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  3. IKKγ NBD Inhibitory Peptide TFA

IKKγ NBD Inhibitory Peptide TFA 

Cat. No.: HY-P1847A Purity: 99.33%
COA Handling Instructions

IKKγ NBD Inhibitory Peptide TFA is a highly specific inhibitor NF-κB inhibitor. IKKγ NBD Inhibitory Peptide TFA acts by disrupting the interaction between IKKγ/NEMO-binding domain (NBD) with IKKα and IKKβ, thus blocking TNF-α-induced NF-kB activation. IKKγ NBD Inhibitory Peptide TFA could significantly suppresses inflammation and ameliorate the cerebral ischemia-induced neurological deficits.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

IKKγ NBD Inhibitory Peptide TFA Chemical Structure

IKKγ NBD Inhibitory Peptide TFA Chemical Structure

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5 mg USD 309 In-stock
10 mg USD 480 In-stock
50 mg USD 1400 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

IKKγ NBD Inhibitory Peptide TFA is a highly specific inhibitor NF-κB inhibitor. IKKγ NBD Inhibitory Peptide TFA acts by disrupting the interaction between IKKγ/NEMO-binding domain (NBD) with IKKα and IKKβ, thus blocking TNF-α-induced NF-kB activation. IKKγ NBD Inhibitory Peptide TFA could significantly suppresses inflammation and ameliorate the cerebral ischemia-induced neurological deficits[1][2][3].

In Vitro

In a canonical pathway, NF kappa B activation depends on the IKK complex activity, which is formed by three subunits (IKKκ and IKKβ and IKKγ/NEMO), thus the KKγ NBD Inhibitory Peptide TFA inhibits TNF-α-induced NF-kB activation[2].
IKKγ NBD Inhibitory Peptide TFA (10 μM; 90 min) prevents Doxorubicin (HY-15142A)-induced (15 μM; 4 h) p65 phosphorylation in BT-474 cells[2].
IKKγ NBD Inhibitory Peptide TFA (10 μM; 48 h) slightly inhibits BT-474 cell viability while it markedly enhances the effects on Doxorubicin[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

IKKγ NBD Inhibitory Peptide TFA (40 μg/4 μL; ICV; single dose; 2 h before MCAO) exhibits neuroprotective effect with reduction in DNA fragmentation, and its ischemic brain damage reduction mechanism be attributed to reduction in inflammation following ischemic injury in middle cerebral artery occlusion (MCAO) rats model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male Sprague-Dawley rats (255 g) with middle cerebral artery occlusion (MCAO)[3]
Dosage: 40 μg/4 μL
Administration: Intracerebroventricular injection; 2 h before MCAO induction
Result: Significantly reduced striatal IL-1b level in IKK-NBD peptide treated rats.
Also resulted in reduced staining of microglial OX-42 and protection of BBB from ischemic insult.
Molecular Weight

3807.32

Formula

C172H260N49F3O44S1

Appearance

Solid

Color

White to off-white

Sequence Shortening

DRQIKIWFQNRRMKWKKTALDWSWLQTE

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation

Purity: 99.33%

References
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IKKγ NBD Inhibitory Peptide TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
IKKγ NBD Inhibitory Peptide TFA
Cat. No.:
HY-P1847A
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