I2959 

I2959 is widely used as a photoinitiator for photochemical cross-linking of hydrogels, and the combination of I2959 and CMA hydrogel has been used as a bio-ink for biological 3D printing applications. In addition, I2959 has a high free radical generation efficiency and is cytotoxic to rapidly dividing cell lines^[1].

Method for encapsulation of Saos-2 cells in photochemically cross-linked CMA hydrogels^[1]:
1. Human osteosarcoma cell line (Saos-2 cells) are propagated in RPMI medium containing 15% FBS, 1% L-glutamine, and 1% penicillin/streptomycin.
2. Cells are suspended in neutralized CMA solution (containing I2959 at 0.02% or 0.1% concentration).
3. 45 µL of cell suspension is added to each well of a 96-well plate and incubated at 37°C for 30 min for gelation (5000 cells/gel).
4. Gels are exposed to UV light (365 nm) for two different times (1 min or 10 min) for photocross-linking. Hydrogels not exposed to UV light were used as controls for cell studies.
5. Culture the cell-encapsulated hydrogels in α-MEM medium containing 50 µg/mL ascorbic acid, 10 mM β-glycerophosphate, 10% FBS, and 1% penicillin/streptomycin for up to 14 days.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

224.26

C₁₂H₁₆O₄

106797-53-9

Solid

White to off-white

CC(C)(O)C(=O)C1=CC=C(OCCO)C=C1

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Thuy-Uyen Nguyen, et al. Photochemically crosslinked cell-laden methacrylated collagen hydrogels with high cell viability and functionality. J Biomed Mater Res A. 2019 Jul;107(7):1541-1550.  [Content Brief]