2. Cytoskeleton
  3. Exosomes
  4. Endosidin-2

Endosidin-2 (ES2) is a selective inhibitor targeting the conserved exosome subunit EXO70. Endosidin-2 binds to the C-terminal domain of EXO70, inhibiting exocytosis and endosomal recycling, while promoting vacuolar trafficking in plant cells. Endosidin-2 interferes with the EXO70-mediated vesicle-to-plasma membrane anchoring process, leading to abnormal aggregation of auxin transporters (such as PIN2) in the cytoplasm and redirected to vacuolar degradation, while causing abnormal Golgi structure (such as cup-shaped or ring-shaped vesicles cisternae formation). Endosidin-2 can inhibit exocytosis in plant and mammalian cells and is mainly used to study the dynamic regulation of membrane trafficking (such as polar growth, vesicle sorting).

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Endosidin-2

Endosidin-2 Chemical Structure

CAS No. : 1839524-44-5

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Based on 2 publication(s) in Google Scholar

Endosidin-2 Related Antibodies

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Description

Endosidin-2 (ES2) is a selective inhibitor targeting the conserved exosome subunit EXO70. Endosidin-2 binds to the C-terminal domain of EXO70, inhibiting exocytosis and endosomal recycling, while promoting vacuolar trafficking in plant cells. Endosidin-2 interferes with the EXO70-mediated vesicle-to-plasma membrane anchoring process, leading to abnormal aggregation of auxin transporters (such as PIN2) in the cytoplasm and redirected to vacuolar degradation, while causing abnormal Golgi structure (such as cup-shaped or ring-shaped vesicles cisternae formation). Endosidin-2 can inhibit exocytosis in plant and mammalian cells and is mainly used to study the dynamic regulation of membrane trafficking (such as polar growth, vesicle sorting)[1][2].

In Vitro

1. Arabidopsis root epidermal cell PIN2 protein localization experiment: Endosidin-2 (40 μM; 2 h) significantly reduced the PIN2-GFP fluorescence intensity on the plasma membrane, induced PIN2 to accumulate in the ARA7/RabF2b-marked late endosome/pre-vacuolar compartment, inhibited exocytosis and enhanced vacuolar transport.[1].
2. Arabidopsis root epidermal cell multi-protein tracing experiment: Endosidin-2 (50 μM; 1.5-3 h) can cause PIN2, PIN3, and PIN4 to form abnormal aggregates (ES2As) in the cytoplasm, accelerate PIN2 endocytosis and redirect it to the vacuole, without affecting the localization of SYT1 protein, causing the Golgi apparatus to appear cup-shaped or ring-shaped cisternae and other structural abnormalities[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Endosidin-2 Related Antibodies
Essai clinique
Masse moléculaire

414.17

Formule

C15H12FIN2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N/N=C/C1=CC(OC)=C(O)C(I)=C1)C2=CC=CC(F)=C2
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

DMSO : 100 mg/mL (241.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4145 mL 12.0723 mL 24.1447 mL
5 mM 0.4829 mL 2.4145 mL 4.8289 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Pureté et documentation
Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4145 mL 12.0723 mL 24.1447 mL 60.3617 mL
5 mM 0.4829 mL 2.4145 mL 4.8289 mL 12.0723 mL
10 mM 0.2414 mL 1.2072 mL 2.4145 mL 6.0362 mL
15 mM 0.1610 mL 0.8048 mL 1.6096 mL 4.0241 mL
20 mM 0.1207 mL 0.6036 mL 1.2072 mL 3.0181 mL
25 mM 0.0966 mL 0.4829 mL 0.9658 mL 2.4145 mL
30 mM 0.0805 mL 0.4024 mL 0.8048 mL 2.0121 mL
40 mM 0.0604 mL 0.3018 mL 0.6036 mL 1.5090 mL
50 mM 0.0483 mL 0.2414 mL 0.4829 mL 1.2072 mL
60 mM 0.0402 mL 0.2012 mL 0.4024 mL 1.0060 mL
80 mM 0.0302 mL 0.1509 mL 0.3018 mL 0.7545 mL
100 mM 0.0241 mL 0.1207 mL 0.2414 mL 0.6036 mL
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Endosidin-2 Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Endosidin-21839524-44-5ES2Endosidin2Endosidin 2ES 2ES-2ExosomesInhibitorinhibitorinhibit

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