Human Esophageal Organoid Kit 

MCE Human Esophageal Organoid Kit includes a basic culture medium for human esophageal organoids, as well as culture supplements, and is designed for the establishment and long-term maintenance of human esophageal organoids.

The self-renewal of the esophageal epithelium is driven by the proliferation of stem cells and squamous cells. Human esophageal organoids recapitulate key features of the native esophagus, including its architecture, cellular composition, and self-renewal dynamics, making them a powerful tool for advanced studies of human esophageal homeostasis and disease.

Basal Medium A: 4°C, 1 year. Shipping with blue ice.

Supplement B (50×) & Supplement C (250×): -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry a ice.

1. Cells need to be kept sterile when extracted from primary tissue to avoid contamination from subsequent experiments.

2. Observe the fragmentation status of the organoid during passaged dissociation, and terminate the dissociation when small cell clusters (10-50 cells) appear to avoid prolonging the subsequent growth viability of the organoid.

3. Operations involving MCE Basement Membrane Matrix need to be kept at low temperature throughout. MCE Basement Membrane Matrix should be injected rapidly into the cell culture wells after resuspension with the cells, while avoiding air bubbles.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.