2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Metabolism Assay Kit
  6. GSH/GSSG Assay Kit

GSH/GSSG Assay Kit 

Cat. No.: HY-K0311
Manual COA SDS Technical Support

MCE GSH/GSSG Assay Kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, and tissue and cell extracts.

Tamaño Precio Stock Cantidad
100 T En stock
or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

  • Descripciòn

  • Almacenamiento

  • Componentes

  • Documentación

Description
& Advantages

MCE Glutathione GSH/GSSG Assay Kit is designed to accurately measure total, reduced (GSH) and oxidized (GSSG) glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to the glutathione concentration in the sample. This kit can also be used to measure oxidized glutathione (GSSG) by using a specific protocol which first scavenges all existing GSH using 1-methyl-2-vinylpyridinium triflate as a scavenger reagent. The assay method has a linear detection range of 0.1-3 μM GSH equivalents, with a lower detection limit of 10 nM GSH equivalents.

MCE GSH/GSSG Assay Kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, and tissue and cell extracts.
Almacenamiento

-20℃, 1 year

After opening, it is stable for up to 2 months at -20℃

Avoid repeated freezing and thawing
Componentes
Components HY-K0311-100 T
Enzyme Solution 20 U
Coenzyme 4.4 mg
Substrate (DTNB) 4.4 mg
Standard GSH 0.123mg
Standard GSSG 0.123mg
Masking Solution 3.3 mg
Quenching Solution 0.57 mg
Extraction Buffer 15 mL
Buffer Solution 30 mL
Documentación

Manual (6862 KB) SDS (4603 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glutathione Colorimetric Detection KitGSH+GSSG/GSH Assay KitGSH+GSSG/GSH Assay ColorimetricGSH and GSSG Assay KitFerroptosis AssayRedox AssayFerroptosis

Productos vistos recientemente:

Consulta para venta a granel

Your information is safe with us. * Required Fields.

Nombre del producto

  

Requested Quantity *

Nombre del solicitante *

 

Saludo

Direcciòn del E-mail *

Número de teléfono *

Department

 

Nombre de la Organizaciòn *

City

Provincia

Country or Region *

      

Observaciones

Consulta para venta a granel

Inquiry Information

Nombre del producto:
GSH/GSSG Assay Kit
Cat. No.:
HY-K0311
Cantidad:
MCE Japan Authorized Agent:

Request for COA Report

Please fill out this form to request the COA report. We will send it to your email within 3 working days. Your information is safe with us. * Required Fields.

.

Product Name

  

Lot # *

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Remarks

Request for COA Report

We have received your request and will respond to you as soon as possible.