H-Ala-Ala-OH (Synonyms: L-Alanyl-L-alanine; Ala-Ala)

Cat. No.: HY-W010162 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
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H-Ala-Ala-OH is a substrate of human intestinal oligopeptide transporter (PEPT1/SLC15A1), with an IC₅₀ of 0.25 mM and an EC₅₀ of 0.08 mM against human PEPT1. When used as a dipeptide linker in antibody-drug conjugates (ADCs) loaded with glucocorticoid receptor regulator payloads, H-Ala-Ala-OH enables efficient intracellular payload release.

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H-Ala-Ala-OH 화학구조

CAS No. : 1948-31-8

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	해외재고보유	Estimated Time of Arrival: December 31
10 g	견적 받기
50 g	견적 받기

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This product is a controlled substance and not for sale in your territory.

Other Forms of H-Ala-Ala-OH:

H-Ala-Ala-OH (Standard) 견적 받기

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	63 3 Compound: Ala-Ala	TP_TRANSPORTER: inhibition of Gly-Sar uptake (Gly-Sar: 25000 uM) in PEPT1-expressing CHO cells TP_TRANSPORTER: inhibition of Gly-Sar uptake (Gly-Sar: 25000 uM) in PEPT1-expressing CHO cells	[PMID: 8956326]
CHO	IC₅₀	63 3 Compound: Ala-Ala	TP_TRANSPORTER: inhibition of Gly-Sar uptake (Gly-Sar: 25000 uM) in PEPT1-expressing CHO cells TP_TRANSPORTER: inhibition of Gly-Sar uptake (Gly-Sar: 25000 uM) in PEPT1-expressing CHO cells	[PMID: 8956326]
MDCK	EC₅₀	0.08 2 Compound: 3	Activation of human PEPT1 expressed in MDCK cells Activation of human PEPT1 expressed in MDCK cells	[PMID: 16759105]
MDCK	IC₅₀	0.25 2 Compound: 3	Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells	[PMID: 16759105]
MDCK	EC₅₀	0.08 2 Compound: 3	Activation of human PEPT1 expressed in MDCK cells Activation of human PEPT1 expressed in MDCK cells	[PMID: 16759105]
MDCK	IC₅₀	0.25 2 Compound: 3	Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells	[PMID: 16759105]

In Vitro

The ADC containing H₂Ala-Ala-OH (Ala-Ala) as the dipeptide linker potently activates the GRE luciferase reporter in mTNF-overexpressing K562 cells with an EC₅₀ of 0.92 μg mL⁻¹, and shows no activity in wild-type K562 cells, confirming targeted activity^[1].
H-Ala-Ala-OH (0.08-5 mM; 2 min) acts as a highly potent PEPT1 substrate in MDCK-PEPT1 cells, with an EC₅₀ of 0.08 mM and a %GSmax of 125%, resulting in a %GSmax/EC₅₀ ratio of 1700 that classifies it as one of the best PEPT1 substrates tested^[2].
H-Ala-Ala-OH (0.1-9 mM; 30 min preincubation, 10 min uptake period) binds to PEPT1 in MDCK-PEPT1 cells with an IC₅₀ of 0.25 mM, confirming it interacts with the transporter's binding pocket^[2].
H-Ala-Ala-OH exhibits a favorable low-energy conformation for PEPT1 binding and transport in silico, with flexible small side chains enabling ideal interaction with the transporter's binding pocket^[2].
H-Ala-Ala-OH (0.010-100 mM; 30 min) potently inhibits hPEPT1-mediated ³H-Gly-Sar uptake in CHO-PEPT1 cells with an IC₅₀ of 0.063 mM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

분자량

160.17

화학식

C₆H₁₂N₂O₃

CAS No.

1948-31-8

Appearance

Solid

Color

White to off-white

SMILES

C[C@@H](C(O)=O)NC([C@H](C)N)=O

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

용액&용해도

In Vitro:

DMSO : ≥ 200 mg/mL (1248.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.2434 mL	31.2168 mL	62.4337 mL
5 mM	1.2487 mL	6.2434 mL	12.4867 mL
10 mM	0.6243 mL	3.1217 mL	6.2434 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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순도&문서

Purity: 98.0%

Select Batch:

Data Sheet (273 KB) SDS (394 KB)

COA (246 KB) HNMR (207 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Wang L, et al. Impact of dipeptide on ADC physicochemical properties and efficacy identifies Ala-Ala as the optimal dipeptide. RSC Med Chem. 2023;15(1):355-365. Published 2023 Nov 27.

[2]. Vig BS, et al. Human PEPT1 pharmacophore distinguishes between dipeptide transport and binding. J Med Chem. 2006;49(12):3636-3644. [Content Brief]

[3]. Covitz KM, et al. Human dipeptide transporter, hPEPT1, stably transfected into Chinese hamster ovary cells. Pharm Res. 1996;13(11):1631-1634. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.2434 mL	31.2168 mL	62.4337 mL	156.0842 mL
	5 mM	1.2487 mL	6.2434 mL	12.4867 mL	31.2168 mL
	10 mM	0.6243 mL	3.1217 mL	6.2434 mL	15.6084 mL
	15 mM	0.4162 mL	2.0811 mL	4.1622 mL	10.4056 mL
	20 mM	0.3122 mL	1.5608 mL	3.1217 mL	7.8042 mL
	25 mM	0.2497 mL	1.2487 mL	2.4973 mL	6.2434 mL
	30 mM	0.2081 mL	1.0406 mL	2.0811 mL	5.2028 mL
	40 mM	0.1561 mL	0.7804 mL	1.5608 mL	3.9021 mL
	50 mM	0.1249 mL	0.6243 mL	1.2487 mL	3.1217 mL
	60 mM	0.1041 mL	0.5203 mL	1.0406 mL	2.6014 mL
	80 mM	0.0780 mL	0.3902 mL	0.7804 mL	1.9511 mL
	100 mM	0.0624 mL	0.3122 mL	0.6243 mL	1.5608 mL

H-Ala-Ala-OH Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H-Ala-Ala-OH1948-31-8L-Alanyl-L-alanine Ala-AlaAmino Acid DerivativesmTNF-overexpressing K562 cellsglucocorticoid receptor modulatorwild-type K562 cellsmouse anti-TNF antibodymTNF-overexpressing cellsantibody-drug conjugates (ADCs)human intestinal oligopeptide transporter (PEPT1/SLC15A1)wild-type cellsPEPT1-overexpressing epithelial cellsPEPT1Inhibitorinhibitorinhibit

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H-Ala-Ala-OH (Synonyms: L-Alanyl-L-alanine; Ala-Ala)

Other Forms of H-Ala-Ala-OH:

H-Ala-Ala-OH Related Antibodies

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•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

H-Ala-Ala-OH Related Antibodies

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