Human Liver Ductal (Differentiation) Organoid Kit 

MCE Human Liver Ductal (Differentiation) Organoid Kit includes a basic culture medium for Human Liver Ductal (Differentiation) organoids, as well as culture supplements, and is designed for the establishment and long-term maintenance of human liver ductal organoids (hLDs) derived from adult stem cells.

The self-renewal of the ductal epithelium is sustained by the proliferation of stem cells and their progenitors residing in the liver. The human liver ductal organoids (hLDs) recapitulate key features of the native ductal epithelium, including tissue architecture, cellular composition, and self-renewal dynamics. As such, they represent a powerful in vitro model for advancing studies on human liver development and disease. Under appropriate differentiation conditions, hLDs can be induced to differentiate into hepatocyte-like cells.

Human liver ductal organoids (hLDs) cultured in Human Liver Ductal Organoid Expansion Medium predominantly consist of cholangiocytes. Upon switching to the Differentiation Medium, these organoids are capable of differentiating into hepatocyte-like cells, which express hepatocyte-specific markers such as Albumin, Ttr, and Cyp3A4.

Basal Medium A: 4°C, 1 year. Shipping with blue ice.

Supplement B (50×) & Supplement C (250×) & Supplement D (250×): -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry a ice.

1. Observe the fragmentation status of the organoid during passaged dissociation, and terminate the dissociation when small cell clusters (10-50 cells) appear to avoid prolonging the subsequent growth viability of the organoid.

2. Operations involving MCE Basement Membrane Matrix need to be kept at low temperature throughout. MCE Basement Membrane Matrix should be injected rapidly into the cell culture wells after resuspension with the cells, while avoiding air bubbles.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.