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  4. L-012

L-012 is a luminal-based chemiluminescent probe. L-012 sodium salt can detect NADPH oxidase (Nox)-derived superoxide and nitrogen species (reactive oxygen species (ROS) and reactive nitrogen species (RNS)).

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CAS 番号 : 143323-55-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 165 在庫あり
Solution
10 mM * 1 mL in DMSO USD 165 在庫あり
Solid
5 mg $150 在庫あり
10 mg $251 在庫あり
25 mg $430 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 2 publication(s) in Google Scholar

Other Forms of L-012:

L-012 関連抗体

Top Publications Citing Use of Products
Histological Imaging/Staining
Bio/Physico-chemical Assay

    L-012 purchased from MedChemExpress. Usage Cited in: Cell Calcium. 2026 Mar:134:103122.  [Abstract]

    Activity of NOX2 in cells treated with 100ng/ml PMA, PMA+ 30 μM GSK2795039and 10 μM S-023–0515 was measured by L-012 (10 μM, 10 min at 37 °C) luminescence kinetics over 90 min.

    L-012 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2024 Jul 22:28:101163.  [Abstract]

    Live imaging of small animals and quantitative analysis of ROS stained by L012 in periodontal tissue with different treatments (7d).

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    製品説明

    L-012 is a luminal-based chemiluminescent probe. L-012 sodium salt can detect NADPH oxidase (Nox)-derived superoxide and nitrogen species (reactive oxygen species (ROS) and reactive nitrogen species (RNS))[1][2].

    体外実験

    1. Solution preparation[1]
    1.1 Preparation of stock solution
    Solvent: DMSO or ultrapure water
    Concentration: 10 mM is recommended.
    Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2 Assay for ROS Generated by Cell-Free Systems.
    2.1 In the presence of 1 mM hypoxanthine in KRP (Krebs-Ringer phosphate buffer), superoxide is generated by xanthine oxidase (6.4 mU/mlL) at 37°C.
    2.2 Concentrations of L-012, luminol and MCLA used for the assay are 4 μM, 100 μM, and 4 μM, respectively.
    2.3 The hydroxyl radical is generated in KRP by incubating 100 μM DTPA, 1 mM H2O2, and 50 μM FeSO4 at 37°C.
    2.4 Detection using in Luminescence Reader.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-012 関連抗体
    体内実験

    NF-κB-luciferase reporter mice[2].
    Luciferase activity was monitored in vivo by injecting the substrate luciferin.
    L-012 (25 mg/kg in 100 μL) is administered intravenously (iv), intraperitoneally (ip), or subcutaneously (sc).
    Detection using in vivo imaging.
    Image exposure times are between 5 s and 2 min, depending on the signal strength.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    288.69

    分子式

    C13H9ClN4O2
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=C2C(Cl)=NC(C3=CC=CC=C3)=C1N)NNC2=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (346.39 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4639 mL 17.3196 mL 34.6392 mL
    5 mM 0.6928 mL 3.4639 mL 6.9278 mL
    10 mM 0.3464 mL 1.7320 mL 3.4639 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

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    濃度 （終了）

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    体積 （終了）

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.4639 mL 17.3196 mL 34.6392 mL 86.5981 mL
    5 mM 0.6928 mL 3.4639 mL 6.9278 mL 17.3196 mL
    10 mM 0.3464 mL 1.7320 mL 3.4639 mL 8.6598 mL
    15 mM 0.2309 mL 1.1546 mL 2.3093 mL 5.7732 mL
    20 mM 0.1732 mL 0.8660 mL 1.7320 mL 4.3299 mL
    25 mM 0.1386 mL 0.6928 mL 1.3856 mL 3.4639 mL
    30 mM 0.1155 mL 0.5773 mL 1.1546 mL 2.8866 mL
    40 mM 0.0866 mL 0.4330 mL 0.8660 mL 2.1650 mL
    50 mM 0.0693 mL 0.3464 mL 0.6928 mL 1.7320 mL
    60 mM 0.0577 mL 0.2887 mL 0.5773 mL 1.4433 mL
    80 mM 0.0433 mL 0.2165 mL 0.4330 mL 1.0825 mL
    100 mM 0.0346 mL 0.1732 mL 0.3464 mL 0.8660 mL
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    L-012 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-012143323-55-1L012L 012Fluorescent DyeProbesNeutrophilsFree RadicalsDisease PathogenesisChemiluminescenceInhibitorinhibitorinhibit

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    製品名:
    L-012
    製品番号:
    HY-118315
    数量:
    MCE 日本正規代理店:

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