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  5. AntiFade Mounting Medium
  6. AntiFade Mounting Medium

AntiFade Mounting Medium 

Cat. No.: HY-K1042
Manual COA SDS Technical Support

MCE AntiFade Mounting Medium can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect.

Tamaño Precio Stock Cantidad
5 mL En stock
25 mL En stock
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AntiFade Mounting Medium Recomendaciones destacadas:

29 Publications Citing Use of MCE AntiFade Mounting Medium

Cell Imaging/Staining
IF

    AntiFade Mounting Medium purchased from MCE. Usage Cited in: J Hazard Mater. 2025 Jan 27:488:137417.  [Abstract]

    Observation of GFP fluorescence within cells of strain KTU-PDG by confocal microscope. Red fluorescence produced by cell membrane-bound FM4–64/L stain (10 μM; 37℃ for 45 min;15 μL samples were sealed with 1 μL AntiFade Mounting). Scan bar: 5 μm.

    AntiFade Mounting Medium purchased from MCE. Usage Cited in: Free Radic Biol Med. 2025 Aug 1:235:231-247.  [Abstract]

    Representative result of immunofluorescence assays (the coverslips were mounted onto slides with antifade mounting medium) to detect GPX4 expression in SUM159 cells after treatment with indicated concentrations of RES for 24 h, and the quantitative data is presented.

    AntiFade Mounting Medium purchased from MCE. Usage Cited in: Emerg Microbes Infect. 2025 Dec;14(1):2542246.  [Abstract]

    Cell specificity in the different tGFP-TBEV infected brain regions. Fluorescent images of mouse brain tissue sections showing various cell types within infected regions. Tissue slices were immunolabelled with anti-calbindin (red)/anti-NeuN (red)/anti-DCX antibodies/anti-GFAP antibodies/anti-Iba1 antibodies. The immunostained tissue slices were mounted with AntiFade mounting medium and stored at 4℃ until imaging.

    AntiFade Mounting Medium purchased from MCE. Usage Cited in: Commun Biol. 2025 Jan 9;8(1):29.  [Abstract]

    IF staining images showing the expression of Pax2a and Pcna in control and lima1a-/- kidneys at 7 dpi. Slides were mounted using an anti-fade mounting medium. White dashed box: Pax2 expressing RPC aggregates, and white arrows: proliferating Pax2-positive cells.

    AntiFade Mounting Medium purchased from MCE. Usage Cited in: Int Immunopharmacol. 2026 Jan 1;168(Pt 1):115785.  [Abstract]

    RAW264.7 cells were transfected with either OE-GCN2 or OE-NC and exposed to 1 μg/mL LPS for 24 h. Immunofluorescent staining of NLRP3 (green) in RAW264.7 cells. Mounted with antifade mounting medium for microscopic imaging.

    • Descripciòn

    • Almacenamiento

    • Protocolo

    • Componentes

    • Documentación

    Description
    & Advantages

    MCE AntiFade Mounting Medium is a sealing reagent used to slow the fluorescence quenching. This product can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect.

    Almacenamiento

    -20°C, 1 year

    Protocolo

    For adherent cell samples:

    1.1 Discard as much liquid as possible after staining.

    1.2 Add one drop of Antifade Mounting Medium to the slide and carefully cover the coverslip containing cells. Be careful to avoid bubbles.

    1.3 Detect the fluorescence by fluorescence microscope.

    For tissue section samples:

    2.1 Discard as much liquid as possible after staining.

    2.2 Add one drop of Antifade Mounting Medium to the tissue and carefully cover the coverslip. Be careful to avoid bubbles.

    2.3 Detect the fluorescence by fluorescence microscope.

    For other samples:

    Other samples are processed with reference to adherent cell samples or tissue section samples.

    Componentes
    Components HY-K1042-5 mL HY-K1042-25 mL
    AntiFade Mounting Medium 5 mL 25 mL
    Documentación

    Manual (163 KB) SDS (311 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Nombre del producto:
    AntiFade Mounting Medium
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    HY-K1042
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