ROS Assay Kit 

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s and Parkinson′s disease, cancer, and aging.

MCE ROS Assay Kit utilizes the fluorescent probe DCFH-DA to detect ROS. The principle is that DCFH-DA initially has no fluorescence and can penetrate the cell membrane, be hydrolyzed by intracellular esterases into DCFH which cannot penetrate the cell membrane, and the intracellular ROS can oxidize the non-fluorescent DCFH to generate fluorescent DCF. This fluorescence is then detected using a fluorescence microscope, flow cytometer, or laser confocal microscope to analyze the levels of ROS within the cell.

Features of MCE ROS (Reactive Oxygen Species) Assay Kit

1. Positive control: Provides an ROS positive control reagent (Rosup, 50 mg/mL)

2. High sensitivity: High sensitivity, low background and wide linear range

-20℃, 1 year

Avoid repeated freeze-thaw cycles