L 012 (sodium salt)
Based on 2 publication(s) in Google Scholar
L 012 sodium salt is a luminal-based chemiluminescent probe. L 012 sodium salt can detect NADPH oxidase (Nox)-derived superoxide and nitrogen species (reactive oxygen species (ROS) and reactive nitrogen species (RNS)).
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- 純度: 99.90%
- CAS 番号: 143556-24-5
- 分子式: C13H8ClN4NaO2
- 分子量:310.67
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保管条件:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 L 012 sodium salt
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Bio/Physico-chemical Assay
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Histological Imaging/Staining
生物活性
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[1]
1.1 Preparation of stock solution
Solvent: DMSO or ultrapure water
Concentration: 10 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Assay for ROS Generated by Cell-Free Systems
2.1 In the presence of 1 mM hypoxanthine in KRP (Krebs-Ringer phosphate buffer), superoxide is generated by xanthine oxidase (6.4 mU/mlL) at 37°C.
2.2 Concentrations of L 012 sodium salt, luminol and MCLA used for the assay are 4 μM, 100 μM, and 4 μM, respectively.
2.3 The hydroxyl radical is generated in KRP by incubating 100 μM DTPA, 1 mM H2O2, and 50 μM FeSO4 at 37°C.
2.4 Detection using in Luminescence Reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Luciferase activity was monitored in vivo by injecting the substrate luciferin.
L-012 (25 mg/kg in 100 μL) is administered intravenously (iv), intraperitoneally (ip), or subcutaneously (sc).
Detection using in vivo imaging.
Image exposure times are between 5 s and 2 min, depending on the signal strength.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 143556-24-5
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性状 Solid
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分子量 310.67
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分子式 C13H8ClN4NaO2
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Color White to yellow
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SMILES
O=C1C2=C(C(Cl)=NC(C3=CC=CC=C3)=C2N)C(O[Na])=NN1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (2)
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Journal Impact Factor
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Most Recent
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Mater Today Bio
Multifunctional human serum albumin-crosslinked and self-assembling nanoparticles for therapy of periodontitis by anti-oxidation, anti-inflammation and osteogenesis. [Abstract]2024 Jul 22:28:101163. PMID: 39183771
L 012 sodium salt purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2024 Jul 22:28:101163. [Abstract]
Live imaging of small animals and quantitative analysis of ROS stained by L012 in periodontal tissue with different treatments (7d).
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Cell Calcium
A NOX2-independent mechanism of Hv1 channel activation promotes inflammatory cytokine release from BV-2 microglia via intracellular Ca2+ mobilisation. [Abstract]2026 Mar:134:103122. PMID: 41581267
L 012 sodium salt purchased from MedChemExpress. Usage Cited in: Cell Calcium. 2026 Mar:134:103122. [Abstract]
Activity of NOX2 in cells treated with 100ng/ml PMA, PMA+ 30 μM GSK2795039and 10 μM S-023–0515 was measured by L-012 (10 μM, 10 min at 37 °C) luminescence kinetics over 90 min.
溶剤 & 溶解度
DMSO : 50 mg/mL (160.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 3.33 mg/mL (10.72 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (279 KB)
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SDS (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Zielonka J, et al. On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation. Free Radic Biol Med. 2013;65:1310-1314. [Content Brief]
[2]. Kielland A, et al. In vivo imaging of reactive oxygen and nitrogen species in inflammation using the luminescent probe L-012. Free Radic Biol Med. 2009;47(6):760-766. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.2188 mL | 16.0942 mL | 32.1885 mL | 80.4712 mL |
| 5 mM | 0.6438 mL | 3.2188 mL | 6.4377 mL | 16.0942 mL | |
| 10 mM | 0.3219 mL | 1.6094 mL | 3.2188 mL | 8.0471 mL | |
| DMSO | 15 mM | 0.2146 mL | 1.0729 mL | 2.1459 mL | 5.3647 mL |
| 20 mM | 0.1609 mL | 0.8047 mL | 1.6094 mL | 4.0236 mL | |
| 25 mM | 0.1288 mL | 0.6438 mL | 1.2875 mL | 3.2188 mL | |
| 30 mM | 0.1073 mL | 0.5365 mL | 1.0729 mL | 2.6824 mL | |
| 40 mM | 0.0805 mL | 0.4024 mL | 0.8047 mL | 2.0118 mL | |
| 50 mM | 0.0644 mL | 0.3219 mL | 0.6438 mL | 1.6094 mL | |
| 60 mM | 0.0536 mL | 0.2682 mL | 0.5365 mL | 1.3412 mL | |
| 80 mM | 0.0402 mL | 0.2012 mL | 0.4024 mL | 1.0059 mL | |
| 100 mM | 0.0322 mL | 0.1609 mL | 0.3219 mL | 0.8047 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L 012 sodium salt 関連分類
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