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  3. Fura-2 pentapotassium

Fura-2 pentapotassium is a cell-impermeable scaled fluorescent dye that can be used for intracellular calcium imaging. Fura-2 pentapotassium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca2+ level.

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Fura-2 pentapotassium

Fura-2 pentapotassium Estructura química

No. CAS : 113694-64-7

Tamaño Precio Stock Cantidad
500 μg En stock
1 mg En stock
5 mg   Obtener un presupuesto  
10 mg   Obtener un presupuesto  

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Descripciòn

Fura-2 pentapotassium is a cell-impermeable scaled fluorescent dye that can be used for intracellular calcium imaging. Fura-2 pentapotassium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca2+ level[1][2].

In Vitro

Fura-2 itself cannot pass through the cell membrane, and various methods (such as microinjection, ATP permeabilization, scrape loading, and pH shock) can be employed to insert Fura-2 into the cytoplasm of cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

831.99

Fòrmula

C29H22K5N3O14

No. CAS
Appearance

Solid

Color

Off-white to light yellow

Emission (Em)

540

Excitation (Ex)

510

SMILES

O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(O[K])=O)CC(O[K])=O)=C(N(CC(O[K])=O)CC(O[K])=O)C=C3O2)O1)O[K]

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvente y solubilidad
In Vitro: 

1M NaOH : 10 mg/mL (12.02 mM; Need ultrasonic and warming)

1M NaOH : 10 mg/mL (12.02 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2019 mL 6.0097 mL 12.0194 mL
5 mM 0.2404 mL 1.2019 mL 2.4039 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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μL

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Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 97.0%

Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
1M NaOH / 1M NaOH 1 mM 1.2019 mL 6.0097 mL 12.0194 mL 30.0484 mL
5 mM 0.2404 mL 1.2019 mL 2.4039 mL 6.0097 mL
10 mM 0.1202 mL 0.6010 mL 1.2019 mL 3.0048 mL
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Inquiry Information

Nombre del producto:
Fura-2 pentapotassium
Cat. No.:
HY-D0110A
Cantidad:
MCE Japan Authorized Agent: