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  3. Fluorescent Dye
  4. FM1-43

FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.

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CAS 番号 : 149838-22-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 471 在庫あり
Solution
10 mM * 1 mL in DMSO USD 471 在庫あり
Solid
1 mg $140 在庫あり
5 mg $350 在庫あり
10 mg $525 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 7 publication(s) in Google Scholar

Other Forms of FM1-43:

FM1-43 関連抗体

Top Publications Citing Use of Products

    FM1-43 purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Jun 4.

    Fluorescence in isolated organs of Corti from WT and Tmem145-/- mice (age: P14-P21) was observed at 180 s of incubation with FM1-43 (10 μM; 170 s). Right, pre-incubation with BAPTA (5 mM, 20 min) abolished FM1-43 uptake.

    FM1-43 purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Jun 4.

    Time course of FM1-43 (10 μM) dye uptake by WT (black) and Tmem145-/- (red) OHCs.

    FM1-43 purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 Aug 19:175639.  [Abstract]

    FM1-43 (1 μM; 37 ℃; 30 min). BMVs were observed by SR-CLSM. S1. The red arrow pointed to the first secreted vesicle, and the white arrow pointed to the second one. Inside the white circle were the already secreted BMVs.

    FM1-43 purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2024 May 31:285:127774.  [Abstract]

    FM1-43 (1 μM; 30 min). OMVs were observed by CLSM, the white arrow pointing OMVs.

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.

    体外実験

    Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
    1. FM Working Fluid Preparation 1.1 Preparation of Storage Solution Prepare a 5 mM stock solution using DMSO.
    1.2 Preparation of Working Solution Prepare the FM 1-43 working solution at a concentration of 5-20 μM using the preheated HBSS solution.
    Note: Please adjust the concentration of the FM 1-43 working solution according to the actual situation, and prepare it as needed.

    2. Cell Staining (Suspension Cells) 2.1 Centrifuge the cells and add PBS for two washes, each for 5 minutes. The cell density is 1×106 cells/mL. 2.2 Add 1 mL of FM working solution and incubate at room temperature for 5 to 30 minutes.
    2.3 400 grams, centrifuge for 3-4 minutes, then discard the supernatant.
    2.4 Add PBS to wash the cells twice, for 5 minutes each time.
    2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
    3. Cytological staining (adherent cells) 3.1 Cultivate adherent cells on a sterile cover glass.
    3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
    3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
    3.4 Remove the dye working solution and wash with the culture medium 2-3 times, for 5 minutes each time. Then observe using a fluorescence microscope or flow cytometer.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    FM1-43 関連抗体
    分子量

    611.54

    分子式

    C30H49Br2N3
    Emission (Em)

    585
    Excitation (Ex)

    480
    CAS 番号
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    CC[N+](CC)(CC)CCC[N+](C=C1)=CC=C1/C=C/C2=CC=C(N(CCCC)CCCC)C=C2.[Br-].[Br-]
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (81.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6352 mL 8.1761 mL 16.3522 mL
    5 mM 0.3270 mL 1.6352 mL 3.2704 mL
    10 mM 0.1635 mL 0.8176 mL 1.6352 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.85%

    COA (247 KB) HNMR (259 KB) RP-HPLC (256 KB) MS (69 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6352 mL 8.1761 mL 16.3522 mL 40.8804 mL
    5 mM 0.3270 mL 1.6352 mL 3.2704 mL 8.1761 mL
    10 mM 0.1635 mL 0.8176 mL 1.6352 mL 4.0880 mL
    15 mM 0.1090 mL 0.5451 mL 1.0901 mL 2.7254 mL
    20 mM 0.0818 mL 0.4088 mL 0.8176 mL 2.0440 mL
    25 mM 0.0654 mL 0.3270 mL 0.6541 mL 1.6352 mL
    30 mM 0.0545 mL 0.2725 mL 0.5451 mL 1.3627 mL
    40 mM 0.0409 mL 0.2044 mL 0.4088 mL 1.0220 mL
    50 mM 0.0327 mL 0.1635 mL 0.3270 mL 0.8176 mL
    60 mM 0.0273 mL 0.1363 mL 0.2725 mL 0.6813 mL
    80 mM 0.0204 mL 0.1022 mL 0.2044 mL 0.5110 mL
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    FM1-43 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    FM1-43149838-22-2Fluorescent Dyestyryl dyeendocytosisexocytosismechanotransducer channelhair cellcochleamechanotransductionion channelaminoglycosidesmyosin VIIAInhibitorinhibitorinhibit

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    製品名:
    FM1-43
    製品番号:
    HY-D1434
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