GSH/GSSG Assay Kit 

MCE Glutathione GSH/GSSG Assay Kit is designed to accurately measure total, reduced (GSH) and oxidized (GSSG) glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to the glutathione concentration in the sample. This kit can also be used to measure oxidized glutathione (GSSG) by using a specific protocol which first scavenges all existing GSH using 1-methyl-2-vinylpyridinium triflate as a scavenger reagent. The assay method has a linear detection range of 0.1-3 μM GSH equivalents, with a lower detection limit of 10 nM GSH equivalents.

MCE GSH/GSSG Assay Kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, and tissue and cell extracts.

-20℃, 1 year

After opening, it is stable for up to 2 months at -20℃

Avoid repeated freezing and thawing