2. MCE-Kits
  3. Cell Biology
  4. Cell Analysis
  5. Metabolism Assay Kit
  6. GSH/GSSG Assay Kit

GSH/GSSG Assay Kit 

Art. -Nr.: HY-K0311
Manual COA SDS Technical Support

MCE GSH/GSSG Assay Kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, and tissue and cell extracts.

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  • Beschreibung

  • Speicherung

  • Komponenten

  • Dokumentation

Description
& Advantages

MCE Glutathione GSH/GSSG Assay Kit is designed to accurately measure total, reduced (GSH) and oxidized (GSSG) glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to the glutathione concentration in the sample. This kit can also be used to measure oxidized glutathione (GSSG) by using a specific protocol which first scavenges all existing GSH using 1-methyl-2-vinylpyridinium triflate as a scavenger reagent. The assay method has a linear detection range of 0.1-3 μM GSH equivalents, with a lower detection limit of 10 nM GSH equivalents.

MCE GSH/GSSG Assay Kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, and tissue and cell extracts.
Speicherung

-20℃, 1 year

After opening, it is stable for up to 2 months at -20℃

Avoid repeated freezing and thawing
Komponenten
Components HY-K0311-100 T
Enzyme Solution 20 U
Coenzyme 4.4 mg
Substrate (DTNB) 4.4 mg
Standard GSH 0.123mg
Standard GSSG 0.123mg
Masking Solution 3.3 mg
Quenching Solution 0.57 mg
Extraction Buffer 15 mL
Buffer Solution 30 mL
Dokumentation

Manual (6862 KB) SDS (4603 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glutathione Colorimetric Detection KitGSH+GSSG/GSH Assay KitGSH+GSSG/GSH Assay ColorimetricGSH and GSSG Assay KitFerroptosis AssayRedox AssayFerroptosis

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GSH/GSSG Assay Kit
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