HRP-Streptavidin 

Sulfonylurea Receptor HRP-Streptavidin is a biotin-binding signal amplifier designed to bind tightly with biotinylated anti-TSH monoclonal antibodies, thereby constituting a key component of the signal amplification system in immunoassays. HRP-Streptavidin is formed by the covalent coupling of Horseradish Peroxidase (HRP) with Streptavidin; it combines the highly efficient catalytic activity of the enzyme with the high affinity of Streptavidin for biotin, making it a potent and sensitive tool for signal amplification. HRP-Streptavidin is suitable for use in Western Blotting, ELISA, and other detection techniques^[1][2].

HRP-Streptavidin conjugate is successfully synthesized via periodate oxidation of HRP and coupling to streptavidin, forming a stable conjugate capable of signal amplification through biotin-streptavidin binding^[1].
HRP-Streptavidin (1:500 dilution; 1 h) enables sensitive and precise detection of the 35-mer HBV DNA fragment, with a limit of detection of 3 pM, a working range of 6-100 pM, a sensitivity of 1.6 × 10⁵ RLU/pM, and CV values below 6%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solid

White to off-white

[HRP-Streptavidin]

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Lin Z, et al. Development of a sensitive, rapid, biotin-streptavidin based chemiluminescent enzyme immunoassay for human thyroid stimulating hormone. Talanta. 2008;75(4):965-972.  [Content Brief]

  [2]. Sakharov I, et al. Microplate Chemiluminescent Assay for DNA Detection Using Apoperoxidase-Oligonucleotide as Capture Conjugate and HRP-Streptavidin Signaling System. Sensors (Basel). 2018;18(4):1289. Published 2018 Apr 23.  [Content Brief]