniline per minute at 30°C and pH 9.5.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. P A Jekel, et al. Use of endoproteinase Lys-C from Lysobacter enzymogenes in protein sequence analysis. Anal Biochem. 1983 Oct 15;134(2):347-54.   [Content Brief]