Icafolin-methyl 

Icafolin-methyl is a herbicide and plant β-tubulin (Microtubule/Tubulin) inhibitor. Icafolin-methyl binds to the β-tubulin region of the Colchicine (HY-16569) binding site, thereby blocking the polymerization of plant tubulin. As a non-selective herbicide, Icafolin-methyl exhibits post-emergence activity against weeds in both cool-season and warm-season cropping systems, including resistant ryegrass and darnel biotypes. Icafolin-methyl can be used for herbicide research^[1].

Icafolin-methyl (10 nM; 2 weeks) strongly inhibits Arabidopsis thaliana Col-0 seedling growth, inducing morphological abnormalities consistent with mitotic disruption^[1].
Icafolin-methyl (100 nM; 3 days) inhibits tobacco BY-2 cell replication, induces isodiametric cell swelling, and disrupts chromatin structure, indicating mitotic disruption via tubulin polymerization inhibition^[1].
Icafolin-methyl (1.6 μM; 3 days) inhibits Chlamydomonas reinhardtii CC1690 mt⁺ cell growth with an EC₅₀ of 1.3 ± 0.1 μM, preventing cell division and inducing massive cell swelling consistent with tubulin polymerization inhibition^[1].
Icafolin-methyl (10 μM; throughout tubulin polymerization reaction) potently inhibits the in vitro polymerization of purified tobacco BY-2 tubulin, confirming its mode of action as a tubulin polymerization inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

380.34

C₁₈H₁₈F₂N₂O₅

2254752-21-9

COC([C@H](OC1)C[C@H]1NC([C@]2(CC(C3=CC(F)=CC(F)=C3)=NO2)C=C)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Haaf KB, et al. The novel herbicide icafolin-methyl is a plant-specific inhibitor of tubulin polymerization. Pest Manag Sci. 2025;81(5):2652-2661.  [Content Brief]