Janelia Fluor® 669, SE  (Synonyms: JF669, SE; JF669, NHS)

Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm)^[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

JF669 undergoes rapid conversion to thioether product (<5 min to completion) in organic solvents, where the dye predominantly adopts the closed form^[1].
JF669 displays comparatively excellent photostability, retaining 97% fluorescence after the same number of cycles^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

693.76

C₃₄H₃₀F₃N₃O₆SSi

2127150-20-1

Solid

Off-white to light yellow

669

640

C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=C(C(SCC(ON6C(CCC6=O)=O)=O)=C(C(F)=C5C([O-])=O)F)F)=C1C=CC2=[N+]7CCC/7

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Jonathan B Grimm, et al. General Synthetic Method for Si-Fluoresceins and Si-Rhodamines. ACS Cent Sci. 2017 Sep 27;3(9):975-985.  [Content Brief]